Establishment of quantitative RT-PCR method to defect drug-resistant-related genes

耐药相关基因缺陷定量RT-PCR方法的建立

• 批准号: 09672348
• 负责人: FUNATO Tadao
• 金额: $ 2.37万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672348/
• 关键词: drug resistance; leukemia; real-time RT-PCR; Multidrug reistance; cytosine arabinoside; 多剤耐性遺伝子(MDR); 薬剤耐性遺伝子; 遺伝子診断

The failure of chemotherapy to eradicate human leukemic cells is often due to the development of drug resistance. MDR (multidrug resistance) whose one form of drug resistance results from a decreased intracellular accumulation of the drugs, most often mediated by the overexpression of P-glycoprotein. Multidrug-related protein (MRP) also related to pump function of cell membrane in acute leukemia. Decreased deoxycytidine kinase (dCK) gene was observed in cytosine arabinoside resistant leukemia cells (ara-C) which we have established. We have developed the new quantitative assay based on real-time reverse transcriptase polymerase chain reaction (RT-PCR) to mesure expression of drug-resistance related genes such as MDR-1, MRP, and dCK in clinical samples. These results indicates that real-time RT-PCR system is a releable method to quantitatively determine drug resistant genes expression, it may be to predict responsiveness to chemotherapy by using this technique.

化疗不能根除人类白血病细胞通常是由于耐药性的发展。MDR（多药耐药），其中一种耐药形式是由于细胞内药物积累减少，最常由P-糖蛋白的过度表达介导。多药相关蛋白（MRP）也与急性白血病细胞膜泵功能有关。在我们建立的阿糖胞苷耐药白血病细胞（ara-C）中观察到脱氧胞苷激酶（dCK）基因的减少。我们建立了一种新的基于实时荧光定量RT-PCR的检测临床标本中MDR-1、MRP、dCK等耐药相关基因表达的方法。结果表明，实时荧光定量RT-PCR技术是一种可行的定量检测耐药基因表达的方法，可用于预测化疗的敏感性。

项目成果

舩渡 忠男,他: "がんの薬剤耐性の遺伝子診断"臨床病理. 48. 162-166 (2000)

Tadao Funato 等：“癌症耐药性的基因诊断”《临床病理学》48. 162-166 (2000)。

In vitro leukemia cell models of Ara-C resistance

• DOI: 10.1016/s0145-2126(00)00016-3
• 发表时间: 2000-06-01
• 期刊: LEUKEMIA RESEARCH
• 影响因子: 2.7
• 作者: Funato, T;Satou, J;Sasaki, T
• 通讯作者: Sasaki, T

籐巻 慎一,他: "定量RT-PCRにおけるRNA基準量の設定に関する検査"臨床病理. 48. 54-59 (2000)

Shinichi Satomaki 等人：“定量 RT-PCR 中设置 RNA 参考量的测试”《临床病理学》48. 54-59 (2000)。

籐巻 慎一,他: "簡便な定量的PCRシステムによる白血病MRDの遺伝子診断"International J Haemtol. 69suppl. 67 (1999)

Shinichi Satomaki 等人：“使用简单定量 PCR 系统进行白血病 MRD 的基因诊断”International J Haemtol 69 (1999)。

A quantitative reverse transcriptase polymerase chast reaction method for the detection of leukemic cells with t(8:21) peripheral blood.

一种定量逆转录聚合酶链反应检测t(8:21)外周血白血病细胞的方法。

• 发表时间: 2000
• 期刊: Europian Journal of Haematology (in press)
• 作者: Fujimaki S;Funato;T;et al.
• 通讯作者: et al.