2-Cys Prx and PrxIIE as redox sensors, signal integrators and transmitters

过氧化物还原素（Prx）是一种广泛存在于不同细胞区室的氧化还原蛋白，具有抗氧化剂作用，并参与复杂的信号传导功能。该项目将阐明叶绿体过氧化物氧化酶2-Cys Prx和PrxllE作为检测和传递氧化还原信息到不同目标功能的调节枢纽的功能。该项目将结合(1)详细的体外生化和(2)原位蛋白质分析，(3)转基因方法进行验证，(4)两个信号中心的功能分化，(5)将为调节状态预测和建模奠定基础。一方面，定量研究抑制2-Cys Prx/核酸和激活2-Cys Prx/果糖-1,6-磷酸酶相互作用等新的调控特征，比较植物、蓝藻和人类2-Cys Prx （Prdxl、PrdxS）对2-Cys Prx/DNA的干扰。综合分析相互作用和活性将与体内翻译后状态联系起来，以预测2-Cys Prx在不同环境条件下的调节作用。同样，PrxllE激活状态的调节将在氧化还原和活性氮（NO，过氧化物矿）的依赖下进行分析，以评估Prx在细胞驯化中活性氧和活性氮依赖信号之间的串音中的作用。

Peroxiredoxins (Prx) are ubiquitously occurring redox proteins abundantly present in different cell compartments that serve as antioxidants and are implicated in a complex signaling function. The project will elucidate the function of the chloroplast peroxiredoxins 2-Cys Prx and PrxllE as regulatory hubs detecting and transmitting redox information to diverse target functions. The project will combine (1) detailed in vitro biochemical and (2) in situ protein analyses, (3) transgenic approaches for validation, (4) a functional differentiation of both signaling hubs and (5) will lay ground for regulatory state prediction and lateron for modelling. On the one hand novel regulatory features such as the inhibitory 2-Cys Prx/nucleic acid and the activating 2-Cys Prx/fructose-1,6-phosphatase interactions will be studied on a quantitative basis, the former 2-Cys Prx/DNA interference will be compared among plant, cyanobacteria and human 2-Cys Prx (Prdxl, PrdxS). The comprehensive analysis of interactions and activity will be linked to the posttranslational state in vivo to predict the regulatory role of the 2-Cys Prx under divergent environmental conditions. Likewise, regulation of the PrxllE activation state will be analyzed in dependence of redox and reactive nitrogen species (NO, peroxinitrite) in order to assess the proposed function of Prx in the crosstalk between reactive oxygen and reactive nitrogen dependent signaling in cell acclimation.