2-Cys Prx and PrxIIE as redox sensors, signal integrators and transmitters

过氧化物酶（Peroxiredoxins，Prx）是一种广泛存在于不同细胞中的氧化还原蛋白，具有抗氧化作用，参与复杂的信号传导功能。该项目将阐明叶绿体过氧化物酶2-Cys Prx和PrxllE作为检测和传递氧化还原信息到不同目标功能的调节中心的功能。该项目将结合联合收割机（1）详细的体外生物化学和（2）原位蛋白质分析，（3）转基因方法的验证，（4）功能分化的两个信号枢纽和（5）将奠定基础的监管状态预测和稍后的建模。一方面，对2-Cys Prx/核酸的抑制性和2-Cys Prx/果糖-1，6-磷酸酶的激活性等新的调控特征进行定量研究，并对植物、蓝藻和人2-Cys Prx（Prdxl，PrdxS）的2-Cys Prx/DNA干扰进行比较。相互作用和活性的综合分析将被链接到体内的翻译后状态，以预测在不同的环境条件下的2-Cys Prx的调节作用。同样，将根据氧化还原和活性氮物质（NO，过氧亚硝酸盐）分析PrxIIE活化状态的调节，以评估Prx在细胞适应中活性氧和活性氮依赖性信号传导之间的串扰中的拟议功能。

Peroxiredoxins (Prx) are ubiquitously occurring redox proteins abundantly present in different cell compartments that serve as antioxidants and are implicated in a complex signaling function. The project will elucidate the function of the chloroplast peroxiredoxins 2-Cys Prx and PrxllE as regulatory hubs detecting and transmitting redox information to diverse target functions. The project will combine (1) detailed in vitro biochemical and (2) in situ protein analyses, (3) transgenic approaches for validation, (4) a functional differentiation of both signaling hubs and (5) will lay ground for regulatory state prediction and lateron for modelling. On the one hand novel regulatory features such as the inhibitory 2-Cys Prx/nucleic acid and the activating 2-Cys Prx/fructose-1,6-phosphatase interactions will be studied on a quantitative basis, the former 2-Cys Prx/DNA interference will be compared among plant, cyanobacteria and human 2-Cys Prx (Prdxl, PrdxS). The comprehensive analysis of interactions and activity will be linked to the posttranslational state in vivo to predict the regulatory role of the 2-Cys Prx under divergent environmental conditions. Likewise, regulation of the PrxllE activation state will be analyzed in dependence of redox and reactive nitrogen species (NO, peroxinitrite) in order to assess the proposed function of Prx in the crosstalk between reactive oxygen and reactive nitrogen dependent signaling in cell acclimation.