Theiler's virus as a vector for foreign gene delivery into the CNS

我们报告了DA strain of Theiler's murine encephalomyelitis病毒（TMEV）在宏观中生长的地方，如果感染，因此维护基因组。在另一方面，TMEV的GDVIII菌株不能生长，不能维持基因组（J Virol 71 ： 729-733， 1997年）。这种神经特异性、宿主细胞依赖性病毒的增长仅在宏观细胞中观察到，而不是在其他细胞类型中观察到（微生物免疫， 43 ： 885-892， 1999）。重组和突变病毒研究也演示了L-D1 * L-D1蛋白，它是在框外，并从L编码区域内的替代AUG中翻译出来，为这一现象发挥了一个重要的作用（J Virol 72 ： 4950-4955， 1998）。我们终于插入了淋巴毒素（LT）基因进入了L编码区域的DA strain of TMEV和生成重组病毒。Western Blotting and Enzyme-linked Immunosorbent assay演示BHK-21细胞感染了重组病毒的LT表达。在添加中，显示出对L-929细胞的细胞毒性。因此，目前的研究提供了TMEV的起源，作为一个载体，用于将外国基因输送到中枢神经系统。

We reported that DA strain of Theiler's murine encephalomyelitis virus (TMEV) grows in macrophages where persists in the chronic stage if infection, therefore maintaining the genome. On the other hand, GDVII strain of TMEV does not grow there, and cannot maintain the genome (J Virol 71 : 729-733, 1997). Such a strain-specific, host cell-dependent virus growth was observed only in macrophages, but not in other types of cells (Microbiol Immunol, 43 : 885-892, 1999). Recombinant and mutant virus study also demonstrated that LィイD1*ィエD1 protein, which is out-of-frame with the polyprotein and translated from the alternative AUG within the L coding region, plays an important role for this phenomenon (J Virol 72 : 4950-4955, 1998). We finally inserted lymphotoxin (LT) gene into the L coding region of DA strain of TMEV and generated the recombinant virus. Western blotting and enzyme-linked immunosorbent assay demonstrated the expression of LT in BHK-21 cells infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells. Therefore, the present study provides a beginning of the use of TMEV as a vector for foreign gene delivery into the CNS.