Analysis of molecular response in the heart to emotional stress

心脏对情绪压力的分子反应分析

• 批准号: 09670740
• 负责人: UEYAMA Takashi
• 金额: $ 1.6万
• 依托单位: Wakayama Medical Collage
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670740/
• 关键词: Emotional stress; Heart; Electrocardiogram; Immediate early genes; Natriuretic peptide; Heat shock protein; Adrenoceptor; Signal transduction; Emotional stress; Heart; Electrocardiogram; Immediate early genes; ANP; HSP70; Adrenoceptor; cros

Emotional stress, and associated activation of adrenergic system underlie cardiac accidents. Immobilization stress of rats, which produces activation of the sympatho-adrenal medullary system, induced ischemic electrocardiographic changes. Corresponding to these changes, immediate early genes/transcriptional factors (IEGs) were expressed in the smooth muscle cells of coronary arteries, enodothelial cells and myocardium. Furthermore, mRNA for heat shock protein 70 was induced in the aortic and coronary smooth muscles cells from 30min to 3h after onset of stress. Natriuretic peptide genes (ANP and BNP) were also upregulated. Sequential expression of these genes can be considered as an adaptive response to stress. Not a single but synergistic blocking of alpha and beta adrenoceptors blocked the IEGs expressions elicited by stress. Either alpha or beta agonist upregulated IEGs in the perfused heart. Activation of alpha or beta adrenoceptors is the primary trigger of stress-induced molecular and electrophysiological changes in the heart. Crosstalk of signal transduction between downstream of alpha and beta adrenoceptors in these cardiac cells is also suggested.

情绪压力和相关的肾上腺素能系统激活是心脏事故的基础。大鼠制动应激引起交感-肾上腺髓质系统激活，引起缺血性心电图改变。与这些变化相对应，即刻早期基因/转录因子（IEGs）在冠状动脉平滑肌细胞、内皮细胞和心肌中表达。此外，热休克蛋白70的mRNA诱导的主动脉和冠状动脉平滑肌细胞从30分钟至3小时后开始的应激。利钠肽基因（ANP和BNP）也上调。这些基因的顺序表达可以被认为是对压力的适应性反应。不是一个单一的，但协同阻断α和β肾上腺素受体阻断IEGs的表达引起的压力。α或β激动剂上调灌注心脏中的IEGs。α或β肾上腺素受体的激活是心脏中应激诱导的分子和电生理变化的主要触发因素。在这些心脏细胞中的α和β肾上腺素受体的下游之间的信号转导的串扰也被建议。

项目成果

Ueyama. T. et al.: "Water immersion-restraint stress induces exression of immediate-early genes in gastrointestinal tract of rats"American Journal of Physiology. 275. G287-G295 (1998)

上山。

Shuji Hatakeyama: "NO Synthease containing magnocellular neurons of the rat hypothalamus synthesize oxtocin and vasopressin and express Fos following stress stimuli"J. Chem. Neuroanat.. 11. 243-246 (1996)

Shuji Hatakeyama：“大鼠下丘脑大细胞神经元含有一氧化氮合成酶，在应激刺激后合成催产素和加压素并表达 Fos”。

Santa Umemoto: "Glucocorticoid administration as well as repeated stress affects the subsequent acute immobilization stress-induced expression of immediate early genes but not that of NGFI-A"Neuroscience. 80. 763-773 (1997)

Santa Umemoto：“糖皮质激素的施用以及重复的应激会影响随后的急性固定应激诱导的立即早期基因的表达，但不会影响 NGFI-A 的表达”神经科学。

Takashi Ueyama: "Water immersion/restraint stress induces expression of immediate early genes in the gastrointestinal tract of rats"Am. J. Physiol.. 275(2 Pt 1). G287-G295 (1998)

Takashi Ueyama：“水浸/束缚应激诱导大鼠胃肠道中早期基因的表达”Am。

Takashi Ueyama: "Immobilization stress induced c-fos and c-jun immediate early genes expression in the heart" Life Science. 59 (4). 339-347 (1996)

Takashi Ueyama：“固定应激诱导 c-fos 和 c-jun 在心脏中立即早期基因表达”生命科学。