Molecular phylogenetic analysis on evolution and population diversity of the Iriomote cat and the Tsushima cat

西表猫和对马猫进化和种群多样性的分子系统发育分析

• 批准号: 09640820
• 负责人: MASUDA Ryuichi
• 金额: $ 1.98万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640820/
• 关键词: Iriomote cat; Tsushima cat; Molecular phylogeny; Molecular evolution; Population genetics; Geographic isolation; Genetic diversity

The objective of this project was to understand the evolutionary history and genetic diversity of endangered populations of the Iriomote cat and the Tsushima cat, based on molecular genetic analysis. The results obtained in this study were as follows :1) Sequence analysis of the mitochondrial DNA control region showed no variation in the Iriomote cat population, although this gene is thought to evolve most rapidly among mitochondrial genes.2) Microsatellite DNA analysis showed a remarkably low genetic diversity in both populations of the Iriomote cat and the Tsushima cat, indicating genetic drift and/or inbreeding due to geographic isolation on the Iriomote Island and the Tsushima Island for approximately 0.2 and 0.1 million years, respectively.3) Major histocompatibility complex (MHC) class II DRB locus was examined for the two wildcats and some alleles were obtained. At present, their sequences are being analyzed phylogenetically.4) Some parts of the results of DNA analysis have been exhibited to public at the Wildlife Conservation Center of Tsushima.

本项目的目的是在分子遗传分析的基础上，了解Iriomote猫和Tsushima猫濒危种群的进化历史和遗传多样性。本研究获得的结果如下：1)线粒体DNA控制区的序列分析表明，虽然该基因在线粒体基因组中进化最快，但在Iriomote猫群体中没有变异。2)微卫星DNA分析显示，Iriomote猫和对马猫两个群体的遗传多样性都非常低，表明遗传漂移和/或近亲交配分别是由于Iriomote岛和对马岛上大约20万年和10万年的地理隔离所致。3)检测了这两个野生猫的主要组织相容性复合体(MHC)II类DRB基因座，并获得了一些等位基因。目前，它们的序列正在进行系统发育分析。4)部分DNA分析结果已在对马野生动物保护中心向公众展出。

项目成果

J.Nagata: "Genetic variation and population structure of the Japanrse sika deer(Cervus nippon)in Hokkaido Island,based on mitochondrial D-loop sequences." Molecular Ecology. (in press).

J.Nagata：“基于线粒体 D 环序列的北海道岛日本梅花鹿 (Cervus nippon) 的遗传变异和种群结构。”

S.Ohdachi: "Phylogeny of Eurasian soricine shrew(Insectivora,Mammalia)inferred from the mitochondrial cytochrome b gene sequences." Zoological Science. 14. 527-532 (1997)

S.Ohdachi：“从线粒体细胞色素 b 基因序列推断出欧亚母草鼩鼱（食虫目、哺乳动物）的系统发育。”

増田隆一: "日本産食肉目の種名の検討" 哺乳類科学. 37. 87-93 (1997)

Ryuichi Masuda：“日本食肉动物的物种名称检查”《哺乳动物科学》37. 87-93 (1997)。

増田隆一: "イエネコ・ヤマネコの過去・未来"Biohistory生命誌. 21. 12-13 (1998)

Ryuichi Masuda：“家猫和野猫的过去和未来”生物历史生物历史。 21. 12-13 (1998)

Masuda,R.: "Phylogenetic status of brown bears Ursus arctos of Asia : A preliminary result inferred from mitochondrial DNA control region sequences"Hereditas. 128. 277-280 (1998)

Masuda,R.：“亚洲棕熊 Ursus arctos 的系统发育状况：从线粒体 DNA 控制区序列推断的初步结果”遗传。