Studies on phylogeographic changes and mechanisms of diversification of the Hokkaido sika deer populations using ancient DNA analysis

利用古代DNA分析研究北海道梅花鹿种群的系统发育地理学变化和多样化机制

• 批准号: 15370034
• 负责人: MASUDA Ryuichi
• 金额: $ 9.22万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15370034/
• 关键词: Ezo sika deer; bottleneck; phylogeograph; molecular phylogeny; mitochondrial DNA; diversity; ancient DNA; archaeology

The purposes of the present study are to analyze ancient DNA of sika deer bone remains excavated from archaeological sites in Hokkaido, and to reveal changes of their population structures, phylogeographical history, and mechanisms of diversification, compared with genetic data of the modern populatiuons of sika deer. We identified many haplotypes of mitochondrial DNA (mtDNA) from deer remains of various ages (from the Jomon, Epi-Jomon, and Ainu Culture Periods) and of various areas within Hokkaido. The haplotypes included new ones which had not been found from modern sika deer. From more aged archaeological sites, more new haplotypes were found. Molecular phylogenetic studies including mtDNA of modern Honshu sika deer demonstrated that haplotypes of the Hokkaido sika deer form a common cluster between ancient and modern ones. The results show that genetic diversity in the Hokkaido sika deer population has been reduced since the Jomon Period, and that they have experienced large movement of populations as well as dynamic changes of population sizes such as bottlenecks through environmental changes in past Hokkaido. In addition, we investigated mtDNA of present sika deer and compared the data with those of samples collected ten years ago. As a result, we found the movement of the sika deer populations from eastern to western parts of Hokkaido during the last ten years.

本研究的目的是分析从北海道考古遗址中出土的西卡骨骼遗骸的古代DNA，并与现代西卡种群的遗传数据进行比较，揭示其种群结构的变化、地理历史和多样化机制。我们从北海道不同地区不同年代（绳纹文化、上绳纹文化和阿伊努文化）的鹿遗骸中鉴定出许多线粒体DNA（mtDNA）单倍型。这些单倍型包括了在现代西卡中未发现的新单倍型。包括现代本州岛西卡mtDNA在内的分子系统发育研究表明，北海道西卡的单倍型在古代和现代之间形成了一个共同的聚类。结果表明，北海道西卡种群的遗传多样性自绳纹时代以来一直在减少，并且在过去的北海道经历了大规模的种群迁移以及种群规模的动态变化，如环境变化造成的瓶颈。此外，我们还对现代西卡的线粒体DNA进行了研究，并与10年前采集的样品进行了比较。结果发现，在过去的十年里，西卡种群从北海道的东部向西部迁移。

项目成果

Phylogeography of the Russian flying squirrel (Pteromys volans): implication of refugia theory in arboreal small mammal of Eurasia

• DOI: 10.1111/j.1365-294x.2005.02475.x
• 发表时间: 2005-03
• 期刊: Molecular Ecology
• 影响因子: 4.9
• 作者: T. Oshida;A. Abramov;H. Yanagawa;R. Masuda

Phylogenetic position of the small Kashmir flying squirrel, Hylopetes fimbriatus (≡ Eoglaucomys fimbriatus), in the subfamily Pteromyinae

• DOI: 10.1139/z04-108
• 发表时间: 2004-08
• 期刊: Canadian Journal of Zoology
• 影响因子: 1.4
• 作者: T. Oshida;Chaudhry M. Shafique;S. Barkati;M. Yasuda;N. Hussein;H. Endo;H. Yanagawa;R. Masuda

クマのDNAが語るオホーツク人の交流.

熊 DNA 揭示鄂霍次克相互作用。

• 发表时间: 2005
• 期刊: 日本文化財科学会会報 49
• 作者: Takahashi A.;他8名;増田隆一
• 通讯作者: 増田隆一

Genetic diversity in domestic cats Felis catus of the Tsushima Islands, based on mitochondrial DNA cytochrome b and control region nucleotide sequences

基于线粒体 DNA 细胞色素 b 和控制区核苷酸序列的对马群岛家猫 Felis catus 的遗传多样性

• 发表时间: 2005
• 期刊: Zoological Science 22
• 作者: Daichi Nabata;Daichi Nabara;Ryuichi Masuda;増田隆一;Ryuichi Masuda;Ryuichi Masuda;増田隆一;Tsutomu Tamada
• 通讯作者: Tsutomu Tamada

Ryuichi Masuda: "Molecular phylogeography of the Japanese weasel Mustela itatsi (Mustelidae, Carnivora) and the Siberian weasel M.sibirica on Japanese islands, based on mitochondrial DNA control region variations"Biological Journal of the Linnean Society.

Ryuichi Masuda：“基于线粒体 DNA 控制区域变异的日本黄鼠狼 Mustela itatsi（鼬科，食肉目）和日本岛屿上的西伯利亚黄鼠狼 M.sibirica 的分子系统发育地理学”林奈学会生物学杂志。