Genotoxicity of airborne particles from the urban area in Sapporo : Evaluation of the particulate samples collected over 22 year by bioassays

札幌市区空气中颗粒物的遗传毒性：通过生物测定法对 22 年来收集的颗粒物样本进行评估

• 批准号: 09557033
• 负责人: SATOH Hiroshi
• 金额: $ 8.32万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557033/
• 关键词: Urban airborne particulate; Nitroarene; Polynulear Aromatic Hydrocarbon; Genotoxicity; Ame test; Mouse lymphoma mutation assay (MLA); In vitro micronucleus test; Nitroarene metabolic enzyme deficient strain; Ames test; YG7126; 7130株; L5178Y細胞 /

To investigate genotoxicity of airborne particles, the airborne particulate samples collected in Sapporo, the most northern large city in Hokkaido, Japan, during the period of 24 years from 1974 were analyzed. The results obtained are followings :1. Concentrations of carcinogenic substances and mutagenicity assayed by Ames test.Concentrations of polycyclic aromatic hydrocarbons (PAHs) markedly decreased during the observation period of 24 years, while concentrations of nitroarenes did not. Mutagenic activities assayed by TA98 and TA100 strains did only slightly decrease. These results indicates that concentrations of PAHs is not an appropriate indicator for the atmospheric risk of lung cancer and further the risk to human health did not significantly decrease over the observation period in terms of mutagenicity in airborne particles.2. Mutanenicity assayed by mouse lymphoma cells.Mutagenicity assay using the L5178Y cell, a cell line of mouse lymphoma cells, efficiently detected mutatio … More ns and chromosomal aberration induced by the airborne particulate samples. The indirect mutagenic activities (+S9) clearly decreased over the period, while direct mutagenic activities (-S9) did not.3. Mutagenicity assayed by in vitro micronucleus test.It was shown that mutagenicity of airborne particles is possibly assayed by in vitro micronucleus test using Chinese hamster lung fibroblast cells. The incidences of induced micronucleus were rather stable for +S9 test system over the observation period. For the -S9 test system, the incidences were elevated in early 1980s with small fluctuations for other years.4. Development of new test strains with metabolic deficits.By gene disruption, new test strains for Ames assay, YG7128 and YG7132 with completely and partially inhibited nitroreductase activities, were established. YG7126 and YG7130, O-acetyltransferase deficient strains, were also established. These strains effectively detect nitroarenes and it was shown that contribution by nitroarenes was considerable among the total mutagenic activities in the airborne particulate samples. Less

为了研究大气颗粒物的遗传毒性，分析了从1974年开始的24年间在日本北海道最北方大城市札幌采集的大气颗粒物样品。主要研究结果如下：1.用艾姆斯试验测定致癌物质和致突变性，在24年的观察期内，多环芳烃（PAHs）的浓度明显下降，而硝基芳烃的浓度没有明显变化。TA 98和TA 100菌株的致突变活性仅略有降低。这些结果表明，多环芳烃的浓度不是一个合适的指标，肺癌的大气风险和进一步的人类健康的风险并没有显着降低在观察期内在空气颗粒物的致突变性.小鼠淋巴瘤细胞致突变性试验使用L5178 Y细胞（一种小鼠淋巴瘤细胞系）进行的致突变性试验有效地检测了突变。 ...更多信息 NS和染色体畸变。间接致突变活性（+S9）在此期间明显下降，而直接致突变活性（-S9）则没有下降.用体外微核试验测定致突变性用中国仓鼠肺成纤维细胞体外微核试验测定空气颗粒物的致突变性。在观察期内，+S9试验系统的诱导微核发生率相当稳定。对于-S9检测系统，发生率在20世纪80年代初升高，其他年份波动较小。代谢缺陷型试验菌株的选育通过基因敲除，建立了硝基还原酶活性完全和部分被抑制的艾姆斯试验菌株YG 7128和YG 7132。还建立了O-乙酰转移酶缺陷型菌株YG 7126和YG 7130。这些菌株有效地检测硝基芳烃，它表明，硝基芳烃的贡献是相当大的总致突变活性的空气颗粒物样品。少

项目成果

清水英佑他: "培養細胞を用いた小核試験による大気汚染物質の検討"日本環境変異原学会第26回大会. (1997)

Eisuke Shimizu 等人：“使用培养细胞进行微核测试的空气污染物研究”，日本环境诱变剂学会第 26 届年会（1997 年）。

Matsumoto Y.et al.: "Long-term trends of particulate mutagenic activity in the atmosphere of Sapporo"Environmental Science and Technology. 32. 2665-2671 (1998)

Matsumoto Y.等人：“札幌大气中颗粒物致突变活性的长期趋势”环境科学与技术。

Matsumoto, Y., S. Sakai, T. Kato, T. Nakajima and H. Satoh: "Long-term trends of particulate mutagenic activity in the atmosphere of Sapporo. 1. Determination of mutagenic activity by the conventional tester strains TA98 and TA100 during an 18-year period

Matsumoto, Y.、S. Sakai、T. Kato、T. Nakajima 和 H. Satoh：“札幌大气中颗粒物诱变活性的长期趋势。1. 通过常规测试菌株 TA98 和 TA100 测定诱变活性

Y.Matsumoto et al.: "Long-Term trends of particulate mutagenic activity in the atmosphere of sappro;part 1" Environmental Science and Technology. 32(18). 2665-2671 (1998)

Y.Matsumoto 等人：“萨普罗大气中颗粒诱变活性的长期趋势；第 1 部分”环境科学与技术。

松本寛他: "変異原性から見た過去四半世紀における札幌市大気粉塵の質的変遷"第9回環境化学討論会. (発表予定). (2000)

Hiroshi Matsumoto 等人：“从致突变性的角度看过去四分之一世纪札幌大气尘埃的质变”第九届环境化学研讨会（预定报告）（2000 年）。