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Elucidation of the mechanism of sex differentiation and its application to effective reproduction of animal resources

性别分化机制的阐明及其在动物资源有效繁殖中的应用

基本信息

批准号：
09558097
负责人：
MOROHASHI Ken-ichirou
金额：
$ 7.74万
依托单位：
09558097
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09558097/
关键词：
Ad4BP SF-1 sex differentiation gonad Dax- 1 Medaka frog chicken

项目摘要

The present research was performed to elucidate a mechanism underlying sex differentiation of the gonads and to apply it to effective reproduction of animal resources.Functions and expressions of transcription factors implicated in the gonadal development were mainly analyzed.In addition, analyses extending to other animals from mammalian species helped us to clarified the mechanism common to vertebrate as well as specific for animal species.Actually cDNAs and genes encoding Ad4BP/SF-1 were cloned from Medaka (fish) by Dr.Nagahama, from a frog (amphibian) by Dr.Nakamura, and from a chicken (bird) by Dr.Kudo.By using these DNAs, extensive efforts were made to elucidate the regulation of the Ad4BP/SF-1 and Dax-1 gene expression.As the results, it was clarified that the Dax-1 gene is positively regulated by the function of Ad4BP/SF-1, which probably constitutes a cascade of gene regulation functioning in the gonadal development.Analyses were also performed during the developmental process from an adreno-gonadal primordium to either an adrenal or gonadal primordium.Based on these observations, it was strongly suggested that Ad4BP/SF-1, originally identified as the transcription factor essential for differentiation of the mammalian gonads, play similar roles among other animal species.Although the fine mechanism of the sex differentiation has remained to be elucidated, the present study gives important cues for further investigation.

本研究旨在阐明性腺性别分化的机制，并将其应用于动物资源的有效繁殖。重点分析了与性腺发育有关的转录因子的功能和表达。此外，从哺乳动物扩展到其他动物的分析帮助我们澄清了脊椎动物共同以及动物特有的机制。实际上，编码Ad4BP/SF-1的cdna和基因是由nagahama博士从Medaka（鱼），nakamura博士从青蛙（两栖动物），kudo博士从鸡（鸟）中克隆出来的，通过这些dna，人们已经做了大量的工作来阐明Ad4BP/SF-1和Dax-1基因表达的调控。结果表明，Dax-1基因受到Ad4BP/SF-1功能的正向调控，可能构成了生殖腺发育过程中基因调控的级联机制。在从肾上腺-性腺原基到肾上腺或性腺原基的发育过程中也进行了分析。基于这些观察结果，我们强烈建议Ad4BP/SF-1，最初被确定为哺乳动物性腺分化所必需的转录因子，在其他动物物种中发挥类似的作用。虽然性别分化的精细机制仍有待阐明，但本研究为进一步研究提供了重要线索。