Development of Simulator for Vesicular Transport in Cells - Application to Study of Mechanism of Vesicular Transportation and Cell Locomotion.

细胞内囊泡运输模拟器的开发——在囊泡运输和细胞运动机制研究中的应用。

• 批准号: 09558111
• 负责人: KOSAWADA Tadashi
• 金额: $ 5.63万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09558111/
• 关键词: Vesicular Transport; Simulator; Bio-membrane; large Deformation Analysis; Analytical Method; Finite Element Method; Chained Vesicles; Outer Surrounding Cytoplasmic Membrane; 細胞内輸送; 膜小胞; 細胞移動; リポゾーム

A simulator for vesicular transport in cells was developed based on recent knowledge of molecular biology of the cell. Analytical method as well as finite element method were utilized to achieve the goal.1. Simulation of vesicular transport based on the analytical method(1) When the opening perimeter of a spherical membrane shell is unfolded with its perimeter kept parallel to its axis, there exists several points where the shapes and the tensions change significantly with slight changes of the opening radius. This is considered to be the bucking-like phenomena of the membrane with a spontaneous curvature.(2) In the repeating units of chained vesicles, it is found that the cylindrical tube shape and the hourglass shape are stable with respect to energy.(3) It is theoretically suggested that the chained vesicles and the cylindrical channel derived from the chained vesicles are some of the stable shapes with respect to energy and can potentially be formed and presented in the vascular en … More dothelial cell.(4) The computed shape suggests that the effects of in-plane shear elasticity and outer surrounding cytoplasmic membrane are significant and these should be taken into account in theoretical analysis.(5) The chained vesicle changes its shape dramatically even when the opening radius slightly increases from the starting shape. The jump-like phenomena of strain energy was often observed where each constriction disappears.2. Development of the specific finite element method for vesicular transport simulator by utilizing Mathematica(1) Formulation of the finite element method was developed based on elastic potential energy function. The requirement of constant surface area of the membrane was satisfied by implementing additional surface pressure.(2) The requirement of constant surface area of the membrane has significant effect on deformed shape of the vesicle.(3) Mooney-Rivlin type strain energy density function, which was developed for rubber red cell model, was utilized. The computed cortical tension was given as 0.003-0.18 dyn/cm. This results support the know experimental data of 0.03 dyn/cm. Less

基于细胞分子生物学的最新知识，开发了一种模拟细胞中囊泡运输的模拟器。采用解析法和有限元法相结合的方法来实现.基于解析法的囊泡输运模拟（1）当球形膜壳的开口周长展开并保持其周长与其轴平行时，存在几个点，在这些点处，形状和张力随开口半径的微小变化而显著变化。这被认为是具有自发曲率的膜的类屈曲现象。(2)在链状囊泡的重复单元中，发现圆柱形管形状和沙漏形状相对于能量是稳定的。(3)从理论上讲，链状囊泡和由链状囊泡衍生的圆柱形通道是相对于能量的稳定形状，并可能在血管内皮中形成和呈现。 ...更多信息 内皮细胞(4)计算的形状表明，面内剪切弹性和细胞质膜的外部周围的影响是显着的，这些应该考虑在理论分析。(5)即使当开口半径从起始形状稍微增加时，链式囊泡也会显著地改变其形状。在收缩消失处，应变能常出现类似跳跃的现象.基于Mathematica的囊泡输运模拟器专用有限元方法的开发（1）基于弹性势能函数建立了有限元方法的列式。通过施加附加表面压力，满足了膜表面积恒定的要求。(2)膜的恒定表面积的要求对囊泡的变形形状具有显著影响。(3)利用Mooney-Rivlin型应变能密度函数，该函数是为橡胶红细胞模型而开发的。计算出的皮质张力为0.003-0.18 dyn/cm。这一结果支持已知的0.03达因/厘米的实验数据。少

项目成果

Kosawada, T., Skalak, R., Schmid-Schoenbein, G.W.: "Chained Vesicles in Vascular Endothelial Cells"ASME Journal of Biomechanical Engineering. 121-5. 472-479 (1999)

Kosawada, T.、Skalak, R.、Schmid-Schoenbein, G.W.：“血管内皮细胞中的链状囊泡”ASME 生物力学工程杂志。

Kosawada, Skalak et al.: "Formation of Chained Vesicle on Vascular Endothelial Cell and Its Dynamic Aspects" Proc. International Conf. on New Frontiers in Biomechanical Engineering. 85-88 (1997)

Kosawada、Skalak 等人：“血管内皮细胞链状囊泡的形成及其动态方面”Proc。

Kosawada, Iijima, Kubota, Nakada, et al.: "A New Approach for Identification of Dynamic Properties of Mammalian Soft Tissues" Proc. International Conf. on New Frontiers in Biomechanical Engineering. 467-470 (1997)

Kosawada、Iijima、Kubota、Nakada 等人：“一种识别哺乳动物软组织动态特性的新方法”Proc。

Kosawada T., Takeno T., Skalak R., Schmid-Schonbein G.W.: "Formation of Chained Vesicle on Vascular Endothelial Cell and Its Dynamic Aspects"Proceedings of the International Conference on New Frontiers in Biomechanical Engineering, Tokyo. 85-88 (1997)

Kosawada T.、Takeno T.、Skalak R.、Schmid-Schonbein G.W.：“血管内皮细胞链状囊泡的形成及其动态方面”生物力学工程新前沿国际会议论文集，东京。

Kosawada T.: "Biological Tissue and Cell Mechanics"Journal of the JSME. 101-No.957. 411-412 (1998)

Kosawada T.：《生物组织和细胞力学》JSME 杂志。