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Development of Dynamic Sensing System to Detect Environmental Stresses Induced in Living Cell. - Application to Diagnosis of Living Cell

开发动态传感系统来检测活细胞中诱发的环境压力。

基本信息

批准号：
12450093
负责人：
KOSAWADA Tadashi
金额：
$ 8.45万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

In this study, a novel method to detect the environmental stresses induced in cell-like minute object is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses induced in egg cells of killifish as well as hardening process of tissue-engineered 3D cultured cartilage. The results can be summarized as follows.(1) A novel method of sensing to detect the softness of sponge-like soft and cell-like minute object is specifically presented by using dynamic response of piezoelectric vibrator. Experimental studies conducted on silicone rubber blocks, whose Shore hardness, Hs are known as 5-80, have shown the sensor's capability to classify the softness of sponge-like soft object.(2) With the aid of this sensing system, it is found that the living egg cells of killifish reveal their specific responses to the environments such as changes of temperature, concentration, pH, environmental hormone, etc. of surrounding solution or sucking pressure applied to the outside surface of the cell. Thus, the present sensor system has enough capability to monitor the environmental stresses induced in living cells.(3) The hardening process of tissue-engineered three dimensionally (3D) cultured cartilage has been studied with respect to culture periods. Dynamic properties as well as biochemical composition were examined. It is found that the present study has a possibility to provide a useful information to decide whether the cultured cartilage has enough hardness for transplantation treatment of articular cartilage damage.

本文提出了一种利用压电振动器的动态响应来检测细胞状微小物体中环境应力的新方法。实验研究表明，该方法能够检测鳉鱼卵细胞诱导的环境应力变化以及组织工程三维培养软骨的硬化过程。结果可以总结如下。(1)提出了一种利用压电振子的动态响应检测海绵状软质和细胞状微小物体柔软度的新方法。对硅橡胶块进行的实验研究表明，该传感器能够对海绵状柔软物体的柔软度进行分类，硅橡胶块的邵氏硬度Hs为5-80。(2)利用该传感系统，可以发现活的鳉鱼卵细胞对周围溶液的温度、浓度、pH值、环境激素等变化或细胞外表面施加的吸吮压力等环境的特定反应。因此，目前的传感器系统有足够的能力监测在活细胞中诱导的环境应力。(3)对组织工程三维培养软骨的硬化过程进行了不同培养周期的研究。对其动态性能和生化组成进行了检测。发现本研究有可能为确定培养软骨是否具有足够的硬度用于关节软骨损伤的移植治疗提供有用的信息。