Detection of Environmental Stresses and Diagnosis System for Living Cells Based on Piezo Micro Vibration Sensor

本文提出了一种利用压电振子的动态响应来检测活细胞内环境应力的新方法。实验研究表明，它能够检测自然或人工诱导的环境应力在鱼卵细胞以及培养的人成骨细胞中的变化。结果可归纳如下。(1)在卵细胞对草铵膦（典型除草剂的主要物质）的反应中，借助于该传感系统，在100ppm的情况下，由于急性毒性，结果在注射草铵膦后立即上升，而10ppm的情况下，结果可能由于“低浓度效应”而下降。这些现象被认为反映了细胞对周围环境的初始适应过程。(2)结果表明，受损卵细胞的表面膜比正常卵细胞的表面膜硬，而刺激卵细胞的表面膜比受损卵细胞的表面膜硬。受损细胞和动态刺激的细胞都被认为在48小时左右恢复正常。还观察到对动态刺激的响应取决于频率。(3)建立了体外培养的人成骨细胞动态刺激及反应检测的实验方法。观察到人成骨细胞不仅具有对动态刺激的检测能力，而且具有向邻近细胞传递信息的能力。

In this study, a novel method to detect the environmental stresses induced in living cell is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses naturally or artificially induced in egg cells of killifish as well as cultured human osteoblast. The results can be summarized as follows.(1) In the egg cell response to Glufosinate (main substance of typical weed killer), with the aid of this sensing system, in the 100ppm case the results are going up just after the Glufosinate injection due to acute toxicity, while 10ppm case the results are going down probably due to "low concentration effect". These phenomena are considered to reflect initial adaptation process of the cell to the surrounding environments.(2) It is found that surface membrane of the damaged egg cell is harder than the normal cell, while the stimulated egg cell is harder than the damaged cell. Both the damaged cell and the dynamically stimulated cell are considered to be normalized with 48 hours or so. It is also observed that the response to dynamic stimulation depends on frequency.(3) Experimental method was developed for dynamic stimulation and detection of its response for cultured human osteoblast. It is observed that not only human osteoblast's detection ability against dynamic stimulations but also its information transfer ability to neighboring cells.