Detection of Environmental Stresses and Diagnosis System for Living Cells Based on Piezo Micro Vibration Sensor

本文提出了一种利用压电振子的动态响应来检测活细胞内环境应力的新方法。实验研究表明，该方法能检测自然或人工诱导的环境应激变化，并能检测培养的人成骨细胞和鳉鱼卵细胞。结果可以总结如下。(1)在卵细胞对草铵膦（典型除草剂的主要物质）的反应中，在该传感系统的帮助下，在100ppm的情况下，由于草铵膦注射后的急性毒性，结果呈上升趋势，而在10ppm的情况下，结果可能由于“低浓度效应”而下降。这些现象被认为反映了细胞对周围环境的初始适应过程。(2)发现受损卵细胞的表面膜比正常细胞硬，而受刺激的卵细胞比受损细胞硬。受损细胞和受动态刺激的细胞在48小时左右被认为是正常的。还观察到，对动态刺激的响应取决于频率。(3)建立了对培养的人成骨细胞进行动态刺激并检测其反应的实验方法。观察到人成骨细胞不仅具有对动态刺激的检测能力，而且具有向邻近细胞传递信息的能力。

In this study, a novel method to detect the environmental stresses induced in living cell is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses naturally or artificially induced in egg cells of killifish as well as cultured human osteoblast. The results can be summarized as follows.(1) In the egg cell response to Glufosinate (main substance of typical weed killer), with the aid of this sensing system, in the 100ppm case the results are going up just after the Glufosinate injection due to acute toxicity, while 10ppm case the results are going down probably due to "low concentration effect". These phenomena are considered to reflect initial adaptation process of the cell to the surrounding environments.(2) It is found that surface membrane of the damaged egg cell is harder than the normal cell, while the stimulated egg cell is harder than the damaged cell. Both the damaged cell and the dynamically stimulated cell are considered to be normalized with 48 hours or so. It is also observed that the response to dynamic stimulation depends on frequency.(3) Experimental method was developed for dynamic stimulation and detection of its response for cultured human osteoblast. It is observed that not only human osteoblast's detection ability against dynamic stimulations but also its information transfer ability to neighboring cells.