Effects of nitric oxide in ion channel in hypertensive pulmonary vascular smooth muscle cells.

我们建立了分离肺血管平滑肌细胞的方法，以研究高血压肺血管平滑肌细胞的离子通道。培养的平滑肌细胞表型发生改变，可能存在成纤维细胞等其他类型细胞的污染。因此，我们估计分离的肺动脉血管平滑肌比培养的细胞更适合。在含胶原酶20 mg的无钙台氏液中分离肺动脉平滑肌细胞。细胞悬浮液通过尼龙网。在慢性缺氧性肺动脉高压模型上，NO可使KCL引起的去极化收缩作用减弱，但细胞内钙离子浓度变化不大。NO可能是由于肺动脉平滑肌细胞对钙的敏感性所致，尤其是在肺动脉高压时。

We established the method of isolation of pulmonary vascular smooth muscle cells to investigate the ion channel of hypertensive pulmonary vascular smooth muscle cell. Cultured smooth muscle cells had changes in phenotype and there might be comtamination of other types of cell such as fibroblast. So, we estimated that isolated pulmonary artery vascular smooth muscle was more suitable than cultured cells. Isolation of pulmonary arterial smooth muscle cells was performed in clcium free Tyrode solutioncontaining 20mg collagenase. The cell suspended solution was passed through the nylon mesh. In chronic hypoxia-induced pulmonary hypertensive pulmonary arteries, KCL-induced depolarization-induced-contractions were reduced by NO doner with small changes in intracellular calcium concentration. NO might after calcium sensitivity of pulmonary arterial smooth muscle cells especially in pulmonary hypertension.