Effects of nitric oxide in ion channel in hypertensive pulmonary vascular smooth muscle cells.

一氧化氮对高血压肺血管平滑肌细胞离子通道的影响。

• 批准号: 09470326
• 负责人: MARUYAMA Kazuo
• 金额: $ 6.27万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470326/
• 关键词: nitric oxide; pulmonary hypertension

We established the method of isolation of pulmonary vascular smooth muscle cells to investigate the ion channel of hypertensive pulmonary vascular smooth muscle cell. Cultured smooth muscle cells had changes in phenotype and there might be comtamination of other types of cell such as fibroblast. So, we estimated that isolated pulmonary artery vascular smooth muscle was more suitable than cultured cells. Isolation of pulmonary arterial smooth muscle cells was performed in clcium free Tyrode solutioncontaining 20mg collagenase. The cell suspended solution was passed through the nylon mesh. In chronic hypoxia-induced pulmonary hypertensive pulmonary arteries, KCL-induced depolarization-induced-contractions were reduced by NO doner with small changes in intracellular calcium concentration. NO might after calcium sensitivity of pulmonary arterial smooth muscle cells especially in pulmonary hypertension.

本实验建立了肺血管平滑肌细胞分离方法，以研究高血压肺血管平滑肌细胞离子通道。培养的平滑肌细胞表型发生变化，可能存在其他类型细胞（如成纤维细胞）的污染。因此，我们认为分离的肺动脉血管平滑肌细胞比培养的细胞更适合。在含有20 mg胶原酶的无钙台氏液中分离肺动脉平滑肌细胞。使细胞悬浮溶液通过尼龙网。在慢性缺氧性肺动脉高压肺动脉中，KCl诱导的去极化收缩被NO供体减少，细胞内钙浓度变化不大。NO可能参与了肺动脉平滑肌细胞钙敏感性的调节，尤其是在肺动脉高压时.

项目成果

Yoshida M.et al.: "The effect of low-dose inhalation of nitric oxide in patients with pulmonary fibrosis" Eur Respir J. 10. 2051-2054 (1997)

Yoshida M.et al.：“低剂量吸入一氧化氮对肺纤维化患者的影响”Eur Respir J. 10. 2051-2054 (1997)

Maruyama J.et al.: "Acetylcholine-induced endothelium-derived contracting factor in hypovic pulmonary hypertengive rats" J.Appl.Physiol.86(in press). (1999)

Maruyama J.等人：“缺氧性肺动脉高压大鼠中乙酰胆碱诱导的内皮衍生收缩因子”J.Appl.Physiol.86（印刷中）。

Mitani Y., Maruyama J., Maruyama K., Sakurai M.: "Exercise training does not alter acetylcholine-induced responses in isolated pulmonary artery from rat." Eur Respir J. (in press). (1999)

Mitani Y.、Maruyama J.、Maruyama K.、Sakurai M.：“运动训练不会改变大鼠离体肺动脉中乙酰胆碱诱导的反应。”

Mitani Y et、al.: "Prolonged Administration of L-Arginine Ameliorates Chronic Pulmonary Hypertension and pulmonary vascular Remodeling in rats" Circulation. 96. 689-697 (1997)

Mitani Y 等人：“L-精氨酸的长期给药可改善大鼠的慢性肺动脉高压和肺血管重塑”循环。 96. 689-697 (1997)

Yoshida M.et al: "The effect of low-dose inhalation of nitric oxide in patients with pulmonary fibrosis" Eur Respir J. 10. 2051-5054 (1997)

Yoshida M.et al：“低剂量吸入一氧化氮对肺纤维化患者的影响”Eur Respir J. 10. 2051-5054 (1997)