Ultrasound cancer therapy in next-generation with liposomal nanobubbles having both functions of diagnostics and therapeutics

新合成了一种用于高PFC的F-DPPC作为BL的壳层。合成了一种新型的包衣材料--氟化磷脂(F-DPPC)，以期保留PFC。制备了C5F12：C6F14=1：1(质量比)混合气体的RGD-BL(DSPC:F-DPPC:DSPE-PEG2000:DSPE-PEG3000-Mal=44:50:5:1(摩尔比)，直径约500 nm。RGD-BL与诊断性超声相结合，显示实体瘤的血栓和新生血管。RGD-BL与治疗性US的结合，可通过引入pCMV-IL12进行基因治疗，并结合瘤内温升进行热疗。因此，RGD-BL和US的结合可用于治疗和诊断相结合的超声治疗系统，这是下一代。

F-DPPC for high PFC was newly synthesized as shell of BL. Fluorinated phospholipid (F-DPPC) was synthesized as a new outer shell material of BL for the purpose of retention of PFC. RGD-BL (DSPC:F-DPPC:DSPE-PEG2000:DSPE-PEG3000-Mal=44:50:5:1 (molar ratio)) encapsulating mixed gas of C5F12:C6F14=1:1 (weight ratio), was prepared with about 500 nm in diameter. The combination of RGD-BL and diagnostic US make the image of the thrombus and neovasculature of solid tumor. The combination of RGD-BL and therapeutic US can be use for gene therapy by introduction of pCMV-IL12 and hyperthermia treatment with intratumoral temperature rise. Thus, the combination of RGD-BL and US may be used for ultrasound theranostics system with therapeutic and diagnostic, which is a next-generation.