Structural and functional analysis of signalling complexes formed by the ethylene receptor ETR1 in response to signal perception

乙烯受体 ETR1 响应信号感知形成的信号复合物的结构和功能分析

• 批准号: 92792267
• 负责人: Professor Dr. Georg Groth
• 金额: --
• 依托单位: Institut für Biochemische Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/92792267?language=en
• 关键词: Structural; functional; analysis; signalling; complexes

The plant hormone ethylene influences many processes of plant growth and development and is known as the hormone which initiates fruit ripening. Reverse genetics has identified a family of membrane intrinsic proteins (ETR1, ERS1, ETR2, ERS2, E1N4) similar to bacterial two-component histidine kinases as receptors of the gaseous hormone, and also several downstream components of the ethylene signalling cascade. These data suggest that the receptors have different operation modes for the signalling of the plant hormone and interact with the soluble Ser-Thr kinase CTR1, but also with the histidine-containing transfer protein AHP1. In order to understand this interaction with alternative signalling partners on a more quantitative level and to recognize the processes taking place at the beginning of the signalling cascade more precisely our project is determined to resolve complex formation and stability as well as high-resolution molecular structure of the ETR1-AHP1 and the ETR1-CTR1-complex by a biochemical and biophysical approach using fluorescence spectroscopy, protein crystallisation and X-ray diffraction studies.

植物激素乙烯影响植物生长和发育的许多过程，被认为是启动果实成熟的激素。反向遗传学研究发现，与细菌双组分组氨酸激酶类似的一系列膜内源蛋白(ETR1、ERS1、ETR2、ERS2、E1N4)是气态激素的受体，也是乙烯信号级联反应的下游成分。这些数据表明，这些受体不仅与可溶性丝氨酸-苏氨酸激酶CTR1相互作用，而且与含有组氨酸的转运蛋白AHP1相互作用，具有不同的植物激素信号传导模式。为了在更定量的水平上了解与替代信号伙伴的这种相互作用，并更准确地认识信号级联开始时发生的过程，我们的项目决心通过荧光光谱、蛋白质结晶和X射线衍射研究，通过生化和生物物理方法解决ETR1-AHP1和ETR1-CTR1-复合体的形成和稳定性以及高分辨率分子结构。