权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

International Collaboration on Color Vision in Arthropods

节肢动物色觉国际合作

基本信息

批准号：
10044090
负责人：
ARIKAWA Kentaro
金额：
$ 4.74万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

The specific aim of this international research project was to understand the basic design of color vision system of butterflies. Below are the results obtained from this project.General appearance of ommatidial heterogeneity in butterfliesWe have discovered that the ommatidia of the Japanese yellow swallowtail butterfly, Papilio xuthus are spectrally heterogeneous. We identified at least three types of ommatidia, which are randomly distributed over the retina. The random distribution of different types of ommatidia may be related to their color vision. We therefore observed the tapetal reflection of more than 50 butterfly species, occurring mainly in Taiwan, by using epi-illumination metal microscope. In all species observed, the color and/or intensity of tapetal reflection appeared to be heterogeneous among ommatidia. This clearly indicates that the ommatidial heterogeneity and their random distribution is a general property of butterfly compound eyes. Based on the data obtained, we … More carefully selected several species with which more detailed research will be carried out, and we initiated some pilot experiments.Physiological and molecular biological study of the spectral sensitivities of Papilio photoreceptorIn the Papilio retina, we previously identified a violet receptor whose spectral sensitivity function is considerably narrower compared to other spectral receptors. Here we demonstrated that the violet receptors reside always in the ommatidia emitting strong fluorescence under UV epi-illumination. The fluorescence is due to 3OH hydroxyretinol, which is acting as an UV-absorbing filter. The violet receptors were also shown to contain UV-absorbing visual pigment. This gives a molecular biological support to our previous hypothesis that the violet receptors are produced by UV absorbing visual pigment filtered by the 3-OH hydroxyretinol. Also, we found a novel type of spectral receptor whose spectral sensitivity has a very broad (230 nm in half-bandwidth) sensitivity spectrum in the visible region and completely lacks the sensitivity in the UV wavelength region. Because of the abnormally-broad sensitivity, we termed the cell as A-cell. The spectral origin of the A-cell was also determined. Less

本次国际研究项目的具体目的是了解蝴蝶颜色视觉系统的基本设计。以下是本项目所获得的结果。蝴蝶小眼异质性的一般表现我们发现，日本黄燕尾蝴蝶的小眼具有光谱异质性。我们确定了至少三种类型的小眼，它们随机分布在视网膜上。不同类型小眼的随机分布可能与其色觉有关。因此，我们利用表照金属显微镜观察了50多种蝴蝶的绒被反射，这些蝴蝶主要发生在台湾。在观察到的所有物种中，绒被反射的颜色和/或强度在小眼之间似乎是不同的。这清楚地表明，小眼的不均一性及其随机性分布是蝴蝶复眼的普遍特征。基于所获得的数据，我们…更仔细地选择了几种将进行更详细研究的物种，并启动了一些初步实验。Papilio光感受器的光谱敏感性的生理和分子生物学研究在Papilio视网膜中，我们先前发现了一种紫色感受器，其光谱敏感性功能比其他光谱感受器要窄得多。在此，我们证明了紫外光照射下，紫罗兰色受体始终存在于小眼内，发出强烈的荧光。荧光是由于3OH羟基视黄醇，它起到了紫外线吸收滤光器的作用。紫色受体也被证明含有紫外线吸收的视觉色素。这为我们之前的假设提供了分子生物学支持，即紫色受体是由3-羟基视黄醇过滤的紫外光吸收的视觉色素产生的。此外，我们还发现了一种新型的光谱感受器，其光谱灵敏度在可见光区域具有非常宽的(230 nm半带宽)灵敏度光谱，而在UV波长区完全没有灵敏度。由于异常广泛的敏感性，我们将该细胞称为A细胞。还确定了A细胞的光谱来源。较少