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Neural mechanism of color information processing in insect visual system

昆虫视觉系统颜色信息处理的神经机制

基本信息

批准号：
10440250
负责人：
ARIKAWA Kentaro
金额：
$ 8.32万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

・By measuring feeding response elicited by monochromatic stimuli, we determined the action spectrum of the foraging behavior in Papilio xuthus. We found that ultraviolet, blue, and red wavelength regions have positive effect, whereas green wavelength region has negative effect. The action spectra measured by the visiting response and the proboscis extension response differ each other, indicating that these responses are controlled by different neural mechanism.・We found that 3-OH retinol is the origin of the ommatidial fluorescence in Papilio. We here found and identified a novel retinol-binding protein in the Papilio eye. We first analyzed the amino acid sequence of the protein in part, which was then used to design oligo nucleotide primers for RT-PCR.In the end we determined the nucleotide sequence of the full-length mRNA encoding the protein.・We previously cloned 5 different cDNAs each encoding different visual pigment opsin. By in situ hybridization method, we determined the opsin mRNA expressed in the basal photoreceptor R9. It appeared that R9s in all ommatidia express mRNAs two green-absorbing opsins, Px-L1 and Px-L2, simultaneously. By having this particular piece of data, the cellular organization of Papilio retina was understood almost completely.・We established the method for intracellular recording and dye injection of the large monopolar cells (LMC), the second order visual interneurons, in the Papilio lamina. We further analyzed the structure of some stained LMCs by using confocal laser scanning microscopy.

·通过测量单色刺激引起的摄食反应，确定了黄裳凤蝶（Papilio xuthus）觅食行为的动作谱。我们发现，紫外，蓝色和红色波长区域有积极的影响，而绿色波长区域有负面影响。由拜访反应和长鼻伸展反应测量的动作谱彼此不同，表明这些反应由不同的神经机制控制。·我们发现凤蝶小眼荧光的来源是3-OH视黄醇。我们在凤蝶眼睛中发现并鉴定了一种新的视黄醇结合蛋白。我们首先对该蛋白的部分氨基酸序列进行了分析，然后根据分析结果设计寡核苷酸引物进行RT-PCR，最后确定了编码该蛋白的全长mRNA的核苷酸序列。我们以前克隆了5个不同的cDNA，每个编码不同的视色素视蛋白。通过原位杂交方法，我们确定视蛋白mRNA表达的基础感光细胞R9。似乎所有小眼中的R9同时表达两种绿色吸收视蛋白Px-L1和Px-L2的mRNA。通过获得这一特定数据，几乎完全了解了凤蝶视网膜的细胞组织。建立了蝶形层内二级视中间神经元大单极细胞（LMC）的细胞内记录和染料注射方法。我们利用共聚焦激光扫描显微镜进一步分析了一些染色的LMCs的结构。