Embryo transfer/In vitro fertilization of bovine and buffalo

牛和水牛的胚胎移植/体外受精

• 批准号: 10044211
• 负责人: SUZUKI Tatsuyuki
• 金额: $ 3.2万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044211/
• 关键词: bovine; buffalo; superovulation; recover of embryos; in vitro fertilization; simple portable CO2 incubator; CR1aa; CR2aa; somatotropin; blastocyst; ガス培養器; 陰圧; 発生; 牛; イージーブリード

Three of bovine and four of buffalo cows were injected by FSH for superovulation treatment. On day 7, embryos were recovered from these treated cows by non-surgical methods. As a results, number of transferable embryos per cows were obtained 6 out of 7.3 for bovine cows and 1.8 out of 2.3 for buffalo cows, respectively. However, number of transferable embryos were extremely reduced after re-treatment of superovulation (1.3 out of 3.7 for bovine and 0.5 out of 1.5 for buffalo cows, respectively).Buffalo and Bovine cows oocytes were collected for IVM, IVF and IVC. A portable CO2 incubator and CR1aa or CR2aa medium in the presence of somatotropin(GH) were used for in vitro production of embryos. From the present data, 200ng/ml GH in CR1aa medium seems to provide the optimum in vitro production of bovine embryos, although such high a high concentration of GH had no influence in CR2aa medium, may play a key role in subsequent development of embryos in vitro. In fact, we obtained the 40.2% of transferable embryos using CR2aa medium for bovine and buffalo. Also, our improved simple portable incubator can be used successfully for in vitro production of bovine embryos and could be applicable at the field level.

用FSH对3头黄牛和4头布法罗母牛进行超数排卵处理。在第7天，通过非手术方法从这些给药奶牛中回收胚胎。结果，每头母牛的可移植胚胎数分别为6/7.3（普通母牛）和1.8/2.3（布法罗母牛）。然而，再次超排处理后，可移植胚胎数显著减少（牛为1.3/3.7，水牛为0.5/1.5）。收集布法罗和牛卵母细胞进行IVM、IVF和IVC。采用便携式CO2培养箱和CR 1aa或CR2 aa培养基，在生长激素（GH）的存在下，用于体外生产胚胎。从目前的数据来看，200 ng/ml的GH在CR 1aa培养基中似乎提供了最佳的体外胚胎生产，虽然如此高的GH浓度在CR2 aa培养基中没有影响，但可能在随后的体外胚胎发育中起关键作用。事实上，我们获得了40.2%的可移植胚胎使用CR 2aa培养基牛和布法罗。此外，我们改进的简易便携式培养箱可以成功地用于牛胚胎的体外生产，并可应用于现场水平。

项目成果

Suzuki T. and others.: "n vitro Procduction of bovine embryos inthe presence of growth hormone in CR1aa or CR2aa medium using an improved portable CO2 incubator"Theriogenology. 53. 301-301 (2000)

Suzuki T. 等人：“使用改进的便携式 CO2 培养箱在 CR1aa 或 CR2aa 培养基中存在生长激素的情况下体外生产牛胚胎”Theriogenology。

ABDOON ASS and others: "Factors affecting number of surface antral follicles, quantity and quality of oocytes in egyptian buffalo ovaries."Anim. Reprod. Sci.. (in press).

ABDOON ASS 等人：“影响埃及水牛卵巢表面窦卵泡数量、卵母细胞数量和质量的因素。”Anim。

Suzuki T and others: "Development of a simple,portable carbon dioxide incubator for in vitro production of bovine embryos"Anim.Reprod.Sci.. 54. 149-157 (1999)

Suzuki T 等人：“用于牛胚胎体外生产的简单便携式二氧化碳培养箱的开发”Anim.Reprod.Sci.. 54. 149-157 (1999)

DONG YJ and others: "production of bovine IVF embryos using portable carbon dioxide incubator-Effect of negative air pressure on embryonic development."Jpn j Fertil Steril. 45. 31-36 (2000)

董YJ等：“利用便携式二氧化碳培养箱生产牛IVF胚胎——负气压对胚胎发育的影响”，Jpn j Fertil Steril。

SUZUKI T and others: "In vitro production of bovine embryos in the presence of growth hormone in CR1aa or CR2aa medium using an improved portable CO2 incubator."Theriogenology. 53. 301 (2000)

SUZUKI T 等人：“在 CR1aa 或 CR2aa 培养基中存在生长激素的情况下，使用改进的便携式 CO2 培养箱体外生产牛胚胎。”动物发生学。