Impact of electronic nicotine vapor on mouse mesolimbic CRFR1 circuitry and motivated behavior
电子尼古丁蒸气对小鼠中脑边缘 CRFR1 电路和动机行为的影响
基本信息
- 批准号:10151988
- 负责人:
- 金额:$ 3.93万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAdolescentAffectAnimalsAnxietyBehaviorBindingBrainCRF receptor type 1ChronicCigaretteCorticotropin-Releasing HormoneDataDevelopmentDopamineElectronic cigaretteElectrophysiology (science)Exposure toFemaleFoundationsGlutamatesGreen Fluorescent ProteinsImmunohistochemistryIn Situ HybridizationLeadMeasuresMembraneMusNeuronal PlasticityNeuronsNeuropeptidesNicotineNicotine DependenceNucleus AccumbensOralPathway interactionsPeripheralPharmaceutical PreparationsPlayPoliciesPopulationPropertyRegulationReinforcement ScheduleRewardsRodentRoleSelf AdministrationSerumSignal TransductionSliceStressSystemTestingTransgenic MiceVentral Tegmental AreaViraladdictionbrain behaviorcell typecellular targetingcombustible cigarettedopaminergic neuronexperimental studymalemotivated behaviormouse modelneuromechanismnicotine usenicotine vaporpromoterreward circuitrytargeted treatmenttransmission processvapingvapor
项目摘要
PROJECT SUMMARY/ABSTRACT
Nicotine is a highly addictive substance found in cigarettes as well as in electronic nicotine vapor products.
More recently, nicotine delivered through electronic vapor systems have grown in popularity, especially in the
adolescent population. Although these products are often marketed as safer alternatives, the effects of
electronically delivered nicotine vapor exposure on the brain and behavior, remain understudied. Nicotine
activates the brain reward pathway which mainly consists of dopaminergic neurons in the ventral tegmental
area (VTA) that sends projections and release dopamine (DA) into the nucleus accumbens (NAc). The VTA is
a heterogenous neuron population, including dopaminergic, glutamatergic, and GABAergic neurons that can
interact to differentially modulate reward signaling. Nicotine also plays an important role in modulating stress
and anxiety behaviors which in turn can exacerbate nicotine addiction. A principal component of central and
peripheral stress regulation is the corticotropin-releasing factor (CRF) system. The CRF neuropeptide binds
primarily to corticotropin-releasing factor 1 receptors (CRFR1) in the brain which are expressed in VTA
neurons. However, the role of VTA CRFR1 neurons in the reward pathway and the impact of nicotine vapor
exposure on reward signaling, remain unclear. To investigate the VTA CRFR1 population and its role in
nicotine effects on the reward pathway, I will use a transgenic mouse model expressing green fluorescent
protein under the CRFR1 promoter (CRFR1-GFP). I will characterize the cell types, projections,
electrophysiological properties, and sensitivity to cellular nicotine application of VTA CRFR1 neurons to
establish foundational information in naïve male and female animals (Aim 1). Using a rodent electronic nicotine
vapor system, I will expose male and female mice acutely and chronically to nicotine vapor and examine the
changes in neuronal activity and electrophysiological properties of VTA CRFR1 neurons (Aim 2). Additionally, I
will examine motivated behavior using self-administration of electronic nicotine vapor in different reward
conditions (increasing effort or decreasing reward value, Aim3). My overarching hypothesis is that CRFR1
neurons in the VTA are involved in the mesolimbic reward circuitry and that exposure to chronic electronic
nicotine vapor will alter basal electrophysiological properties and sensitivity to cellular nicotine, leading to
maladaptive behaviors. Together, the proposed experiments will reveal how acute and chronic electronic
nicotine vapor exposure can alter a stress-sensitive component of the mesolimbic reward circuit and contribute
to maladaptive behaviors like drug self-administration. Understanding the mechanisms that integrate stress
and reward in the context of nicotine addiction can better inform policies that regulate the availability of nicotine
vapor products and potentially identify cellular targets for therapeutics.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ManHua Zhu其他文献
ManHua Zhu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ManHua Zhu', 18)}}的其他基金
Impact of electronic nicotine vapor on mouse mesolimbic CRFR1 circuitry and motivated behavior
电子尼古丁蒸气对小鼠中脑边缘 CRFR1 电路和动机行为的影响
- 批准号:
10618784 - 财政年份:2021
- 资助金额:
$ 3.93万 - 项目类别:
Impact of electronic nicotine vapor on mouse mesolimbic CRFR1 circuitry and motivated behavior
电子尼古丁蒸气对小鼠中脑边缘 CRFR1 电路和动机行为的影响
- 批准号:
10356049 - 财政年份:2021
- 资助金额:
$ 3.93万 - 项目类别:
相似海外基金
Understanding How Adolescent Bullying Experiences Affect Traumatic Stress,Sexual Health and STI Risk among Men Who Have Sex with Men (MSM)
了解青少年欺凌经历如何影响男男性行为者 (MSM) 的创伤性压力、性健康和性传播感染风险
- 批准号:
10553263 - 财政年份:2022
- 资助金额:
$ 3.93万 - 项目类别:
Understanding How Adolescent Bullying Experiences Affect Traumatic Stress,Sexual Health and STI Risk among Men Who Have Sex with Men (MSM)
了解青少年欺凌经历如何影响男男性行为者 (MSM) 的创伤性压力、性健康和性传播感染风险
- 批准号:
10347813 - 财政年份:2022
- 资助金额:
$ 3.93万 - 项目类别:
Visuocortical Dynamics of Affect-Biased Attention in the Development of Adolescent Depression
青少年抑郁症发展过程中情感偏向注意力的视觉皮层动力学
- 批准号:
10380686 - 财政年份:2019
- 资助金额:
$ 3.93万 - 项目类别:
Visuocortical Dynamics of Affect-Biased Attention in the Development of Adolescent Depression
青少年抑郁症发展过程中情感偏向注意力的视觉皮层动力学
- 批准号:
9888437 - 财政年份:2019
- 资助金额:
$ 3.93万 - 项目类别:
Visuocortical Dynamics of Affect-Biased Attention in the Development of Adolescent Depression
青少年抑郁症发展过程中情感偏向注意力的视觉皮层动力学
- 批准号:
10597082 - 财政年份:2019
- 资助金额:
$ 3.93万 - 项目类别:
Targeting maladaptive responding to negative affect in adolescent cannabis users
针对青少年大麻使用者的负面影响的适应不良反应
- 批准号:
9371970 - 财政年份:2017
- 资助金额:
$ 3.93万 - 项目类别:
Childhood positive affect and anger as predictors of adolescent risky behavior
童年积极影响和愤怒是青少年危险行为的预测因素
- 批准号:
9139461 - 财政年份:2015
- 资助金额:
$ 3.93万 - 项目类别:
Do State Marijuana Policies Affect Adolescent Marijuana and Alcohol Use?
州大麻政策会影响青少年大麻和酒精的使用吗?
- 批准号:
8783159 - 财政年份:2014
- 资助金额:
$ 3.93万 - 项目类别:
Do State Marijuana Policies Affect Adolescent Marijuana and Alcohol Use?
州大麻政策会影响青少年大麻和酒精的使用吗?
- 批准号:
8853783 - 财政年份:2014
- 资助金额:
$ 3.93万 - 项目类别:
Assessment of Affect Instability in Adolescent Girls with BPD Features
具有 BPD 特征的青春期女孩的情绪不稳定评估
- 批准号:
8122499 - 财政年份:2011
- 资助金额:
$ 3.93万 - 项目类别:














{{item.name}}会员




