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Core B - Genomics Core

核心 B - 基因组学核心

基本信息

批准号：
10153658
负责人：
Steven M Wolinsky
金额：
$ 30万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10153658
关键词：
Affect Antiviral Agents Binding Bioinformatics CD94 Antigen Cells ChIP-seq Clinical Communicable Diseases Complex Data Data Set Dengue Dengue Infection Dengue Virus Disease Flavivirus Gene Expression Genes Genetic Markers Genomics Genotype Goals High-Throughput Nucleotide Sequencing Human Human Genetics Immune Immune System Diseases Immune response Immunity Immunology Infection Information Resources Innate Immune System Investigation MHC Class I Genes Massive Parallel Sequencing Measurement Measures Messenger RNA Methods Modeling Molecular Profiling Outcome Pathogenesis Pathway Analysis Pathway interactions Pattern Pharmacotherapy Population Predisposition RNA RNA Interference Receptor Gene Research Personnel Resources Risk Serotyping Severity of illness Signal Pathway Structure Systems Biology Technology Testing Transcript Vaccination Vaccines Variant Vertebral column Virulence Virus Virus Diseases Yellow fever virus antiviral immunity base biomarker identification cell type cohort env Gene Products high throughput screening histone modification immunological status knock-down knowledge base mosquito-borne response single-cell RNA sequencing transcription factor transcriptome sequencing vaccination outcome vaccine response

项目摘要

PROJECT SUMMARY The innate immune recognition of dengue virus infection triggers antiviral immune responses; however, there is little information about how the virus regulates these measures or how these responses affect dengue virus (DENV) disease pathogenesis and immunity. System biology approaches aimed at deciphering the complex interactions between DENV and host genes and pathways require accurate high-throughput measurements and comprehensive datasets and models. The purpose of this HIPC is to take advantage of recent advances in human immune profiling methods to characterize the early states of the human innate immune system following DENV infection and before and after vaccination against this infectious disease. The goal of the Genomics Core is to provide a central knowledge base and resource to define the human immune repertoire and develop predictors of disease and vaccine responses by using high throughput sequencing and bioinformatics that facilitate investigations of human immunity in well-characterized human cohorts. Other HIPC investigators will use this information to derive human immune profiles or signatures for disease in naturally acquired infection with known dengue immune status (Project 1), vaccine responsiveness and human challenge studies (Project 2), or DENV infection ex vivo with DENV strains of different virulence (Project 3). Network analysis of the human immune profiling data will identify pathways and genes for RNAi knockdown to determine how perturbations of the regulatory networks influence host responses during DENV infection. These studies are key to the overall goal of the HIPC to use high-throughput systems biology approaches to create molecular signatures that will define the human immune response profiles that correlate with the outcome of dengue virus infection or vaccination.

项目总结