Special Projects: Method Development and Method Validation

学科D：特别项目 分析轨道3：方法开发和方法验证 项目摘要/摘要： 佛罗里达州农业和消费者服务部(FDAC)食品安全局 实验室于2007年获得认证，并保持对ISO/IEC的A2LA认证 17025：A2LA证书2534.01、2534.02和2534.03中列出的具体测试 (可在www.A2LA.org上查阅)。该司有责任履行其职责或 支持功能遵循佛罗里达州法规的以下章节：500、502、583、586和 601.食品安全部负责确保公众获得安全、卫生和 适当地代表了食品供应。这是通过允许和检查以下各项来实现的 食品企业；食品和乳制品的检验和评价；以及 对各种食品和乳制品进行生物和化学实验室检测。 食品安全实验室分部正在申请这项名为： 实验室灵活资助模式(LFFM)，公告编号PAR-20-105，带 预计从2020年到2025年提供资金，以加强以下方面的能力和能力 微生物学和化学领域的方法开发和方法验证。这个 实验室目前由佛罗里达州微生物和化学FDA蕨类基金资助 计划，2015-2020(5U18FD005506-05)。DFS-生物微生物学计划一直是一个 自2004年蕨类微生物计划启动以来，积极资助实验室，并已 参与了FERN和FDA的微生物方法开发和方法验证。 DFS-Bio承担了大规模的方法开发项目，包括开发 产志贺毒素大肠杆菌(STEC)O157和非O157的实时荧光定量PCR检测方法 O157“六大”大肠埃希菌及卡氏环孢菌检测新方法的建立 在新鲜的草药里。方法开发和方法验证的目标是最适合 通过FDA和弗恩的指导，佛罗里达州和全国的食品检测需求。 食品安全实验室部门愿意并有能力参与这一机会和 全力致力于提高我国食品检测的分析能力和能力 实验室，通过食品防御和食品安全保护国家的粮食供应 监测活动，从而加强保护粮食供应的努力，努力实现 保障公众健康。

Discipline D: Special Projects Analytical Track 3: Method Development and Method Validation Project Summary/Abstract: The Florida Department of Agriculture and Consumer Services (FDACS), Division of Food Safety Laboratories achieved accreditation in 2007 and maintains A2LA accreditation to ISO/IEC 17025:2017 for the specific tests listed in A2LA certificates 2534.01, 2534.02 and 2534.03 (available at www.A2LA.org). The Division has the responsibility for carrying out its duties or support functions as directed by the following Florida Statutes Chapters: 500, 502, 583, 586, and 601. The Division of Food Safety is responsible for assuring the public of a safe, wholesome and properly represented food supply. This is accomplished through the permitting and inspection of food establishments; inspection and evaluation of food and dairy products; and the performance of biological and chemical laboratory testing on a variety of food and dairy products. The Division of Food Safety Laboratories are applying for the Funding Opportunity titled: Laboratory Flexible Funding Model (LFFM), announcement number PAR-20-105, with anticipation of funding from 2020 through 2025 for enhancing the capacity and capabilities of method development and method validation in the areas of Microbiology and Chemistry. The Laboratories are currently funded under the Florida Microbiology and Chemistry FDA FERN Program, 2015 – 2020 (5U18FD005506-05). The DFS-Bio Microbiology program has been an active funded laboratory since the inception on FERN microbiology program in 2004 and has participated in microbiology method development and method validation for FERN and the FDA. DFS-Bio has undertaken large-scale method development projects including the development of a Shiga-toxin producing E. coli (STEC) real-time PCR testing method for both O157 and non- O157 “Big Six” E. coli, and the development of a new method to test for Cyclospora cayetanensis in fresh herbs. Method development and method validation has been targeted to best suit the food testing needs of both the State of Florida and the nation through FDA and FERN direction. The Division of Food Safety Laboratories are willing and able to participate in this opportunity and are fully committed to increase the analytical capability and capacity of our nation’s food testing laboratories so as to protect the nation’s food supply through food defense and food safety surveillance activities, and thereby enhance efforts to protect the food supply and work toward ensuring the public’s health.