Metabolomics Core

代谢组学核心

• 批准号: 10171105
• 负责人: John M Koomen
• 金额: $ 19.29万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-25 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10171105
• 关键词: Address; Archives; Biological; Biological Assay; Cancer Biology; Cancer Center; Cancer Patient; Cell Communication; Cell Line; Collaborations; Complex; Computer software; Conceptions; Core Grant; Cysteine Metabolism Pathway; Data; Data Analyses; Data Science; Data Science Core; Data Scientist; Data Set; Development; Enzyme Inhibition; Enzymes; Equipment; Experimental Designs; Faculty; Family member; Gas Chromatography; Gas-Liquid Chromatography; Genetically Engineered Mouse; Genomics; Glycolysis; Goals; Grant; Guidelines; Human; Immune system; Individual; Intervention; Knowledge; Location; Lung Neoplasms; Malignant neoplasm of lung; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Metabolism; Methods; Molecular; Monitor; Myeloid-derived suppressor cells; Pathology; Pathway interactions; Phenotype; Play; Post Translational Modification Analysis; Pramlintide; Pre-Clinical Model; Preparation; Program Research Project Grants; Protein Analysis; Proteins; Proteomics; Publications; Publishing; Quality Control; Research; Research Personnel; Research Support; Role; Sampling; Services; Stable Isotope Labeling; Sulfhydryl Compounds; TP53 gene; Techniques; Testing; Tumor Biology; Tumor Escape; Tumor-infiltrating immune cells; Work; Xenograft procedure; activity-based protein profiling; assay development; base; data acquisition; data repository; design; experience; experimental study; improved outcome; interest; lipid metabolism; liquid chromatography mass spectrometry; lung small cell carcinoma; member; metabolomics; method development; molecular scale; multiple datasets; novel; potential biomarker; preservation; public repository; quality assurance; response; sample collection; success; synergism; tool; tumor; tumor immunology; tumor metabolism

CORE 3 PROJECT SUMMARY METABOLISM CORE The Metabolism Core (Core #3) supports the metabolomics and proteomics experiments designed by program project grant (PPG) investigators as integral parts of their proposed research. These large-scale molecular data analysis strategies are used by all four projects in this PPG, which provides synergistic linkage points, facilitated by Core #3, across projects for cross identification of metabolites and proteins that play important roles in lung cancer biology and immunology. Core #3 members have extensive experience in cancer metabolism, metabolomics, proteomics, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, collaboration with investigators, collaboration with data scientists (including members of Core #4), and customer service. These efforts will contribute to projects from inception to completion (i.e. from hypothesis to publication). Core #3 will support optimization of experimental design for mass spectrometry- based omics (Aim 1), which includes implementation of previously published strategies and development of novel methods for sample preparation, separations including gas and liquid chromatography, and mass spectrometry to support the experiments required by PPG investigators. Datasets will be acquired with untargeted metabolomics, discovery proteomics, analysis of post-translational modifications, and targeted assay development for metabolites and proteins utilizing state-of-the-art separations and mass spectrometry equipment at Moffitt Cancer Center (Aim 2). Core #3 members will contribute to quality assurance/quality control for data acquired for each project and collaborate with PPG investigators and other PPG Cores to produce high quality datasets and develop data analysis strategies to maximize availability and usage of omics data across PPG Projects (Aim 3). In summary, Core #3 will make a significant contribution to the proposed research by elucidating molecular mechanisms underlying lung tumor biology and tumor-immune cell interactions, which may ultimately reveal potential biomarkers and novel treatment targets to be further investigated in functional studies proposed by the PPG Projects.

核心3项目总结 代谢核心 代谢核心（核心#3）支持代谢组学和蛋白质组学实验， 项目资助（PPG）研究人员作为其拟议研究的组成部分。这些大规模的分子 本项目规划中的所有四个项目都采用了数据分析战略，提供了协同联系点， 在核心#3的推动下，跨项目交叉鉴定代谢物和蛋白质， 在肺癌生物学和免疫学中的作用。核心#3成员在癌症方面有丰富的经验 代谢、代谢组学、蛋白质组学、液相色谱-质谱、气相色谱-质谱 光谱分析，与调查人员合作，与数据科学家（包括核心小组成员）合作 #4、客户服务。这些努力将有助于项目从开始到完成（即从 发表论文）。核心3将支持质谱实验设计的优化- 基于组学（目标1），其中包括实施先前公布的战略和开发 用于样品制备、分离（包括气相色谱和液相色谱）和质谱的新方法 光谱分析，以支持PPG研究人员所需的实验。数据集将通过 非靶向代谢组学、发现蛋白质组学、翻译后修饰分析和靶向 利用最先进的分离和质谱技术开发代谢物和蛋白质的分析方法 Moffitt癌症中心（Aim 2）。核心#3成员将为质量保证/质量做出贡献 控制每个项目采集的数据，并与PPG调查员和其他PPG核心合作， 生成高质量的数据集，并制定数据分析策略，以最大限度地提高组学的可用性和使用率 PPG项目的数据（目标3）。总之，核心#3将对拟议的 通过阐明肺肿瘤生物学和肿瘤免疫细胞的分子机制进行研究 相互作用，这可能最终揭示潜在的生物标志物和新的治疗靶点，以进一步 在PPG项目提出的功能研究中进行了调查。