Metabolomics Core

代谢组学核心

基本信息

批准号：
10438719
负责人：
John M Koomen
金额：
$ 18.77万
依托单位：
H. LEE MOFFITT CANCER CTR & RES INST
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-25 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10438719
关键词：
Address Archives Biological Biological Assay Cancer Biology Cancer Center Cancer Patient Cell Communication Cell Line Collaborations Complex Computer software Conceptions Core Grant Cysteine Metabolism Pathway Data Data Analyses Data Science Data Science Core Data Scientist Data Set Development Enzyme Inhibition Enzymes Equipment Experimental Designs Faculty Family member Gas Chromatography Gas-Liquid Chromatography Genetically Engineered Mouse Genomics Glycolysis Goals Grant Guidelines Human Immune system Individual Intervention Knowledge Location Lung Neoplasms Malignant neoplasm of lung Mass Spectrum Analysis Metabolic Metabolic Pathway Metabolism Methods Molecular Monitor Myeloid-derived suppressor cells Pathology Pathway interactions Phenotype Play Post Translational Modification Analysis Pramlintide Pre-Clinical Model Preparation Program Research Project Grants Protein Analysis Proteins Proteomics Publications Publishing Quality Control Research Research Personnel Research Support Role Sampling Services Stable Isotope Labeling Sulfhydryl Compounds TP53 gene Techniques Testing Tumor Biology Tumor Escape Tumor-infiltrating immune cells Work Xenograft procedure activity-based protein profiling assay development base data acquisition data repository design experience experimental study improved outcome interest lipid metabolism liquid chromatography mass spectrometry lung small cell carcinoma member metabolomics method development molecular scale multiple datasets novel potential biomarker preservation public repository quality assurance response sample collection success synergism tool tumor tumor immunology tumor metabolism

项目摘要

CORE 3 PROJECT SUMMARY METABOLISM CORE The Metabolism Core (Core #3) supports the metabolomics and proteomics experiments designed by program project grant (PPG) investigators as integral parts of their proposed research. These large-scale molecular data analysis strategies are used by all four projects in this PPG, which provides synergistic linkage points, facilitated by Core #3, across projects for cross identification of metabolites and proteins that play important roles in lung cancer biology and immunology. Core #3 members have extensive experience in cancer metabolism, metabolomics, proteomics, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, collaboration with investigators, collaboration with data scientists (including members of Core #4), and customer service. These efforts will contribute to projects from inception to completion (i.e. from hypothesis to publication). Core #3 will support optimization of experimental design for mass spectrometry- based omics (Aim 1), which includes implementation of previously published strategies and development of novel methods for sample preparation, separations including gas and liquid chromatography, and mass spectrometry to support the experiments required by PPG investigators. Datasets will be acquired with untargeted metabolomics, discovery proteomics, analysis of post-translational modifications, and targeted assay development for metabolites and proteins utilizing state-of-the-art separations and mass spectrometry equipment at Moffitt Cancer Center (Aim 2). Core #3 members will contribute to quality assurance/quality control for data acquired for each project and collaborate with PPG investigators and other PPG Cores to produce high quality datasets and develop data analysis strategies to maximize availability and usage of omics data across PPG Projects (Aim 3). In summary, Core #3 will make a significant contribution to the proposed research by elucidating molecular mechanisms underlying lung tumor biology and tumor-immune cell interactions, which may ultimately reveal potential biomarkers and novel treatment targets to be further investigated in functional studies proposed by the PPG Projects.

核心3项目摘要代谢核心代谢核心（Core＃3）支持由程序设计的代谢组学和蛋白质组学实验项目赠款（PPG）研究人员是其拟议研究的组成部分。这些大型分子该PPG中的所有四个项目都使用数据分析策略，该项目提供协同的链接点，由Core＃3促进的跨越跨项目，用于交叉识别发挥重要作用的代谢物和蛋白质在肺癌生物学和免疫学中的作用。核心＃3成员在癌症方面有丰富的经验代谢，代谢组学，蛋白质组学，液相色谱质量光谱法，气相色谱 - 质量 - 光谱法，与研究人员合作，与数据科学家合作（包括核心成员＃4）和客户服务。这些努力将有助于从成立到完成的项目（即出版的假设）。 Core＃3将支持质谱法实验设计的优化 - 基于OMICS（AIM 1），其中包括实施先前发表的策略和制定样品制备的新方法，包括气体和液相色谱的分离以及质量光谱法支持PPG研究者要求的实验。数据集将与未定位的代谢组学，发现蛋白质组学，翻译后修饰的分析和针对性的使用最先进的分离和质谱法的代谢物和蛋白质的测定开发莫菲特癌症中心的设备（AIM 2）。核心＃3成员将有助于质量保证/质量为每个项目获得的数据控制，并与PPG调查人员和其他PPG核心合作生产高质量的数据集并制定数据分析策略，以最大程度地利用OMICS的可用性和使用跨PPG项目的数据（AIM 3）。总而言之，核心＃3将为拟议的通过阐明肺肿瘤生物学和肿瘤免疫细胞的分子机制的研究相互作用最终可能揭示潜在的生物标志物和新型治疗靶标需要进一步 PPG项目提出的功能研究中进行了研究。