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Metabolomics Core

代谢组学核心

基本信息

批准号：
10676752
负责人：
John M Koomen
金额：
$ 18.9万
依托单位：
H. LEE MOFFITT CANCER CTR & RES INST
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-25 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10676752
关键词：
Address Archives Biological Biological Assay Cancer Biology Cancer Center Cancer Patient Cell Communication Cell Line Collaborations Complex Computer software Conceptions Core Grant Cysteine Metabolism Pathway Data Data Analyses Data Science Data Science Core Data Scientist Data Set Dedications Development Enzyme Inhibition Enzymes Equipment Experimental Designs Faculty Family member Gas Chromatography Genetically Engineered Mouse Genomics Glycolysis Goals Grant Guidelines Human Immune system Individual Intervention Knowledge Location Lung Neoplasms Malignant neoplasm of lung Maps Mass Chromatography Mass Fragmentography Mass Spectrum Analysis Metabolic Metabolic Pathway Metabolism Methods Molecular Monitor Myeloid-derived suppressor cells Pathology Pathway interactions Phenotype Play Post Translational Modification Analysis Pramlintide Pre-Clinical Model Preparation Program Research Project Grants Protein Analysis Proteins Proteomics Publications Publishing Quality Control Research Research Personnel Research Support Role Sampling Services Stable Isotope Labeling Sulfhydryl Compounds TP53 gene Techniques Testing Tumor Biology Tumor Escape Tumor-infiltrating immune cells Work Xenograft procedure activity-based protein profiling assay development data acquisition data integration data repository design experience experimental study improved outcome interest lipid metabolism liquid chromatography mass spectrometry member metabolomics method development molecular scale multiple datasets novel potential biomarker preservation public repository quality assurance response sample collection small cell lung carcinoma success synergism tool tumor tumor immunology tumor metabolism

项目摘要

CORE 3 PROJECT SUMMARY METABOLISM CORE The Metabolism Core (Core #3) supports the metabolomics and proteomics experiments designed by program project grant (PPG) investigators as integral parts of their proposed research. These large-scale molecular data analysis strategies are used by all four projects in this PPG, which provides synergistic linkage points, facilitated by Core #3, across projects for cross identification of metabolites and proteins that play important roles in lung cancer biology and immunology. Core #3 members have extensive experience in cancer metabolism, metabolomics, proteomics, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, collaboration with investigators, collaboration with data scientists (including members of Core #4), and customer service. These efforts will contribute to projects from inception to completion (i.e. from hypothesis to publication). Core #3 will support optimization of experimental design for mass spectrometry- based omics (Aim 1), which includes implementation of previously published strategies and development of novel methods for sample preparation, separations including gas and liquid chromatography, and mass spectrometry to support the experiments required by PPG investigators. Datasets will be acquired with untargeted metabolomics, discovery proteomics, analysis of post-translational modifications, and targeted assay development for metabolites and proteins utilizing state-of-the-art separations and mass spectrometry equipment at Moffitt Cancer Center (Aim 2). Core #3 members will contribute to quality assurance/quality control for data acquired for each project and collaborate with PPG investigators and other PPG Cores to produce high quality datasets and develop data analysis strategies to maximize availability and usage of omics data across PPG Projects (Aim 3). In summary, Core #3 will make a significant contribution to the proposed research by elucidating molecular mechanisms underlying lung tumor biology and tumor-immune cell interactions, which may ultimately reveal potential biomarkers and novel treatment targets to be further investigated in functional studies proposed by the PPG Projects.

Core 3项目总结