Proteomics & Metabolomics Core

蛋白质组学

• 批准号: 10558786
• 负责人: John M Koomen
• 金额: --
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-18 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10558786
• 关键词: Address; Advisory Committees; Advocate; Area; Bioinformatics Shared Resource; Biological Assay; Biological Markers; Biometry; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cellular Metabolic Process; Chemicals; Chromatography; Clinical Trials; Collaborations; Communities; Complement; Conceptions; Data; Data Analyses; Development; Doctor of Philosophy; Drug resistance; Education; Ensure; Equipment; Experimental Designs; Faculty; Feasibility Studies; Funding; Funding Agency; Future; Goals; Grant; Group Meetings; High School Student; Immune; Internships; Investigation; Investments; Isotopes; Journals; Knowledge; Laboratories; Liquid Chromatography; Literature; Malignant Neoplasms; Manuscripts; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Measures; Medical Students; Methods; Molecular; Organism; Organizational Objectives; Patients; Peer Review; Peptides; Phenotype; Postdoctoral Fellow; Preparation; Procedures; Proteins; Proteomics; Protocols documentation; Publications; Reproducibility; Research; Resource Sharing; Resources; Sampling; Science; Scientist; Services; Software Tools; Specialist; Strategic Planning; Techniques; Technology; Text; Tissues; Training; Vendor; Work; anticancer research; assay development; cancer proteomics; college; data analysis pipeline; data integration; data sharing; data submission; experience; experimental study; improved; innovation; instrument; instrumentation; lipidomics; member; metabolomics; new technology; novel; novel strategies; programs; proteogenomics; response; student training; targeted treatment; theories; tool development; tumor metabolism; undergraduate student

PROJECT SUMMARY PROTEOMICS & METABOLOMICS CORE The goal of the Moffitt Proteomics & Metabolomics Core (P&MC) is to provide state-of-the-art equipment, cutting- edge services, comprehensive education, and hands-on training in proteomics and metabolomics techniques. P&MC was launched in 2003 to provide proteomic profiling. In 2016, metabolomics services were added to support cancer metabolism, a key priority area of Moffitt’s Research Strategic Plan. Highly trained and experienced core staff provide domain knowledge, customer service, and extensive support from project conception to publication, leveraging cutting edge liquid chromatography- and gas chromatography-mass spectrometry platforms complemented by diverse sample preparation techniques and data analysis strategies. P&MC provides Member access to highly trained expert staff, leading edge proteomics and metabolomics instrumentation, software tools, and the capability to perform appropriate experiments to address their hypotheses. P&MC activities towards its goals are organized into three Specific Aims: Aim 1: To provide proteomics and metabolomics expertise and resources to support Moffitt Member research. Aim 2: To provide services, support publications and grants, and adapt to Member needs in cancer proteomics and metabolomics, by developing novel capabilities that support the diverse research of Moffit CCSG Programs. Aim 3: To educate and train Moffitt faculty, trainees, laboratory staff and the community on the theory and practice of proteomics and metabolomics in cancer research. P&MC significantly contributes to Member science at Moffitt. For example, P&MC worked with other Shared Resources (e.g., MGC, BBSR) to integrate data supporting key proteogenomics and proteometabolomics projects. Other key areas include: 1) development of tools to dissect the response to targeted therapy and assess drug resistance; 2) increased capability to study cancer metabolism at the cellular and organism level, and 3) sensitive and specific quantification of structurally similar proteins. This contribution is evidenced by P&MC supporting 79 Members over the last five years, a 30% increase in Member usage since 2016. Members used P&MC services for highly multiplexed measurements of proteins and metabolites to assess cancer phenotypes, complementing traditional cancer biology approaches, leading to 50 publications (15 high impact). In FY20, P&MC supported 32 Members in four Programs (CBE 31%, CE 6%, MM 38%, IO 25%) with 96% of those users holding peer reviewed funding. This represented 95% of all core usage. Future priorities of P&MC are to maintain exceptional service, improve quantitative proteomics, enhance metabolomic services, expand support of diverse biomarker initiatives, and further build on pan-omic data analysis pipelines to enhance rigor and reproducibility, and to maximize the knowledge gained from proteomic and metabolomic investigations.

项目摘要 蛋白质组学和代谢组学核心 Moffitt蛋白质组学和代谢组学核心（P＆MC）的目标是提供最先进的设备，替代设备 - 边缘服务，综合教育以及蛋白质组学和代谢组学技术方面的动手培训。 P＆MC于2003年推出，以提供蛋白质组学分析。 2016年，代谢组学服务被添加到 支持癌症代谢，这是莫菲特研究战略计划的关键优先领域。训练有素和 经验丰富的核心人员提供域知识，客户服务和项目的广泛支持 出版的概念，利用尖端液相色谱和气相色谱质量 由潜水员样品制备技术和数据分析策略完成的光谱平台。 P＆MC为成员提供了训练有素的专家员工，前沿蛋白质组学和代谢组学的访问权限 仪器，软件工具以及执行适当实验的能力以解决其 假设。 P＆C MC的目标将其目标组织为三个具体目标： 目标1：提供蛋白质组学和代谢组学专业知识和资源来支持Moffitt成员研究。 目标2：提供服务，支持出版物和赠款，并适应癌症蛋白质组学的成员需求 和代谢组学，通过开发支持Moffit CCSG计划的潜水员研究的新型能力。 目标3：教育和培训莫菲特的教职员工，实验室工作人员和社区理论以及 蛋白质组学和代谢组学实践中的癌症研究。 P＆M MC在莫菲特的会员科学上做出了重大贡献。例如，P＆MC与其他共享 资源（例如MGC，BBSR）集成支持关键蛋白质组学和蛋白质代谢组学的数据 项目。其他关键领域包括：1）开发用于剖析目标治疗和评估反应的工具 耐药性； 2）提高了在细胞和生物水平研究癌症代谢的能力，3） 敏感和特异性的结构相似蛋白质。 P＆MC证明了这一贡献 在过去五年中支持79名成员，自2016年以来的会员使用率增加了30％。 P＆C MC服务，用于高度多路复用的蛋白质和代谢物，以评估癌症表型， 补充传统的癌症生物学方法，导致50种出版物（15个高影响力）。在20财年， P＆MC在四个计划中支持32名成员（CBE 31％，CE 6％，MM 38％，IO 25％），其中96％ 持有同行审查的资金。这占所有核心用法的95％。 P＆C的未来优先事项是维护 卓越的服务，改善定量蛋白质组学，增强代谢组学，扩大潜水员的支持 生物标志物计划，并进一步建立在泛 - 运动数据分析管道上，以增强严格性和可重复性， 并最大化蛋白质组学和代谢组学研究所获得的知识。