Proteomics & Metabolomics Core

蛋白质组学

• 批准号: 10333180
• 负责人: John M Koomen
• 金额: $ 16.22万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-18 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10333180
• 关键词: Address; Advisory Committees; Advocate; Area; Bioinformatics Shared Resource; Biological Assay; Biological Markers; Biometry; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cellular Metabolic Process; Chemicals; Chromatography; Clinical Trials; Communities; Complement; Conceptions; Data; Data Analyses; Development; Doctor of Philosophy; Drug resistance; Education; Ensure; Equipment; Experimental Designs; Faculty; Feasibility Studies; Funding; Funding Agency; Future; Goals; Grant; Group Meetings; High School Student; Immune; Internships; Investigation; Investments; Isotopes; Journals; Knowledge; Laboratories; Liquid Chromatography; Literature; Malignant Neoplasms; Manuscripts; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Measures; Medical Students; Methods; Molecular; Organism; Patients; Peer Review; Peptides; Phenotype; Postdoctoral Fellow; Preparation; Procedures; Proteins; Proteomics; Protocols documentation; Publications; Reproducibility; Research; Resource Sharing; Resources; Sampling; Science; Scientist; Services; Software Tools; Specialist; Strategic Planning; Techniques; Technology; Text; Tissues; Training; Vendor; Work; anticancer research; assay development; cancer proteomics; college; data analysis pipeline; data sharing; data submission; experience; experimental study; improved; innovation; instrument; instrumentation; lipidomics; member; metabolomics; new technology; novel; novel strategies; programs; protein metabolite; proteogenomics; response; student training; targeted treatment; theories; tool development; tumor metabolism; undergraduate student

PROJECT SUMMARY PROTEOMICS & METABOLOMICS CORE The goal of the Moffitt Proteomics & Metabolomics Core (P&MC) is to provide state-of-the-art equipment, cutting- edge services, comprehensive education, and hands-on training in proteomics and metabolomics techniques. P&MC was launched in 2003 to provide proteomic profiling. In 2016, metabolomics services were added to support cancer metabolism, a key priority area of Moffitt’s Research Strategic Plan. Highly trained and experienced core staff provide domain knowledge, customer service, and extensive support from project conception to publication, leveraging cutting edge liquid chromatography- and gas chromatography-mass spectrometry platforms complemented by diverse sample preparation techniques and data analysis strategies. P&MC provides Member access to highly trained expert staff, leading edge proteomics and metabolomics instrumentation, software tools, and the capability to perform appropriate experiments to address their hypotheses. P&MC activities towards its goals are organized into three Specific Aims: Aim 1: To provide proteomics and metabolomics expertise and resources to support Moffitt Member research. Aim 2: To provide services, support publications and grants, and adapt to Member needs in cancer proteomics and metabolomics, by developing novel capabilities that support the diverse research of Moffit CCSG Programs. Aim 3: To educate and train Moffitt faculty, trainees, laboratory staff and the community on the theory and practice of proteomics and metabolomics in cancer research. P&MC significantly contributes to Member science at Moffitt. For example, P&MC worked with other Shared Resources (e.g., MGC, BBSR) to integrate data supporting key proteogenomics and proteometabolomics projects. Other key areas include: 1) development of tools to dissect the response to targeted therapy and assess drug resistance; 2) increased capability to study cancer metabolism at the cellular and organism level, and 3) sensitive and specific quantification of structurally similar proteins. This contribution is evidenced by P&MC supporting 79 Members over the last five years, a 30% increase in Member usage since 2016. Members used P&MC services for highly multiplexed measurements of proteins and metabolites to assess cancer phenotypes, complementing traditional cancer biology approaches, leading to 50 publications (15 high impact). In FY20, P&MC supported 32 Members in four Programs (CBE 31%, CE 6%, MM 38%, IO 25%) with 96% of those users holding peer reviewed funding. This represented 95% of all core usage. Future priorities of P&MC are to maintain exceptional service, improve quantitative proteomics, enhance metabolomic services, expand support of diverse biomarker initiatives, and further build on pan-omic data analysis pipelines to enhance rigor and reproducibility, and to maximize the knowledge gained from proteomic and metabolomic investigations.

项目摘要 蛋白质组学和代谢组学核心 莫菲特蛋白质组学和代谢组学核心（P&MC）的目标是提供最先进的设备，切割- 边缘服务，全面的教育，以及蛋白质组学和代谢组学技术的实践培训。 P&MC成立于2003年，提供蛋白质组学分析。2016年，代谢组学服务被添加到 支持癌症代谢，这是莫菲特研究战略计划的一个关键优先领域。高度训练和 经验丰富的核心员工提供领域知识，客户服务和项目的广泛支持 从概念到出版，利用最先进的液相色谱和气相色谱质谱 光谱分析平台辅以各种样品制备技术和数据分析策略。 P&MC为会员提供训练有素的专家工作人员，领先的蛋白质组学和代谢组学 仪器，软件工具，以及执行适当实验以解决其 假设P&MC为实现其目标而开展的活动分为三个具体目标： 目标1：提供蛋白质组学和代谢组学的专业知识和资源，以支持莫菲特成员的研究。 目标2：提供服务，支持出版物和赠款，并适应会员在癌症蛋白质组学方面的需求 和代谢组学，通过开发新的能力，支持Moffit CCSG计划的多样化研究。 目标3：教育和培训莫菲特教师，学员，实验室工作人员和社区的理论和 蛋白质组学和代谢组学在癌症研究中的应用。 P&MC为Moffitt的会员科学做出了重大贡献。例如，P&MC与其他共享 资源（例如，MGC、BBSR）整合支持关键蛋白基因组学和蛋白代谢组学的数据 项目其他关键领域包括：1）开发工具来分析对靶向治疗的反应， 耐药性; 2）在细胞和生物体水平研究癌症代谢的能力增加，以及3） 结构相似蛋白质的灵敏和特异性定量。P&MC的这一贡献得到了证明。 在过去的五年里支持了79个会员，自2016年以来会员使用量增加了30%。成员使用 P&MC服务，用于蛋白质和代谢物的高度多重测量，以评估癌症表型， 补充传统的癌症生物学方法，导致50篇出版物（15篇高影响力）。在FY 20， P&MC在四个项目中支持了32名成员（CBE 31%，CE 6%，MM 38%，IO 25%），其中96%的用户 进行同行评审的资金。这占所有核心使用量的95%。P&MC未来的优先事项是保持 卓越的服务，改善定量蛋白质组学，增强代谢组学服务，扩大对各种 生物标志物倡议，并进一步建立泛组学数据分析管道，以提高严谨性和可重复性， 并最大化从蛋白质组学和代谢组学研究中获得的知识。