Intergrated Biorepository of H3Africa Uganda

总结/摘要 背景：高质量的生物和环境标本的可利用性以及注释的研究 目的要求制定标准化的方法，用于收集、处理、长期储存， 检索和分发标本，使其能够在未来用于研究。 因此，NIH（2013年）与Makerere大学一起建立了 乌干达的H3非洲（IBRH 3AU）是一个最先进的样本采集、处理、储存和检索设施 并为H3非洲联盟和其他研究人员提供基因组研究。的总目标 该提案使我们能够将现有活动与最近授予的时间表保持一致， 或更新H3非洲项目，同时扩大我们的范围，包括环境样本， 最终的可持续性。这一目标有四个具体目标，即（一）继续提供生物储存库， 为H3非洲项目提供服务（样本采集和/或接收、处理、储存、检索和运输）， 其他研究人员，（ii）扩大我们的样本基础以外的核酸，包括其他类型和环境 样本，㈢扩大和巩固可持续性计划，㈣放置一个可靠的生物标本 H3 Africa样本命运的继任计划。 方法：（a）将按照国际生物资源研究所的最佳做法提供质量控制和有保证的生物贮藏服务 （B）除了现有的技术和标准外， 并实施了基因组分析方案（血斑DNA、RNA等），微生物组分析 （阴道、鼻腔、粪便拭子等），HIV或其他传染病研究（PBMC处理、病毒载量、病毒 测序、CD 4/CD 8计数、免疫测定等），生物标志物分析，更多方案，标准 将制定和实施包括适当储存措施的 环境暴露（烟草烟雾、有毒金属、类金属和矿物质营养素、 杀虫剂、霉菌毒素、多环芳烃代谢物、挥发性有机化合物等）（c）第（1）款 通过继续执行我们的业务计划，以确保可持续性，以及（d）通过协商 和共识，为资助者、H3非洲财团和IBRH 3AU制定一个可行的继任计划。 预期收益： 为生物储存政策准则作出贡献 促进基因组/遗传研究 通过在线目录可访问的注释良好的质量控制生物标本设施 生物储藏科学和管理培训。 探索存储样品的附加价值

Summary/Abstract Background: The availability of high quality well annotated biological and environmental specimens for research purposes requires the development of standardized methods for collection, processing, long-term storage, retrieval and distribution of specimens that will enable their future use for research. For this reason the NIH (in 2013) together with Makerere University established the Integrated Biorepository of H3Africa in Uganda (IBRH3AU) is a state of the art facility for sample collection, processing, storage, retrieval and distribution for genomic research for the H3Africa Consortium and other researchers. The overall goal of this proposal is to enable us align our existing activities with the timelines of the recently awarded and or renewed H3Africa projects, while expanding our scope, including environmental samples, and ultimate sustainability. Four specific aims are contingent to this goal, viz (i) continue to offer biorepository services (sample collection and or receipt, processing, storage, retrieval and shipment) to H3Africa projects and other researchers, (ii) expand our sample base beyond Nucleic acids to include other-types and environmental samples, (iii) Scale up and consolidate the sustainability plan, and (iv) Put-in-place an amenable biospecimen succession plan for the destiny of H3Africa samples. Approach: (a) Quality controlled and assured biorepository services will be offered as per ISBER best practices and standards, in line with H3Africa Data and Biospecimen Accession (DBAC) policies, (b) In addition to existing and implemented protocols for genomic analyses (DNA from blood spots, RNA, etc.), microbiome analyses (vaginal, nasal, fecal swabs, etc.), HIV or other infectious disease research (PBMC processing, viral loads, viral sequencing, CD4/CD8 counts, immunological assays, etc.), Biomarker analyses, More Protocols, Standards and Practices will be developed and implemented including for appropriate storage for measures of environmental exposures (tobacco smoke, toxic metals, metalloids, and mineral nutrients, metabolites of pesticides, toxins from mold, polycyclic aromatic hydrocarbon metabolites, volatile organic compounds, etc.) (c) Through continued implementation of our business plan to ensure sustainability, and (d) through consultations and consensus, devise an amenable succession plan for the funders, H3Africa consortium, and IBRH3AU. Expected Benefits: Contribute to biorepository policy guidelines Stimulate genomic/genetic research A facility of well annotated quality controlled biospecimen accessible through an online catalog Biorepository science and management trainings. Explore value addition to stored samples