Intergrated Biorepository of H3Africa Uganda

H3Africa 乌干达综合生物样本库

• 批准号: 9797631
• 负责人: Moses Lutaakome Joloba
• 金额: $ 90万
• 依托单位: MAKERERE UNIVERSITY COLLEGE OF HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-20 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9797631
• 关键词: Achievement; Address; Africa; African; Aromatic Polycyclic Hydrocarbons; Asthma; Award; Bioinformatics; Biological; Biological Markers; Blood; Budgets; Businesses; CD8B1 gene; Catalogs; Central Africa; Collection; Communities; Complex; Consensus; Consultations; DNA; DNA Adducts; Data; Development; Diabetes Mellitus; Disease; Eastern Africa; Ensure; Environment; Environmental Exposure; Event; Exhibits; Face; Funding; Future; Genes; Genetic; Genetic Diseases; Genetic Research; Genetic Variation; Genomics; Goals; Guidelines; HIV; Health; Heredity; Human; Human Resources; Immunology procedure; Infectious Diseases Research; Infrastructure; Label; Laboratories; Life Style; Light; Malnutrition; Mass Spectrum Analysis; Measures; Methods; Minerals; Molds; Molecular Biology; Molecular Immunology; Nose; Nucleic Acids; Nutrient; Peripheral Blood Mononuclear Cell; Pesticides; Plasma; Policies; Population; Procedures; Process; Protocols documentation; Quality Control; RNA; Research; Research Personnel; Research Project Grants; Research Support; Research Training; Retrieval; Sampling; Science; Service provision; Services; Shipping; Ships; Site; Southern Africa; Specimen; Specimen Handling; Spottings; Standardization; Structure; Students; Swab; Technology; TimeLine; Tobacco smoke; Toxin; Training; Training Activity; Training Programs; Uganda; United States National Institutes of Health; Universities; Urine; Vagina; Viral; Viral Load result; Voting; Western Africa; base; biobank; genomic data; graduate student; health management; meetings; microbiome analysis; pesticide exposure; programs; reconstitution; repository; sample collection; scale up; skills; toxic metal; volatile organic compound

Summary/Abstract Background: The availability of high quality well annotated biological and environmental specimens for research purposes requires the development of standardized methods for collection, processing, long-term storage, retrieval and distribution of specimens that will enable their future use for research. For this reason the NIH (in 2013) together with Makerere University established the Integrated Biorepository of H3Africa in Uganda (IBRH3AU) is a state of the art facility for sample collection, processing, storage, retrieval and distribution for genomic research for the H3Africa Consortium and other researchers. The overall goal of this proposal is to enable us align our existing activities with the timelines of the recently awarded and or renewed H3Africa projects, while expanding our scope, including environmental samples, and ultimate sustainability. Four specific aims are contingent to this goal, viz (i) continue to offer biorepository services (sample collection and or receipt, processing, storage, retrieval and shipment) to H3Africa projects and other researchers, (ii) expand our sample base beyond Nucleic acids to include other-types and environmental samples, (iii) Scale up and consolidate the sustainability plan, and (iv) Put-in-place an amenable biospecimen succession plan for the destiny of H3Africa samples. Approach: (a) Quality controlled and assured biorepository services will be offered as per ISBER best practices and standards, in line with H3Africa Data and Biospecimen Accession (DBAC) policies, (b) In addition to existing and implemented protocols for genomic analyses (DNA from blood spots, RNA, etc.), microbiome analyses (vaginal, nasal, fecal swabs, etc.), HIV or other infectious disease research (PBMC processing, viral loads, viral sequencing, CD4/CD8 counts, immunological assays, etc.), Biomarker analyses, More Protocols, Standards and Practices will be developed and implemented including for appropriate storage for measures of environmental exposures (tobacco smoke, toxic metals, metalloids, and mineral nutrients, metabolites of pesticides, toxins from mold, polycyclic aromatic hydrocarbon metabolites, volatile organic compounds, etc.) (c) Through continued implementation of our business plan to ensure sustainability, and (d) through consultations and consensus, devise an amenable succession plan for the funders, H3Africa consortium, and IBRH3AU. Expected Benefits: Contribute to biorepository policy guidelines Stimulate genomic/genetic research A facility of well annotated quality controlled biospecimen accessible through an online catalog Biorepository science and management trainings. Explore value addition to stored samples

摘要/摘要 背景：用于研究的高质量、注解良好的生物和环境标本的可用性 目的要求开发标准化的收集、处理、长期储存、 检索和分发标本，以便将来将其用于研究。 为此，美国国立卫生研究院(2013年)与Makerere大学共同建立了 H3非洲在乌干达(IBRH3AU)是一家最先进的样本收集、处理、存储和检索设施 并为H3非洲联盟和其他研究人员提供基因组研究。的总目标是 这项提议是为了使我们能够使我们现有的活动与最近授予的和 或更新H3非洲项目，同时扩大我们的范围，包括环境样本，以及 终极可持续性。四个具体目标取决于这一目标，即(I)继续提供生物库 向H3非洲项目提供服务(样品收集和/或接收、加工、储存、取回和运输)和 其他研究人员，(Ii)将我们的样本库扩大到核酸以外，包括其他类型和环境 (3)扩大和巩固可持续发展计划；(4)建立一个可服从的生物生态系统 H3非洲样本命运的接班计划。 办法：(A)将按照ISBER最佳做法提供有质量控制和有保证的生物库服务 和标准，符合H3非洲数据和生物质学家加入(DBAC)政策，(B)除了现有的 并实施了基因组分析(来自血点的DNA、RNA等)、微生物组分析 (阴道、鼻腔、粪便拭子等)、艾滋病毒或其他传染病研究(PBMC处理、病毒载量、病毒 测序、CD4/CD8计数、免疫学分析等)、生物标志物分析、更多方案、标准 并将制定和实施各种做法，包括适当储存下列措施 环境暴露(烟草烟雾、有毒金属、类金属和矿物质营养素、 杀虫剂、霉菌毒素、多环芳烃代谢物、挥发性有机化合物等)(C) 通过继续执行我们的业务计划以确保可持续性，以及(D)通过协商 并达成共识，为资助者、H3Africa财团和IBRH3AU制定一项顺从的继任计划。 预期收益： 为生物信息库政策指南做出贡献 促进基因组/遗传学研究 可通过在线目录访问的具有良好注释的质量受控生物显微镜的设施 生物库科学和管理培训。 探索存储样本的附加值