Intergrated Biorepository of H3Africa Uganda

摘要/摘要 背景：高质量、注解良好的生物和环境的可用性 用于研究目的的标本需要开发标准化方法 收集、处理、长期储存、检索和分发将 使它们能够在未来用于研究。 出于这个原因，国家卫生研究院(2013年)与Makerere大学一起成立了综合 乌干达H3非洲生物库(IBRH3AU)，一个最先进的样本设施 H3非洲地区基因组研究的收集、加工、储存、检索和分发 财团和其他研究人员。在过去的10年里，IBRH3AU存储了样本 来自H3非洲研究人员和其他研究人员。生物储存库已经 通过建立新冠肺炎生物库，帮助应对新冠肺炎 在提供新冠肺炎诊断试剂盒快速评估样本和利用方面发挥了关键作用 将样本提供给开发新冠肺炎疫苗、疗法和 在乌干达和其他地方的诊断。 随着H3非洲项目接近尾声，乌干达生物库有关键的悬而未决 资金不足的活动。这一行政补充请求的总体目标是确保 关键待完成活动已完成。H3非洲在东非和中非的项目 正在与项目活动敲定将能够完成样品沉积到 生物储存库。通过补充，生物储存库将能够继续处理， 样品的储存和分发给研究人员。此外，还需要增加 用于分析已收集的环境暴露样本的资金和 储存在乌干达生物储存库。由于新冠肺炎，采购设备用于 环境暴露样本的分析被推迟。这份副刊会很有用的 进一步巩固我们的可持续发展计划 办法：(A)乌干达H3非洲生物储存库将利用补编提供的资金 继续支持H3非洲研究人员完成样品运输、加工、储存 和分配。我们将按照以下规定提供质量受控和有保证的生物信息库服务 国际标准化组织20387生物库标准，ISBER最佳实践和标准，与H3非洲一致 数据和生物武器加入政策(DBAC)，(B)除了现有的和已执行的 基因组分析(核酸[DNA和RNA]提取和纯化 血液及其衍生物)、协议、标准操作程序和做法 为收集和存储环境样品而开发和实施 暴露(烟草烟雾、微量元素、全氟烷基和多氟烷基物质、 农药代谢物、霉菌毒素、多环芳烃代谢物、 挥发性有机化合物等)储存的样本还有待分析。《副刊》 将为我们提供一个分析这些样本的机会，并揭示 此设置。(C)继续执行我们的业务计划，以确保 可持续发展，生物库仍将是一种真正的科学变革资源 H3非洲项目。 预期收益： ·确保H3非洲项目活动作为生物库的合乎逻辑的结论 收集并储存剩余的样本。 ·能够分析储存的环境暴露样本，以估计 乌干达问题的严重性 ·继续巩固乌干达生物库的可持续性计划

Summary/Abstract Background: The availability of high quality well annotated biological and environmental specimens for research purposes requires the development of standardised methods for collection, processing, long-term storage, retrieval and distribution of specimens that will enable their future use for research. For this reason, the NIH (in 2013) together with Makerere University established the Integrated Biorepository of H3Africa in Uganda (IBRH3AU), a state-of-the-art facility for sample collection, processing, storage, retrieval and distribution for genomic research for the H3Africa Consortium and other researchers. In the past 10 years, the IBRH3AU has stored samples from both H3Africa researchers and other researchers. The biorepository has been instrumental in the response to COVID-19 through establishment of COVID-19 biobank that was key in providing samples for rapid evaluation of COVID-19 diagnostic Kits and availing samples to the biotech companies developing COVID-19 vaccines, therapeutics and diagnostics in Uganda and beyond. As the H3Africa project draws to the end, the Uganda biorepository has critical pending unfunded activities. The overall goal of this administrative supplement request is to ensure the key pending activities are completed. H3Africa projects within Eastern and Central Africa who are finalising with project activities will be able to complete sample deposition to the biorepository. Through the supplement the biorepository will be able to continue processing, storage and distribution of samples to researchers. In addition, there is need for additional funding to analyse the samples of environmental exposure that have been collected and stored in the Uganda Biorepository. Due to COVID-19, the procurement of equipment for analysis of the environmental exposure samples was delayed. The supplement will be useful in further consolidation of our sustainability plan Approach: (a) The Uganda H3Africa Biorepository will use the funding from the supplement to continue to support the H3Africa researchers to finalise sample shipment, processing, storage and distribution. Quality controlled and assured biorepository services will be offered as per ISO 20387 biobanking standard, ISBER best practices and standards, in line with H3Africa Data and Biospecimen Accession (DBAC) policies, (b) In addition to existing and implemented protocols for genomic analyses (Nucleic acid [DNA and RNA] extraction and purification from blood and its derivatives), protocols, Standard operating procedures and practices have been developed and implemented for collecting and storage of samples for environmental exposures (tobacco smoke, trace elements, perfluoroalkyl and polyfluoroalkyl substances, metabolites of pesticides, toxins from mold, polycyclic aromatic hydrocarbon metabolites, volatile organic compounds, etc.) The stored samples are yet to be analysed. The supplement will provide us with a chance to analyse these samples and reveal the extent of the burden in this setting. (c) Through continued implementation of our business plan to ensure sustainability, the biorepository will remain a true scientific transformatory resource beyond H3Africa project. Expected Benefits: • Ensure logical conclusion of H3Africa project activities as the biorepositories collect and store the remaining samples. • Enable analysis of stored samples of environmental exposures to estimate the magnitude of the problem in Uganda • Continue to consolidate the sustainability plan for the Uganda Biorepository