Intergrated Biorepository of H3Africa Uganda

H3Africa 乌干达综合生物样本库

• 批准号: 10625216
• 负责人: Moses Lutaakome Joloba
• 金额: $ 32.01万
• 依托单位: MAKERERE UNIVERSITY COLLEGE OF HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625216
• 关键词: AIDS/HIV problem; Accreditation; Address; Administrative Supplement; Africa; African; Aromatic Polycyclic Hydrocarbons; Awareness; Bioinformatics; Biological; Biotechnology; Blood; Businesses; COVID-19; COVID-19 diagnostic; COVID-19 vaccine; Central Africa; Collection; Color; Communicable Diseases; Communities; DNA; Data; Deposition; Development; Diabetes Mellitus; Diagnostic; Diagnostic Reagent Kits; Dideoxy Chain Termination DNA Sequencing; Eastern Africa; Ensure; Environment; Environmental Exposure; Enzyme-Linked Immunosorbent Assay; Equipment; Evaluation; Exposure to; Flow Cytometry; Funding; Future; Genes; Genetic; Genomics; Goals; Health; Heart Diseases; Heredity; High Pressure Liquid Chromatography; Human; Hunger; Hypertension; Immunology; Infrastructure; Laboratories; Life Style; Malaria; Malignant Neoplasms; Methods; Microbiology; Molds; Molecular Biology; Nucleic Acids; Outcome; Participant; Patient Recruitments; Pesticides; Policies; Poly-fluoroalkyl substances; Population; Procedures; Process; Protocols documentation; RNA; Research; Research Personnel; Research Project Grants; Research Training; Resources; Retrieval; Sampling; Science; Service provision; Services; Ships; Southern Africa; Specimen; Standardization; Students; System; Temperature; Therapeutic; Time; Tobacco smoke; Toxin; Trace Elements; Training; Uganda; United States National Institutes of Health; Universities; Western Africa; biobank; equipment acquisition; genomic data; repository; response; sample collection; sequencing platform; volatile organic compound

Summary/Abstract Background: The availability of high quality well annotated biological and environmental specimens for research purposes requires the development of standardised methods for collection, processing, long-term storage, retrieval and distribution of specimens that will enable their future use for research. For this reason, the NIH (in 2013) together with Makerere University established the Integrated Biorepository of H3Africa in Uganda (IBRH3AU), a state-of-the-art facility for sample collection, processing, storage, retrieval and distribution for genomic research for the H3Africa Consortium and other researchers. In the past 10 years, the IBRH3AU has stored samples from both H3Africa researchers and other researchers. The biorepository has been instrumental in the response to COVID-19 through establishment of COVID-19 biobank that was key in providing samples for rapid evaluation of COVID-19 diagnostic Kits and availing samples to the biotech companies developing COVID-19 vaccines, therapeutics and diagnostics in Uganda and beyond. As the H3Africa project draws to the end, the Uganda biorepository has critical pending unfunded activities. The overall goal of this administrative supplement request is to ensure the key pending activities are completed. H3Africa projects within Eastern and Central Africa who are finalising with project activities will be able to complete sample deposition to the biorepository. Through the supplement the biorepository will be able to continue processing, storage and distribution of samples to researchers. In addition, there is need for additional funding to analyse the samples of environmental exposure that have been collected and stored in the Uganda Biorepository. Due to COVID-19, the procurement of equipment for analysis of the environmental exposure samples was delayed. The supplement will be useful in further consolidation of our sustainability plan Approach: (a) The Uganda H3Africa Biorepository will use the funding from the supplement to continue to support the H3Africa researchers to finalise sample shipment, processing, storage and distribution. Quality controlled and assured biorepository services will be offered as per ISO 20387 biobanking standard, ISBER best practices and standards, in line with H3Africa Data and Biospecimen Accession (DBAC) policies, (b) In addition to existing and implemented protocols for genomic analyses (Nucleic acid [DNA and RNA] extraction and purification from blood and its derivatives), protocols, Standard operating procedures and practices have been developed and implemented for collecting and storage of samples for environmental exposures (tobacco smoke, trace elements, perfluoroalkyl and polyfluoroalkyl substances, metabolites of pesticides, toxins from mold, polycyclic aromatic hydrocarbon metabolites, volatile organic compounds, etc.) The stored samples are yet to be analysed. The supplement will provide us with a chance to analyse these samples and reveal the extent of the burden in this setting. (c) Through continued implementation of our business plan to ensure sustainability, the biorepository will remain a true scientific transformatory resource beyond H3Africa project. Expected Benefits: • Ensure logical conclusion of H3Africa project activities as the biorepositories collect and store the remaining samples. • Enable analysis of stored samples of environmental exposures to estimate the magnitude of the problem in Uganda • Continue to consolidate the sustainability plan for the Uganda Biorepository

总结/摘要 背景：高质量的生物和环境注释的可用性 用于研究目的的标本需要开发标准化方法， 收集、处理、长期储存、检索和分发标本， 使其未来用于研究。 因此，NIH（2013年）与Makerere大学一起建立了综合研究中心。 乌干达的H3非洲生物储存库（IBRH 3AU），一个最先进的样本储存设施 收集、处理、储存、检索和分发用于H3非洲基因组研究 财团和其他研究人员。在过去的10年里，IBRH 3AU储存了 来自H3非洲研究人员和其他研究人员。生物储藏室已经 通过建立COVID-19生物库，帮助应对COVID-19， 在为快速评估COVID-19诊断试剂盒提供样本方面发挥了关键作用， 向开发COVID-19疫苗、治疗药物和 在乌干达和其他地区进行诊断。 随着H3 Africa项目接近尾声，乌干达生物储存库面临着关键的悬而未决的问题。 无资金活动。这一行政补充请求的总体目标是确保 主要待决活动已完成。H3 Africa在东部和中部非洲的项目， 将能够完成样品沉积， 生物储藏室通过补充，生物储存库将能够继续处理， 储存和分发样本给研究人员。此外，还需要增加 为分析收集的环境接触样本提供资金， 储存在乌干达生物储存库。由于COVID-19，采购设备， 对环境接触样本的分析被推迟。补充将是有益的 为进一步巩固我们的可持续发展计划， 办法：（a）乌干达H3非洲生物储存库将利用补充资金， 继续支持H3 Africa研究人员完成样本运输、处理、储存 和分配。质量控制和有保证的生物储存库服务将按照 ISO 20387生物库标准，ISBER最佳实践和标准，符合H3非洲 数据和生物样本存取（DBAC）政策，（B）除了现有的和已实施的 用于基因组分析的方案（核酸[DNA和RNA]提取和纯化， 血液及其衍生物）、方案、标准操作程序和实践， 为收集和储存环境样品而开发和实施 暴露（烟草烟雾、微量元素、全氟烷基和多氟烷基物质， 农药代谢物，霉菌毒素，多环芳烃代谢物， 挥发性有机化合物等）储存的样本尚待分析。补充剂 将为我们提供一个机会来分析这些样本，并揭示负担的程度， 此设置(c)通过持续实施我们的业务计划， 可持续性，生物储存库将仍然是一个真正的科学转化资源， H3非洲项目。 预期收益： ·确保H3非洲项目活动作为生物储存库的逻辑结论 收集并储存剩余样本。 ·能够分析储存的环境暴露样本，以估计 乌干达问题的严重性 ·继续巩固乌干达生物储藏库的可持续性计划

项目成果

Building blocks for better biorepositories in Africa.

在非洲更好的生物震荡的构建基础。

• DOI: 10.1186/s13073-023-01235-x
• 发表时间: 2023-11-06
• 期刊: GENOME MEDICINE
• 影响因子: 12.3
• 作者: Croxton, Talishiea;Jonathan, Emmanuel;Suleiman, Kareemah;Balogun, Olasinbo;Ozumba, Petronilla;Aloyo, Sharley;Nsubuga, Gideon;Kamulegeya, Rogers;Newton, Lwanga;Mukisa, John;Kader, Mukthar;Damaneite, Vuyo;Nadoma, Sunji;Onyemata, Ezenwa James;Anzaku, Abbas Abel;Nasinghe, Emmanuel;Troyer, Jennifer;Joubert, Bonnie;Beiswanger, Christine;Joloba, Moses;Mayne, Elizabeth;Abimiku, Alash'le
• 通讯作者: Abimiku, Alash'le

Genomic characterization of SARS-CoV-2 from Uganda using MinION nanopore sequencing.

• DOI: 10.1038/s41598-023-47379-z
• 发表时间: 2023-11-22
• 期刊: Scientific reports
• 影响因子: 4.6