Fusion Lumos Orbitrap System for Quantitative Proteomics and Metabolomics Applications
用于定量蛋白质组学和代谢组学应用的 Fusion Lumos Orbitrap 系统
基本信息
- 批准号:10175908
- 负责人:
- 金额:$ 127.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-15 至 2022-07-14
- 项目状态:已结题
- 来源:
- 关键词:AcetylationAlzheimer&aposs DiseaseBiomedical ResearchColoradoDiabetes MellitusDiseaseDissociationDrug Delivery SystemsElectron TransportEquipmentFacultyFundingHealthHumanHybridsImmunologicsLiquid ChromatographyMass Spectrum AnalysisMedicalMetabolic syndromeMethodsMolecular StructureOxidation-ReductionPeptidesPharmacologic SubstancePharmacy SchoolsPost Translational Modification AnalysisPost-Translational Protein ProcessingProteomeProteomicsPublishingRegulationResearchResearch PersonnelResearch Project GrantsResolutionScienceServicesSystemTechnologyUnited States National Institutes of HealthUniversitiesbasedirect applicationexperimental studyextracellular vesiclesimprovedinstrumentinstrumentationinsulin sensitivitylipidomemass spectrometermass spectrometry shared resourcemast cellmetabolomemetabolomicsprogramssmall moleculesuccess
项目摘要
Project Summary
This application is for an Orbitrap Lumos Fusion liquid chromatography mass spectrometer system to be housed
within the Mass Spectrometry Facility at the Skaggs School of Pharmacy and Pharmaceutical Sciences (SSPPS)
at University of Colorado, Anschutz Medical Campus. This instrument will be used to advance several
translational projects that focus on quantitative and discovery-based proteomics and the analysis of post-
translational modifications (PTMs), such as acetylation, with direct application to human health and disease. The
instrument will also be used for metabolomics projects that require high resolving power to improve identification
of unknown compounds. The Users’ projects are currently being conducted on older, Q-TOF instrumentation
that cannot acquire the depth of proteome coverage or resolution required for multiplex proteomics experiments,
PTM analysis, or fine molecular structure elucidation. Hence there is an urgent need for this instrumentation.
The Orbitrap Lumos has several critical features for the intended applications including exceptional sensitivity,
ultra high resolving power, MSn capabilities, electron transfer dissociation (ETD), versatility in quantitative
capabilities, and well-published methods. Dr. David Ross will utilize the Orbitrap Lumos to reveal mechanisms
underlying insulin sensitivity through in-depth, quantitative proteomics and PTM analysis using ETD, with the
potential to impact therapies directed at metabolic syndrome. Dr. Thomas Delong will employ ETD for de novo
hybrid peptide discovery to examine immunological origins of certain diseases, such as diabetes and Alzheimer’s
disease. Dr. Anchordoquy will require the instrument to achieve deep proteome, metabolome, and lipidome
coverage of extracellular vesicles towards improved drug delivery systems. Dr. Brown requires a highly sensitive
and specific redox proteomics approach to understanding regulation of mast cell activation. Finally, the
instrument will be used by Dr. Reisdorph and others to support the identification of small molecules using its
capabilities in multi-stage experiments, MSn, high resolution, and accurate mass. Our long-term objective is to
utilize the Orbitrap Lumos to further develop our programs in acetylomics, PTM quantitation, and metabolomics.
As our Core currently lacks any type of Orbitrap technology, our proteomics and metabolomics services have hit
a plateau; cutting edge instrumentation is required to take the research of our faculty to the next level. The
SSPPS has committed over $500,000 (not including equipment) in the past 5 years towards the improvements
of mass spectrometry facility, which has been supported by SSPPS for almost 20 years. Our objective is to
continue to provide our investigators with a shared mass spectrometry resource to support current and proposed
NIH-funded biomedical research. This Orbitrap is vital to the continued functioning of the facility as a cutting-
edge enterprise and for the success of several potentially high impact research projects.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Nichole A Reisdorph其他文献
Nichole A Reisdorph的其他文献
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{{ truncateString('Nichole A Reisdorph', 18)}}的其他基金
Biomarkers of Insufficient Sleep and Sleepiness
睡眠不足和困倦的生物标志物
- 批准号:
9082143 - 财政年份:2016
- 资助金额:
$ 127.25万 - 项目类别:
Improved Prediction of Response to Asthma Medication Using Small Molecules
使用小分子改进对哮喘药物反应的预测
- 批准号:
8906921 - 财政年份:2014
- 资助金额:
$ 127.25万 - 项目类别:
Sensitive Triple Quadrupole Mass Spectrometer for Translational Research
用于转化研究的灵敏三重四极杆质谱仪
- 批准号:
8247350 - 财政年份:2012
- 资助金额:
$ 127.25万 - 项目类别:
Proteomics & Genomics Hands-On Workshop: From Sample Preparation to Data Analysis
蛋白质组学
- 批准号:
7170444 - 财政年份:2006
- 资助金额:
$ 127.25万 - 项目类别:
Proteomics & Genomics Hands-On Workshop: From Sample Preparation to Data Analysis
蛋白质组学
- 批准号:
7284295 - 财政年份:2006
- 资助金额:
$ 127.25万 - 项目类别:
Proteomics & Genomics Hands-On Workshop: From Sample Preparation to Data Analysis
蛋白质组学
- 批准号:
7677938 - 财政年份:2006
- 资助金额:
$ 127.25万 - 项目类别:
Proteomics & Genomics Hands-On Workshop: From Sample Preparation to Data Analysis
蛋白质组学
- 批准号:
7479578 - 财政年份:2006
- 资助金额:
$ 127.25万 - 项目类别: