Sensitive Triple Quadrupole Mass Spectrometer for Translational Research

描述（由申请人提供）：本申请针对三重四极杆（QQQ）质谱仪系统，目的是在国立犹太健康中心（NJH）的质谱设施内执行转化和基础科学应用。该仪器将用于使用多反应监测（MRM）开发和进行高通量、单重和多重测定，以便定量各种生物流体和复杂样品中的小分子和肽（蛋白质）。在过去的4年里，National Jewish Health已经投入了近100万美元（不包括设备）用于开发最先进的质谱设备;这些技术是NJH超过25名研究人员研究的重要组成部分，他们的长期成功取决于质谱。该提案重点介绍了9个项目，其成功与否具体取决于能否获得所要求的仪器。这些包括定量蛋白质测序、磷酸化事件的定量和临床测定开发。我们的目标是继续为我们的研究人员提供共享的质谱资源，以支持当前和拟议的NIH资助的生物医学研究，NJH为此投入了大量资源。该设施中的仪器已经使用了五年，不再足以满足我们调查人员的需要;我们正在采取措施维持最先进的设施，包括购置新仪器。该提案将具体说明 以下组件：1）对QQQ质谱仪的大量合理需求，2）操作和监督仪器的现有专业知识，3）NIH资助的研究项目的显着增强，4）实用的财务和管理计划，以及5）强有力的机构承诺。NJH质谱设施满足了我们NIH资助的研究人员对最先进技术的巨大需求，以及创新的试点研究和临床诊断的发展。所要求的QQQ将是该设施的重要组成部分。NJH通过25万美元的年度预算支持该设施，包括工资，维护和试剂。作为一个高度专注的机构，我们相信NJH准备在呼吸医学领域提供创新，高影响力的研究。只有当我们的调查人员仍然可以使用尖端技术时，才能实现这一目标。 公共卫生相关性：一个成功的应用程序将通过支持目前从事呼吸和免疫研究的研究人员对公共卫生产生重大影响。该仪器将用于支持有可能验证疾病候选生物标志物的研究，确定药物靶点，并提高我们对几种疾病基本机制的整体知识。

DESCRIPTION (provided by applicant): This application is for a triple quadrupole (QQQ) mass spectrometer system for the purpose of performing translational and basic science applications within the Mass Spectrometry Facility at National Jewish Health (NJH). The instrument will be used to develop and conduct high- throughput, single- and multi-plex assays using multiple reaction monitoring (MRM) in order to quantitate small molecules and peptides (proteins) from a variety of biofluids and complex samples. National Jewish Health has committed almost $1,000,000 (not including equipment) in the past 4 years towards the development of a state-of-the-art mass spectrometry facility; these technologies are an essential component of research for over 25 investigators at NJH whose long-term success is dependent on mass spectrometry. This proposal highlights 9 projects whose success depends specifically on the acquisition of the requested instrument. These include quantitative protein sequencing, quantitation of phosphorylation events, and clinical assay development. Our objective is to continue to provide our investigators with a shared mass spectrometry resource to support current and proposed NIH-funded biomedical research and NJH has committed substantial resources towards this end. Instrumentation in the facility is now five years old and no longer adequate to the needs of our investigators; we are taking steps to maintain a state-of-the-art facility, including acquisition of new instruments. This proposal will specifically demonstrate the following components: 1) a substantial, justified need for a QQQ mass spectrometer, 2) existing expertise to operate and oversee the instrument, 3) significant enhancement of NIH-funded research projects, 4) a practical financial and management plan, and 5) a strong institutional commitment. The NJH mass spectrometry facility addresses the tremendous demand for state-of-the-art technologies by our NIH-funded researchers as well as for innovative pilot studies and the development of clinical diagnostics. The requested QQQ will be an essential component of this facility. NJH supports the facility through an annual budget of $250,000, including salary, maintenance, and reagents. As a highly focused institution, we believe that NJH is poised to deliver innovative, high-impact studies in respiratory medicine. This can only be achieved if cutting edge technologies remain available to our investigators. PUBLIC HEALTH RELEVANCE: A successful application will have a significant impact on public health by supporting researchers who are currently engaged in respiratory and immunological research. The instrument will be used to support studies where there is the potential to validate candidate biomarkers for disease, identify drug targets, and improve our overall knowledge of the basic mechanisms of several diseases.