Biomarkers of Insufficient Sleep and Sleepiness

 描述(申请人提供)：睡眠和觉醒障碍影响5,000万至7,000万美国人，睡眠不足很普遍，超过50%的美国人报告每晚睡眠时间少于7小时。与睡眠不足相关的健康问题包括炎症、抑郁和焦虑、糖尿病、压力、药物滥用、生活质量差、肥胖和工作/驾驶时与疲劳有关的事故。虽然睡眠对整体健康、幸福和公共安全的贡献是公认的，但尚不存在睡眠不足的临床生物标志物。这种生物标记物将作为路边嗜睡的生物标记物(例如，昏昏欲睡的驾驶)、监测工作中的疲劳/执行任务的适合性(例如，交通、军事行动保健)、监测睡眠健康以及用于临床诊断和临床测量 治疗结果。因此，我们设计了一个受控的实验室睡眠不足方案，利用代谢组学来识别睡眠不足的生物标志物。NIH行政副刊授予我们现有的R01“睡眠不足的代谢和认知后果”的初步代谢组学数据为这一建议奠定了基础，并促进了我们提出的发现和有针对性的方法。目前的提案是对2014年睡眠研究学会联合工作组和美国睡眠医学学会报告的主要目标的回应。我们建议确定在睡眠不足期间持续发生的代谢物的变化。我们还将把我们之前研究工作确定的代谢物作为潜在的生物标记物。作为探索性结果，我们将检查睡眠不足时代谢物和认知表现的相关变化。这些拟议的成果支持NIH精密医学倡议和2011年NIH睡眠障碍研究计划，以确定睡眠不足的生物标记物，并使睡眠和昼夜节律研究培训能够在交叉领域加快发现和转换的步伐。

 DESCRIPTION (provided by applicant): Sleep and wakefulness disorders impact 50 to 70 million Americans and insufficient sleep is epidemic with over 50% of Americans reporting less than 7 hours of sleep per night. Health problems associated with insufficient sleep include inflammation, depression and anxiety, diabetes, stress, drug abuse, poor quality of life, obesity, and fatigue related accidents on the job/while driving. While the contribution of sleep to overall health, well-being, and public safety is recognized, no established clinical biomarkers of sleep deficiency exist. Such biomarkers would have utility as road-side biomarkers of sleepiness (e.g., drowsy driving), monitoring on the job fatigue/fitness for duty (e.g., transportation, military ops health care), monitoring sleep health, as well as for clinical diagnostics and measures of clinical treatment outcomes. Thus, we designed a controlled laboratory insufficient sleep protocol utilizing metabolomics to identify biomarkers of insufficient sleep. Preliminary metabolomics data from our NIH Administrative Supplement awarded to our existing R01 "Metabolic and Cognitive Consequences of Sleep Loss" set the stage for this proposal and facilitated our proposed discovery and targeted approaches. The current proposal is responsive to key goals of the 2014 Joint Task Force of the Sleep Research Society and American Academy of Sleep Medicine report. We propose to identify changes in metabolites that consistently occur during insufficient sleep. We will also target metabolites identified by our previous research efforts as potential biomarkers. As an exploratory outcome we will examine associated changes in metabolites and cognitive performance during insufficient sleep. These proposed outcomes support the NIH Precision Medicine Initiative and the 2011 NIH Sleep Disorders Research Plan to identify biomarkers of sleep deficiency and enabling sleep and circadian research training in cross-cutting domains to accelerate the pace of discovery and translation.