NARMS Retail Food Surveillance in Ohio

总结 抗生素耐药性对人类和动物健康的威胁日益严重。在美国 仅每年就有超过23，000人死于抗生素耐药性感染， 有机体耐药细菌病原体暴露的一个常见来源是食物，特别是新鲜的 零售肉类产品。零售肉制品中存在的大多数食源性细菌病原体也 在生产过程中寄生于食用动物的胃肠道。肉类污染 产品发生在屠宰过程中，当胃肠道内容物，包括粪便细菌， 污染尸体尽管食品安全有所改善，但食源性疾病的负担 在过去的十年中保持相对不变，每年影响美国六分之一的人。 为协助解决这个重要的公共卫生问题，我们建议采取以下措施 具体目标：1）促进国家对食源性病原体和指标的监测 细菌作为NARMS零售食品监测计划的一部分，2）支持爆发 通过提供现场流行病学和实验室支持调查食源性疾病， 其他机构在俄亥俄州，和3）进行实地研究，以了解水库的人畜共患病 食源性传播和耐药性病原体的分子流行病学 和指示细菌。 为了实现这些具体目标，我们将作为一个NARMS零售肉类和海鲜监督 项目贡献实验室作为一个大型的集群监测点，我们将对零售家禽进行采样， 牛肉，猪肉，海鲜从零售店内都大都市和农村俄亥俄州。四十八 每月将对样本进行特定病原体和指示细菌培养。所得 分离株将进一步表征，并每月运送给FDA进行抗菌药物敏感性检测 测试，在我们的培养物收藏中保留重复分离株。我们希望成功地 每年对528包零售牛肉、猪肉、家禽和海鲜进行抽样，这将有助于跟踪 食源性致病菌及指示菌耐药性现状 在零售肉类和海鲜中发现，但也将影响未来的FDA政策和法规， 监测行动和干预措施的成功。除了我们对零售业的贡献， 食品监督计划，作为NARMS参与实验室，我们将提供支持， 根据需要，在俄亥俄州和附近各州开展食源性疾病暴发调查。

Summary Antimicrobial resistance is an increasingly critical threat to human and animal health. In the US alone, >23,000 individuals die each year because of infections with antimicrobial resistant organisms. A common source of resistant bacterial pathogen exposure is food, in particular fresh retail meat products. Most foodborne bacterial pathogens present in retail meat products also colonize the gastrointestinal tract of food animals during production. Contamination of meat products occurs during the slaughter process when GI contents, including fecal bacteria, contaminate the carcass. Despite improved food safety, the burden of foodborne illness has remained relatively unchanged for the past decade, affecting 1 in 6 individuals in the US annually. To help address this important public health problem, we propose to accomplish the following specific aims: 1) Contribute to national surveillance of foodborne pathogens and indicator bacteria as part of the NARMS retail food surveillance program, 2) Support outbreak investigations of foodborne disease by providing field epidemiology and laboratory support to other agencies in Ohio, and 3) Conduct field research to understand the reservoirs for zoonotic foodborne transmission, and the molecular epidemiology of antimicrobial resistant pathogens and indicator bacteria in fresh retail meat products. To accomplish these specific aims, we will serve as a NARMS retail meat and seafood surveillance project contributing laboratory. As a large cluster surveillance site, we will sample retail poultry, beef, pork, and seafood from retail stores within both metropolitan and rural Ohio. Forty-eight monthly samples will be cultured for specific pathogens and indicator bacteria. The resulting isolates will be further characterized and shipped monthly to FDA for antimicrobial susceptibility testing, with duplicate isolates maintained in our culture collection. We expect to successfully sample 528 packages of retail beef, pork, poultry, and seafood annually that will help to track the current state of antimicrobial resistance among foodborne pathogens and indicator bacteria found in retail meat and seafood but will also impact future FDA policy and regulations and monitor the success of actions and interventions. In addition to our contributions to the retail food surveillance program, as a NARMS participating laboratory we will be available to support foodborne disease outbreak investigations in Ohio and nearby states as needed.