Q-band Upgrade to an X-band Pulsed EPR spectrometer
Q-band Upgrade to an X-band Pulsed EPR spectrometer
批准号:
10177244
负责人:
ROBERT Guy GRIFFIN
金额:
$92.02万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-07-15 至 2024-07-14
关键词:
2,4-DinitrophenolAddressAnesthesia proceduresAreaBacterial ProteinsBiochemistryBiologicalBostonChemistryCollagenCommunicable DiseasesComplementComplexElectron Nuclear Double ResonanceElectronsEnzymesHealthHumanImmunityInvestigationMedicalMembraneMembrane ProteinsMethodsMicroscopeMinorMolybdoferredoxinMotivationNitrogenaseNuclear Magnetic ResonancePhysiologic pulsePolysaccharidesProteinsRainResearchResearch PersonnelResearch Project GrantsResolutionStructureViral ProteinsX-Ray Crystallographyimprovedinstrumentmedical schoolsmetalloenzymemultidisciplinarynervous system disorderstructural biology
中文摘要
总结。
英文摘要
Summary.
A continuous wave and pulsed electron paramagnetic (EPR) spectrometer operating at 34 GHz
(Q-band) is requested by investigators at MIT Chemistry, Harvard Medical School, Harvard
Chemistry and Brandeis Biochemistry. The instrument’s capabilities include pulsed ENDOR and
ELDOR. The primary motivation for the Q-band instrument is the much improved sensitivity and
resolution available at 34 GHz as compared to our current X-band (9 GHz) spectrometer. This
improved sensitivity and resolution will permit us to address many problems which at the moment
are beyond our scientific reach. The instrument will complement high-end X-ray crystallography,
nuclear magnetic resonance (NMR), and cryoelectron microscope (cryoEM) capabilities in the
Boston area to enable multidisciplinary structural biology and mechanistic biochemistry
investigations of questions that are of great biological and medical importance. Research projects
from six major user groups (Griffin, Nocera, Nolan, Pandelia, Raines, and Suess) and those from
another three minor groups (Betley Keissling, and Wagner) are included in the proposal.
The instrument will advance the following health-related research: 1) ENDOR and ESSEM studies
of reactive intermediates in radical SAM enzymes, metalloenzymes involved in human immunity, and
polynuclear Fe complexes that mimic the nitrogenase FeMo-cofactor; 2) pulsed EPR/DNP methods
aimed at developing methods to dynamically polarize proteins at high fields and 3) DEER studies
of membrane and soluble proteins, collagen, and polysaccharide secondary structure;. We
anticipate that further projects targeting membrane proteins important for example in neurological
disease and anesthesia; and viral and bacterial proteins important in infectious disease will appear
during the lifetime of the spectrometer.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Community Engagement
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批准号:10224252
-
项目类别:
-
资助金额:$0.82万
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财政年份:2019
-
负责人:ROBERT Guy GRIFFIN
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依托单位:
MIT/Harvard Center for Magnetic Resonance
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批准号:10224248
-
项目类别:
-
资助金额:$104.43万
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财政年份:2019
-
负责人:ROBERT Guy GRIFFIN
-
依托单位:
Cryoprobe repairs and amplifier replacement for the 800 and 750 MHz spectrometers
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批准号:10400474
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项目类别:
-
资助金额:$8.27万
-
财政年份:2019
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负责人:ROBERT Guy GRIFFIN
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依托单位:
TR&D 4 -- Advanced Magic Angle Spinning NMR methods
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批准号:10224251
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项目类别:
-
资助金额:$3.0万
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财政年份:2019
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Admin & Mgmt
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批准号:10224249
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项目类别:
-
资助金额:$95.0万
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财政年份:2019
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负责人:ROBERT Guy GRIFFIN
-
依托单位:
Solid State NMR Studies of Amyloid Proteins
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批准号:10446323
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项目类别:
-
资助金额:$70.95万
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财政年份:2017
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Solid State NMR Studies of Amyloid Proteins
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批准号:10687158
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项目类别:
-
资助金额:$70.85万
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财政年份:2017
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负责人:ROBERT Guy GRIFFIN
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依托单位:
U.S.-Canada Winter School on Biomolecular Solid State NMR
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批准号:9398504
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项目类别:
-
资助金额:$1.0万
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财政年份:2017
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Solid State NMR Studies of Amyloid Proteins
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批准号:9366854
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项目类别:
-
资助金额:$305.77万
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财政年份:2017
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Console for an 800/89 DNP Spectrometer
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批准号:8448486
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项目类别:
-
资助金额:$53.9万
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财政年份:2013
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负责人:ROBERT Guy GRIFFIN
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依托单位:
3rd U.S.-Canada Winter School on Biomolecular Solid State NMR
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批准号:8459165
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项目类别:
-
资助金额:$1.0万
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财政年份:2012
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负责人:ROBERT Guy GRIFFIN
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依托单位:
800 MHz Low Temperature Magic Angle Spinning NMR Probe.
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批准号:7794177
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项目类别:
-
资助金额:$30.0万
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财政年份:2009
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负责人:ROBERT Guy GRIFFIN
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依托单位:
2nd U.S.-Canada Winter School on Biomolecular Solid State NMR
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批准号:7806213
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项目类别:
-
资助金额:$0.5万
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财政年份:2009
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Acquisition of a 900 MHz NMR Spectrometer
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批准号:6548308
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项目类别:
-
资助金额:$531.13万
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财政年份:2002
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Acquisition of a 900 MHz NMR Spectrometer
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批准号:7088731
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项目类别:
-
资助金额:$36.06万
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财政年份:2002
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Acquisition of a 900 MHz NMR Spectrometer
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批准号:6604943
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项目类别:
-
资助金额:$34.38万
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财政年份:2002
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Acquisition of a 900 MHz NMR Spectrometer
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批准号:6901945
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项目类别:
-
资助金额:$36.05万
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财政年份:2002
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负责人:ROBERT Guy GRIFFIN
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依托单位:
Acquisition of a 900 MHz NMR Spectrometer
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批准号:6765165
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项目类别:
-
资助金额:$35.2万
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财政年份:2002
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负责人:ROBERT Guy GRIFFIN
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依托单位:
SOLID STATE NMR FOR AMYLOID STRUCTURE DETERMINATION
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批准号:6469200
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项目类别:
-
资助金额:$21.7万
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财政年份:2001
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负责人:ROBERT Guy GRIFFIN
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依托单位:
SOLID STATE NMR FOR AMYLOID STRUCTURE DETERMINATION
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批准号:6345897
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项目类别:
-
资助金额:$21.7万
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财政年份:2000
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负责人:ROBERT Guy GRIFFIN
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依托单位:
海外基金