Console for an 800/89 DNP Spectrometer

800/89 DNP 光谱仪控制台

• 批准号: 8448486
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 53.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2014-06-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448486
• 关键词: Amyloid Fibrils; Amyloid Proteins; Amyloidosis; Area; Bacteriorhodopsins; Biological; Boston; Communities; Dialysis procedure; Funding; Funding Applicant; Future; Gases; Grant; Investigation; Lipid Bilayers; M2 protein; Magic; Membrane; Membrane Proteins; North America; Nuclear; Phosphatidylinositols; Phosphotransferases; Proteins; Research; Research Project Grants; SH3 Domains; Sampling; Scientist; System; Techniques; Temperature; Testing; Vacuum; Vesicle; Voltage-Dependent Anion Channel; beta-2 Microglobulin; cold temperature; influenzavirus; innovative technologies; instrument; instrumentation; nanodisk; prototype; research study; solid state nuclear magnetic resonance; structural biology

DESCRIPTION (provided by applicant): Funding is requested for the purchase of three essential items that will be used with a 800/89 magnet: (1) a spectrometer console, (2) room temperature shims and (3) a magic angle spinning (MAS) probe with a 1.3 mm stator rotor system. The magnet was delivered in February and is presently at field. In the immediate future the spectrometer will be operated as a high field MAS NMR with low temperature capabilities. However, in the long term it will be converted to an 800 MHz/527 GHz DNP instrument. With funds from another grant we purchased a probe capable of performing experiments in the 90-300 K range and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum jacketed dewar, sample eject capabilities, and includes a waveguide for dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (6) phosphatidyl-inositol-3-kinase SH3 domain (PI3- SH3), (7) beta-2-microglobulin associated with dialysis related amyloidosis, and (8) the CsgA protein, from Curli. In addition, this will be a prototype instrument performing DNP experiments at 800/527 and will (9) be used to test new biradical polarizing agents, (10) develop high field 17O MAS experiments and (11) new techniques for dipole recoupling at high ?r/2?. The 800/89 will be part of a cluster of instrumentation that serves a community of scientist performing high field biological NMR in the Boston area and North America. It will alleviate a pressing need for additional capabilities for low temperature and high field DNP/ MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not available elsewhere. For example, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available worldwide and the addition of a 527 GHz gyrotron will make the system a unique DNP spectrometer in North America. It will therefore fill an important void in structural biology.

描述（由申请人提供）：申请资金用于购买将与800/89磁体一起使用的三个基本项目：（1）光谱仪控制台，（2）室温恒温器和（3）带有1.3 mm定子转子系统的魔角旋转（MAS）探头。磁铁于2月交付，目前正在现场。在不久的将来，光谱仪将作为具有低温能力的高场MAS NMR操作。然而，从长远来看，它将被转换为800 MHz/527 GHz DNP仪器。利用另一笔赠款的资金，我们购买了一个能够在90-300 K范围内进行实验的探针，并将用于检查冷冻保护膜蛋白和淀粉样蛋白样品中的各种温度依赖性现象。该探头有一个真空夹套杜瓦，样品弹射能力，并包括一个在527 GHz的动态核极化（DNP）实验的波导。研究项目包括以下膜和淀粉样蛋白的研究：（1）天然膜中的细菌视紫红质（bR）光循环中间体和（2）纳米盘，（3）脂质双层中的电压依赖性阴离子通道（VDAC），（4）来自流感病毒的M2蛋白，（5）形成蛋白质膜的气泡，以及由（6）磷脂酰肌醇-3-激酶SH 3结构域（PI 3-SH 3）、（7）与透析相关的淀粉样变性相关的β-2-微球蛋白和（8）CsgA蛋白形成的淀粉样原纤维，来自Curli。此外，这将是一个原型仪器进行DNP实验在800/527，并将（9）用于测试新的双自由基极化剂，（10）开发高场17 O MAS实验和（11）新技术的偶极再耦合在高？r/2？800/89将成为一个仪器集群的一部分，为科学家社区服务。 在波士顿地区和北美进行高场生物核磁共振。它将缓解对低温和高场DNP/ MAS实验额外能力的迫切需求。此外，该仪器将对研究产生重大影响，因为它将采用其他地方没有的一些创新技术。例如，LT-MAS探头与800/89的组合将成为全球为数不多的宽孔低温固态NMR仪器之一，而527 GHz回旋管的增加将使该系统成为北美唯一的DNP光谱仪。因此，它将填补结构生物学的一个重要空白。