Console for an 800/89 DNP Spectrometer

描述（由申请人提供）：申请资金用于购买将与800/89磁铁一起使用的三个基本项目：(1)光谱仪控制台，(2)室温垫片和(3)带1.3毫米定子转子系统的魔角旋转（MAS）探头。该磁体于2月份交付，目前正在现场。在不久的将来，光谱仪将作为具有低温能力的高场MAS核磁共振运行。然而，从长远来看，它将被转换为800 MHz/527 GHz DNP仪器。在另一笔拨款的资助下，我们购买了一个能够在90-300 K范围内进行实验的探针，并将用于检查低温保护膜蛋白和淀粉样蛋白样品中的各种温度依赖现象。该探针具有真空夹套杜瓦瓶，样品弹射能力，并包括一个用于527 GHz动态核极化（DNP）实验的波导。研究项目包括以下膜和淀粉样蛋白的研究：(1)天然膜中的细菌紫质（bR）光循环中间体和(2)纳米圆盘，(3)脂质双层中的电压依赖性负离子通道（VDAC），(4)流感病毒中的M2蛋白，(5)形成蛋白膜的气体囊泡，以及由(6)磷脂酰肌醇-3-激酶SH3结构域（PI3- SH3）形成的淀粉样蛋白原纤维，(7)与透析相关淀粉样变性相关的β -2微球蛋白，(8)来自Curli的CsgA蛋白。此外，这将是一个在800/527下进行DNP实验的原型仪器，并将(9)用于测试新的双基极化剂，（10）开发高场17O MAS实验和（11）在高r/2下偶极重偶联的新技术。800/89将成为服务于科学家群体的一组仪器的一部分

DESCRIPTION (provided by applicant): Funding is requested for the purchase of three essential items that will be used with a 800/89 magnet: (1) a spectrometer console, (2) room temperature shims and (3) a magic angle spinning (MAS) probe with a 1.3 mm stator rotor system. The magnet was delivered in February and is presently at field. In the immediate future the spectrometer will be operated as a high field MAS NMR with low temperature capabilities. However, in the long term it will be converted to an 800 MHz/527 GHz DNP instrument. With funds from another grant we purchased a probe capable of performing experiments in the 90-300 K range and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum jacketed dewar, sample eject capabilities, and includes a waveguide for dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (6) phosphatidyl-inositol-3-kinase SH3 domain (PI3- SH3), (7) beta-2-microglobulin associated with dialysis related amyloidosis, and (8) the CsgA protein, from Curli. In addition, this will be a prototype instrument performing DNP experiments at 800/527 and will (9) be used to test new biradical polarizing agents, (10) develop high field 17O MAS experiments and (11) new techniques for dipole recoupling at high ?r/2?. The 800/89 will be part of a cluster of instrumentation that serves a community of scientist performing high field biological NMR in the Boston area and North America. It will alleviate a pressing need for additional capabilities for low temperature and high field DNP/ MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not available elsewhere. For example, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available worldwide and the addition of a 527 GHz gyrotron will make the system a unique DNP spectrometer in North America. It will therefore fill an important void in structural biology.