Console for an 800/89 DNP Spectrometer

申请者描述(由申请者提供)：申请资金用于购买将与800/89磁体一起使用的三项基本物品：(1)光谱仪控制台，(2)室温垫片和(3)带有1.3 mm定子转子系统的魔角旋转(MAS)探头。该磁铁已于2月份交付，目前已投入使用。在不久的将来，该光谱仪将作为具有低温能力的高场MAS核磁共振装置运行。然而，从长远来看，它将转换为800 MHz/527 GHz DNP仪器。用另一笔赠款的资金，我们购买了一个能够在90-300K范围内进行实验的探测器，并将用于研究低温保护的膜蛋白和淀粉样蛋白样品中的各种温度依赖现象。该探测器具有真空外壳杜瓦，样品弹出能力，并包括用于527 GHz动态核极化(DNP)实验的波导。研究项目包括对膜和淀粉样蛋白的研究：(1)天然膜和纳米盘中的细菌视紫红质(BR)光循环中间体，(3)脂双层中的电压依赖性阴离子通道(VDAC)，(4)流感病毒的M2蛋白，(5)形成蛋白质膜的气泡，以及(6)磷脂酰肌醇-3-激酶SH3结构域(PI3-SH3)形成的淀粉样纤维，(7)与透析相关的淀粉样变性相关的β-2-微球蛋白，以及(8)来自Curli的CsgA蛋白。此外，这将是一个在800/527下进行DNP实验的原型仪器，将(9)用于测试新的双自由基极化剂，(10)发展高场17O MAS实验和(11)在高R/2？下进行偶极再耦合的新技术。800/将是为科学家社区服务的仪器集群的一部分 在波士顿地区和北美进行高场生物核磁共振。它将缓解低温和高场DNP/MAS实验对额外能力的迫切需求。此外，该仪器将对研究产生重大影响，因为它将采用许多其他地方没有的创新技术。例如，LT-MAS探头与800/89的组合将是全球范围内为数不多的宽带低温固态核磁共振仪器之一，而527 GHz回旋加速器的加入将使该系统成为北美独一无二的DNP光谱仪。因此，它将填补结构生物学中的一个重要空白。

DESCRIPTION (provided by applicant): Funding is requested for the purchase of three essential items that will be used with a 800/89 magnet: (1) a spectrometer console, (2) room temperature shims and (3) a magic angle spinning (MAS) probe with a 1.3 mm stator rotor system. The magnet was delivered in February and is presently at field. In the immediate future the spectrometer will be operated as a high field MAS NMR with low temperature capabilities. However, in the long term it will be converted to an 800 MHz/527 GHz DNP instrument. With funds from another grant we purchased a probe capable of performing experiments in the 90-300 K range and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum jacketed dewar, sample eject capabilities, and includes a waveguide for dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (6) phosphatidyl-inositol-3-kinase SH3 domain (PI3- SH3), (7) beta-2-microglobulin associated with dialysis related amyloidosis, and (8) the CsgA protein, from Curli. In addition, this will be a prototype instrument performing DNP experiments at 800/527 and will (9) be used to test new biradical polarizing agents, (10) develop high field 17O MAS experiments and (11) new techniques for dipole recoupling at high ?r/2?. The 800/89 will be part of a cluster of instrumentation that serves a community of scientist performing high field biological NMR in the Boston area and North America. It will alleviate a pressing need for additional capabilities for low temperature and high field DNP/ MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not available elsewhere. For example, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available worldwide and the addition of a 527 GHz gyrotron will make the system a unique DNP spectrometer in North America. It will therefore fill an important void in structural biology.