Molecular Imaging for Detection of Synthetic Biology Circuits, Oscillators and Toggle Switches in Regenerative Medicine
用于检测再生医学中的合成生物学电路、振荡器和拨动开关的分子成像
基本信息
- 批准号:10176612
- 负责人:
- 金额:$ 33.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-07-01 至 2023-05-31
- 项目状态:已结题
- 来源:
- 关键词:AdipocytesAmplifiersAnimalsBiologicalBiological ProductsBiomedical EngineeringBrainCalciumCatfishCell Culture SystemCell Differentiation processCell Fate ControlCell membraneCell surfaceCellsChronicClinical PathsCultured CellsDataDevicesDiabetes MellitusDiagnosticDiseaseDopamineElectromagnetic FieldsElectromagneticsElectronicsEngineered GeneEngineeringEukaryotic CellFOS geneFutureGene ExpressionGene FamilyGenesGeneticGlassGoalsHerpesvirus 1Hormone secretionHormonesHumanImageImmediate-Early GenesIn SituInduced pluripotent stem cell derived neuronsInjectionsInsulinLab-On-A-ChipsLeadLigand BindingMagnetic Resonance ImagingMalignant NeoplasmsMammalian CellMembrane ProteinsMicrofluidic MicrochipsMicroscopyMoldsMonitorMusNeuronsNeurotransmittersNuclearOocytesOptical reporterOutcomeParkinson DiseasePathway interactionsPatternPeriodicityPharmaceutical PreparationsPopulationPositron-Emission TomographyProcessProductionProteinsPumpRegenerative MedicineReporter GenesRodentSpecificitySurfaceSystemTechnologyTestingTherapeuticThyrotropinTimeTissuesTranslatingTransplantationVisualizationWireless Technologycancer therapyclinical translationcontrolled releasecytokinedeoxyribonucleoside kinasesdesignferriteglucagon-like peptide 1imaging detectionin vivoin vivo imaginginnovationmembermicrodevicemicroorganismmolecular imagingnanoparticleneurotropicnovelpluripotencypromoterremote controlstem cell fatestem cell therapystem cellssuccesssynthetic biologytherapeutic genethymidine kinase 1tissue regenerationtool
项目摘要
Harnessing the power of therapeutic stem cells holds great promises for the discovery of new treatments to
many chronic and uncured diseases. Despite few milestone studies, the overall success has been limited.
Studies around the world showed that it is safe to use such cells, however, those cells must be first engineered
so they can preformed the desired task, similar to the way that electronic component are engineered for
preforming computational tasks. In synthetic biology, much like in electronics, we can use biological
components to build a circuit that can preform a very specific function. Instead of using electronic parts we can
used biological parts or “bio-parts” that interact with the outmost specificity one with another. Here we seek to
use three “bio-parts” that will be sufficient to control stem cell activity and fate in situ. This is extremely
important because to date, there is no way to remote control cells, inside the body, without invasively
penetrating the body tissue.
To that end, we will construct a biological circuit inside stem cells. For the “switch” we will use a novel protein,
encoded by a gene that was discovered in our lab. This novel protein can sense and respond to an external
electromagnetic field, i.e., can be switch on and off by a static magnet or an electronic device. Once it is
activated it lead to release of calcium. A calcium sensitive promoter is used as an “amplifier”. The third bio-part
is a reporter gene that we will use for visualization of the activity and in the future can be replace by a
therapeutic gene.
In the first aim we will establish a cell culture system that can express all the bio-parts of the “device” in stem
cells. Specifically in iPSCs-derived neurons and adipocytes derived stem cells (ADSCs). In the second Aim we
will test two modes of cell activation. The first one is with ferromagnetic (not to be confused with paramagnetic)
nanoparticles (FMNPs). Those FMNPs will be functionalized with ligands that bind to the stem cell membrane.
This will result in continues activation (“off”→”on”) of genes. This is relevant to cases where we need to induce
cell differentiation either to specific linage or even to reverse pluripotency. The second mode of activation is
oscillations, induced by an electromagnet, that can we alternately switch on and off for brief time periods. This
can create an oscillatory pattern that can result in cyclic production of metabolites, hormones and drugs. This
is relevant, for example, for disease such as diabetes where it can replace the daily insulin injection or in
cancer where are controlled drug release is required. In the third Aim, we will transplant the bioengineered
stem cells in the rodent brain and will monitor both the Toggle switch and the oscillator in vivo using a reporter
gene engineered for both MRI and PET. Thus, this innovative study is a unique approach to transition synthetic
biology strategies from microorganism to mammalian system with clear path for clinical translation.
利用治疗性干细胞的力量为发现新的治疗方法带来了巨大的希望
项目成果
期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Development of a Synthetic Biosensor for Chemical Exchange MRI Utilizing In Silico Optimized Peptides.
利用计算机优化的肽开发用于化学交换 MRI 的合成生物传感器。
- DOI:10.1101/2023.03.08.531737
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Fillion,AdamJ;Bricco,AlexanderR;Lee,HarveyD;Korenchan,David;Farrar,ChristianT;Gilad,AssafA
- 通讯作者:Gilad,AssafA
Bioengineering of Genetically Encoded Gene Promoter Repressed by the Flavonoid Apigenin for Constructing Intracellular Sensor for Molecular Events.
- DOI:10.3390/bios11050137
- 发表时间:2021-04-28
- 期刊:
- 影响因子:0
- 作者:Desmet NM;Dhusia K;Qi W;Doseff AI;Bhattacharya S;Gilad AA
- 通讯作者:Gilad AA
Proposed three-phenylalanine motif involved in magnetoreception signalling of an Actinopterygii protein expressed in mammalian cells.
- DOI:10.1098/rsob.230019
- 发表时间:2023-11
- 期刊:
- 影响因子:5.8
- 作者:
- 通讯作者:
A Novel Protein for the Bioremediation of Gadolinium Waste.
用于钆废物生物修复的新型蛋白质。
- DOI:10.1101/2023.01.05.522788
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Lee,HarveyD;Grady,ConnorJ;Krell,Katie;Strebeck,Cooper;Good,NathanM;Martinez-Gomez,NCecilia;Gilad,AssafA
- 通讯作者:Gilad,AssafA
Non-invasive neuromodulation using rTMS and the electromagnetic-perceptive gene (EPG) facilitates plasticity after nerve injury.
- DOI:10.1016/j.brs.2020.10.006
- 发表时间:2020-11
- 期刊:
- 影响因子:7.7
- 作者:Cywiak C;Ashbaugh RC;Metto AC;Udpa L;Qian C;Gilad AA;Reimers M;Zhong M;Pelled G
- 通讯作者:Pelled G
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Assaf A Gilad其他文献
Cardiac CEST-MRI for tracking stem cell survival and determining the role of CXCL2
- DOI:
10.1186/1532-429x-18-s1-p262 - 发表时间:
2016-01-27 - 期刊:
- 影响因子:
- 作者:
Lina Alon;Dara Kraitchman;Michael Schär;Angel Cortez;Nirbhay N Yadav;Judy Cook;Peter V Johnston;Rebecca Krimins;Michael T McMahon;Peter van Zijl;Jeff W Bulte;Assaf A Gilad - 通讯作者:
Assaf A Gilad
Assaf A Gilad的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Assaf A Gilad', 18)}}的其他基金
Semi-synthetic, magneto-photonic circuit for non-invasive control of cellular function
用于非侵入性控制细胞功能的半合成磁光子电路
- 批准号:
10277517 - 财政年份:2021
- 资助金额:
$ 33.24万 - 项目类别:
Bioengineering a novel electromagnetic perspective gene as a tool for wireless control of excitable cells
生物工程新型电磁透视基因作为无线控制可兴奋细胞的工具
- 批准号:
10200903 - 财政年份:2017
- 资助金额:
$ 33.24万 - 项目类别:
Bioengineering a novel electromagnetic perspective gene as a tool for wireless control of excitable cells
生物工程新型电磁透视基因作为无线控制可兴奋细胞的工具
- 批准号:
9381612 - 财政年份:2017
- 资助金额:
$ 33.24万 - 项目类别:
Adaptive control of epileptic seizures using a genetically encoded sensor
使用基因编码传感器自适应控制癫痫发作
- 批准号:
8733830 - 财政年份:2012
- 资助金额:
$ 33.24万 - 项目类别:
Adaptive control of epileptic seizures using a genetically encoded sensor
使用基因编码传感器自适应控制癫痫发作
- 批准号:
8445212 - 财政年份:2012
- 资助金额:
$ 33.24万 - 项目类别:
Adaptive control of epileptic seizures using a genetically encoded sensor
使用基因编码传感器自适应控制癫痫发作
- 批准号:
8599497 - 财政年份:2012
- 资助金额:
$ 33.24万 - 项目类别:
Adaptive control of epileptic seizures using a genetically encoded sensor
使用基因编码传感器自适应控制癫痫发作
- 批准号:
8333669 - 财政年份:2012
- 资助金额:
$ 33.24万 - 项目类别:
Adaptive control of epileptic seizures using a genetically encoded sensor
使用基因编码传感器自适应控制癫痫发作
- 批准号:
8789397 - 财政年份:2012
- 资助金额:
$ 33.24万 - 项目类别:
Imaging of gene delivery in the central nervous system
中枢神经系统基因传递的成像
- 批准号:
7659274 - 财政年份:2009
- 资助金额:
$ 33.24万 - 项目类别:
Imaging of gene delivery in the central nervous system
中枢神经系统基因传递的成像
- 批准号:
7858506 - 财政年份:2009
- 资助金额:
$ 33.24万 - 项目类别:
相似海外基金
SBIR Phase II: Thermally-optimized power amplifiers for next-generation telecommunication and radar
SBIR 第二阶段:用于下一代电信和雷达的热优化功率放大器
- 批准号:
2335504 - 财政年份:2024
- 资助金额:
$ 33.24万 - 项目类别:
Cooperative Agreement
Interferometric and Multiband optical Parametric Amplifiers for Communications (IMPAC)
用于通信的干涉式和多频带光学参量放大器 (IMPAC)
- 批准号:
EP/X031918/1 - 财政年份:2024
- 资助金额:
$ 33.24万 - 项目类别:
Fellowship
Josephson Parametric Amplifiers using CVD graphene junctions
使用 CVD 石墨烯结的约瑟夫森参量放大器
- 批准号:
EP/Y003152/1 - 财政年份:2024
- 资助金额:
$ 33.24万 - 项目类别:
Research Grant
Semiconductor-based Terahertz Traveling Wave Amplifiers for Monolithic Integration
用于单片集成的半导体太赫兹行波放大器
- 批准号:
2329940 - 财政年份:2023
- 资助金额:
$ 33.24万 - 项目类别:
Standard Grant
OPTIME-PA: Optimal MMIC Design of E-Band Power Amplifiers for Satcom using Dedicated Measurements and Non-Linear Modelling
OPTIME-PA:使用专用测量和非线性建模的卫星通信 E 频段功率放大器的最佳 MMIC 设计
- 批准号:
10075892 - 财政年份:2023
- 资助金额:
$ 33.24万 - 项目类别:
Collaborative R&D
Optical Glass Amplifiers for High Capacity Networks
用于高容量网络的光学玻璃放大器
- 批准号:
538379-2018 - 财政年份:2022
- 资助金额:
$ 33.24万 - 项目类别:
Collaborative Research and Development Grants
Investigating the function of ZU5 domain-containing proteins as amplifiers of caspase activation
研究含有 ZU5 结构域的蛋白质作为 caspase 激活放大器的功能
- 批准号:
10681326 - 财政年份:2022
- 资助金额:
$ 33.24万 - 项目类别:
Investigating the function of ZU5 domain-containing proteins as amplifiers of caspase activation
研究含有 ZU5 结构域的蛋白质作为 caspase 激活放大器的功能
- 批准号:
10621402 - 财政年份:2022
- 资助金额:
$ 33.24万 - 项目类别:
Broadband Digital Doherty Amplifiers for Sub-6 GHz 5G wireless Applications
适用于 6 GHz 以下 5G 无线应用的宽带数字 Doherty 放大器
- 批准号:
573452-2022 - 财政年份:2022
- 资助金额:
$ 33.24万 - 项目类别:
Alliance Grants
TALENT – Tapered AmpLifiErs for quaNtum Technologies
人才 — 量子技术的锥形放大器
- 批准号:
10032436 - 财政年份:2022
- 资助金额:
$ 33.24万 - 项目类别:
Collaborative R&D