Mechanisms of SPW-R sequencing and termination

SPW-R 测序和终止机制

• 批准号: 10202752
• 负责人: GYORGY BUZSAKI
• 金额: $ 46.09万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-25 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10202752
• 关键词: Apical; Data; Electrophysiology (science); Event; Hippocampus (Brain); Image; Interneurons; Light; Measures; Medial; Mediating; Memory; Methods; Neurosciences; Optical Methods; Optics; Pattern; Play; Positioning Attribute; Probability; Process; Pyramidal Cells; Role; Signal Transduction; Silicon; Site; Source; Testing; cell type; density; dentate gyrus; experimental study; extracellular; independent component analysis; neural network; operation; optogenetics; recruit; simulation; two-photon; voltage

The memory function of SPW-Rs depends critically on rapid sequential replay of memory items (e.g. spatial positions along an explored path). The question of how neural networks can produce accurate sequencing is an unsolved problem in neuroscience. Whereas Project 1 deals with the question of how SPW-Rs are initiated, Project 2 deals with how memory sequences are produced. Critical to this project is the need to determine the firing patterns of pyramidal cells and each of the major interneuron types during SPW-Rs. A large number of powerful new optical methods will be used to obtain this data. A second class of experiments will use optogenetic inactivation methods to determine whether the sequencing operation can be produced by events in CA2/CA3 alone, or whether the dentate gyrus (DG) can also influence SPW-R content. We further hope to reveal the basis of important discretization processes that govern SPW-R-related memory sequencing. There have been indications that memory readout during SPW-Rs is discretized by gamma oscillations - in Project 2, we will attempt to verify this and shed light on the basis of this timing operation. Other experiments will provide information relevant to understanding SPW-R termination processes. Collectively, this information, in conjunction with the analysis and simulations proposed in Project 5, is likely to answer fundamental questions about the cellular and network mechanisms governing the recruitment and sequencing of memory content during SPW-R-related replay.

SPW-R的记忆功能主要依赖于记忆项的快速顺序重放 (e.g.沿着探索路径的空间位置）。神经网络如何产生 精确测序是神经科学中未解决的问题。而项目1处理的是 关于SPW-R如何启动的问题，项目2涉及如何产生记忆序列。 这个项目的关键是需要确定锥体细胞的放电模式， SPW-R期间的主要中间神经元类型。大量强大的新光学方法将被 用于获取这些数据。第二类实验将使用光遗传失活方法 确定排序操作是否可以单独由CA 2/CA 3中的事件产生，或者 齿状回（DG）是否也能影响SPW-R含量。我们还希望揭示 重要的离散化过程的基础上，支配SPW-R相关的内存排序。那里 有迹象表明，SPW-R期间的存储器读出是由伽马振荡离散化的- 在项目2中，我们将尝试验证这一点，并在此定时操作的基础上阐明这一点。其他 实验将提供相关的信息，了解SPW-R终止过程。 总的来说，这些信息，结合项目中提出的分析和模拟， 5，可能会回答有关细胞和网络机制的基本问题， SPW-R相关重放期间记忆内容的募集和排序。