Mechanisms of SPW-R sequencing and termination

SPW-Rs的记忆功能在很大程度上依赖于记忆项目的快速顺序回放 (例如沿探索路径的空间位置)。神经网络如何产生 准确测序在神经科学中是一个悬而未决的问题。鉴于项目1处理的是 关于SPW-R是如何启动的问题，项目2讨论了记忆序列是如何产生的。 这个项目的关键是需要确定锥体细胞和每个锥体细胞的放电模式 SPW-R期间的主要中间神经元类型。一大批强大的新光学方法将被 用于获取此数据。第二类实验将使用光基因灭活方法。 以确定测序操作是否可以仅由CA2/CA3中的事件产生，或者 齿状回(DG)是否也影响SPW-R含量。我们进一步希望能够揭示出 控制SPW-R相关内存排序的重要离散化过程的基础。那里 已经有迹象表明，SPW-R期间的存储器读出是由伽马振荡离散的- 在项目2中，我们将尝试验证这一点，并在此计时操作的基础上阐明。其他 实验将提供与理解SPW-R终止过程相关的信息。 总的来说，这些信息与项目中建议的分析和模拟相结合 5，可能回答有关管理蜂窝和网络机制的基本问题 SPW-R相关重播中记忆内容的招募和排序。

The memory function of SPW-Rs depends critically on rapid sequential replay of memory items (e.g. spatial positions along an explored path). The question of how neural networks can produce accurate sequencing is an unsolved problem in neuroscience. Whereas Project 1 deals with the question of how SPW-Rs are initiated, Project 2 deals with how memory sequences are produced. Critical to this project is the need to determine the firing patterns of pyramidal cells and each of the major interneuron types during SPW-Rs. A large number of powerful new optical methods will be used to obtain this data. A second class of experiments will use optogenetic inactivation methods to determine whether the sequencing operation can be produced by events in CA2/CA3 alone, or whether the dentate gyrus (DG) can also influence SPW-R content. We further hope to reveal the basis of important discretization processes that govern SPW-R-related memory sequencing. There have been indications that memory readout during SPW-Rs is discretized by gamma oscillations - in Project 2, we will attempt to verify this and shed light on the basis of this timing operation. Other experiments will provide information relevant to understanding SPW-R termination processes. Collectively, this information, in conjunction with the analysis and simulations proposed in Project 5, is likely to answer fundamental questions about the cellular and network mechanisms governing the recruitment and sequencing of memory content during SPW-R-related replay.