Dynamics of Endomembrane Docking and Fusion

摘要摘要 COPII介导的内质网到高尔基体的膜交通首次使用粗细胞提取液重建了30年 那是以前的事了。20年前，COPII囊泡的形成是用纯蛋白质和脂类来概括的。 然而，COPII囊泡的消耗--它们的去涂层、捆绑、对接和与ACEP的融合-- 对于TOR隔室，人们仍然知之甚少。这在很大程度上是因为原油运输分析 30年前开发的产品并没有被使用化学定义的成分的系统所取代。 在这个项目中，我们介绍了一个这样的系统，并用它来检验关于生化序列的假设 COPII囊泡运输中的亚反应、执行这些亚反应的成分和机制 它们通过这一点发挥作用。

Abstract Summary COPII mediated ER-to-golgi membrane traffic was first reconstituted using crude cell extracts thirty years ago. Twenty years ago, the formation of COPII vesicles was recapitulated using pure proteins and lipids. However, the consumption of COPII vesicles – their uncoating, tethering, docking, and fusion with accep- tor compartments, is still poorly understood. This is in large measure because the crude transport assay developed thirty years ago has not been supplanted by a system employing chemically defined components. In this Project we introduce such a system and use it to test hypotheses about the sequence of biochemical subreactions in COPII vesicle traffic, the components that execute these subreactions, and the mechanisms through which they function.