Dynamics of Endomembrane Docking and Fusion

内膜对接和融合的动力学

• 批准号: 10207177
• 负责人: Alexey Jarrell Merz
• 金额: $ 37.34万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10207177
• 关键词: Address; Antibodies; Area; Biochemical; Biochemical Genetics; Biological Assay; Biophysics; Cell Extracts; Cell physiology; Cells; Chemicals; Coated vesicle; Complement; Complex; Consumption; Data; Disease; Dissociation; Docking; Elements; Endoplasmic Reticulum; Genes; Genetic; Genetic Screening; Genomic approach; Golgi Apparatus; Golgi Targeting; Grant; Guanine Nucleotide Exchange Factors; Guanosine Triphosphate Phosphohydrolases; HIV; Hand; Hormone secretion; Lesion; Lipids; Measures; Mediating; Membrane; Membrane Protein Traffic; Modeling; Molecular Conformation; Nucleotides; Pathway interactions; Phosphatidylinositols; Process; Proteins; Public Health; Publishing; Recycling; Research; SNAP receptor; Saccharomycetales; Structure; System; TRAPP transport protein particle; Testing; Vesicle; Virus Assembly; Virus Diseases; Yeasts; cofactor; experimental study; functional genomics; fundamental research; golgin; human disease; insight; prevent; rab GTP-Binding Proteins; reconstitution; therapeutic development; vesicle transport; virus genetics

Abstract Summary COPII mediated ER-to-golgi membrane traffic was first reconstituted using crude cell extracts thirty years ago. Twenty years ago, the formation of COPII vesicles was recapitulated using pure proteins and lipids. However, the consumption of COPII vesicles – their uncoating, tethering, docking, and fusion with accep- tor compartments, is still poorly understood. This is in large measure because the crude transport assay developed thirty years ago has not been supplanted by a system employing chemically defined components. In this Project we introduce such a system and use it to test hypotheses about the sequence of biochemical subreactions in COPII vesicle traffic, the components that execute these subreactions, and the mechanisms through which they function.

抽象概括 COPII介导的ER到高尔基体膜的运输首次使用粗细胞提取物重建， 前二十年前，COPII囊泡的形成是使用纯蛋白质和脂质重现的。 然而，COPII囊泡的消耗--它们的脱壳、束缚、对接以及与accep的融合-- 对于分隔区，仍然知之甚少。这在很大程度上是因为粗转运分析 30年前开发的系统还没有被使用化学成分的系统所取代。 在这个项目中，我们介绍了这样一个系统，并使用它来测试有关生物化学序列的假设。 COPII囊泡运输中的子反应，执行这些子反应的组件以及机制 通过它来运作。