MOLECULAR BASIS OF PILUS-MEDIATED GONOCOCCAL ADHESION
MOLECULAR BASIS OF PILUS-MEDIATED GONOCOCCAL ADHESION
批准号:
10363679
负责人:
Alexey Jarrell Merz
金额:
$19.44万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-03-03 至 2023-02-28
关键词:
AdherenceAdhesionsAdhesivesAffinityAnimal ModelAntibioticsBacteriaBacterial AdhesinsBacterial MeningitisBindingBiochemicalBiogenesisBioinformaticsC-terminalCellsDataDevelopmentDockingDrug resistanceEpithelialEpithelial CellsEpitopesFiberGeneticGoalsGonorrheaHumanIn VitroInfectionLegionella pneumophilaLengthMediatingModelingMolecularMucous MembraneN-terminalNeisseriaNeisseria gonorrhoeaeNeisseria meningitidisPilumPrevalenceProcessProteinsPseudomonas aeruginosaPublic HealthPublishingReagentRecombinantsSignal TransductionSpecificityStructureTestingTherapeuticTimeTissuesTropismVaccinesVirulenceVirulence FactorsWorkbaseexperimental studygenetic testingimprovedin vivoneutralizing antibodypathogenreceptorresponse
中文摘要
项目总结
英文摘要
PROJECT SUMMARY
Neisseria gonorrhoeae (GC) is a growing public health problem which is likely to worsen due to increasing
antibiotic resitance and the lack of a vaccine. Type IV pili (Tfp) are known to be essential for GC virulence in
humans, and there is strong evidence that Tfp bind to high-affinity receptors on human cells. However, the
identities of both the specific GC protein(s) and host receptor(s) responsible for high-affinity adherence are not
known. Twenty years of pubished experiments strongly support the hypothesis that the low-abundance protein
PilC is the high-affinity adhesin presented by GC Tfp. However, little is known about the overall structure of
PilC, the mechanisms through which it associates with Tfp, and the host receptors that it presumably engages.
In this project we will use in vivo genetic tests and in vitro biochemical analyses to test models of PilC
functional organization, and we will use the resulting information and reagents to begin the process of
identifying high-affinity receptors on human mucosal epithelial cells. The impacts of this project will include
clarifying the mechanisms of PilC association with pili, the mechanisms through which PilC influences pilus
biogenesis, and the mechanisms through which GC and the closely related pathogen N. meningitidis adhere to
human epithelial tissues to initiate infection.
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会议论文
MECHANISMS OF AP-3 FUNCTION IN VESICLE FORMATION AND GOLGI MATURATION
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批准号:10456623
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项目类别:
-
资助金额:$47.0万
-
财政年份:2019
-
负责人:Alexey Jarrell Merz
-
依托单位:
MECHANISMS OF AP-3 FUNCTION IN VESICLE FORMATION AND GOLGI MATURATION
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批准号:10226217
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项目类别:
-
资助金额:$46.65万
-
财政年份:2019
-
负责人:Alexey Jarrell Merz
-
依托单位:
MECHANISMS OF AP-3 FUNCTION IN VESICLE FORMATION AND GOLGI MATURATION
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批准号:9815765
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项目类别:
-
资助金额:$48.95万
-
财政年份:2019
-
负责人:Alexey Jarrell Merz
-
依托单位:
MECHANISMS OF AP-3 FUNCTION IN VESICLE FORMATION AND GOLGI MATURATION
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批准号:10017291
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项目类别:
-
资助金额:$47.0万
-
财政年份:2019
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负责人:Alexey Jarrell Merz
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依托单位:
PROTEIN PHOSPHORYLATION IN YEAST VACUOLE FUSION
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批准号:8171286
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项目类别:
-
资助金额:$0.24万
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财政年份:2010
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负责人:Alexey Jarrell Merz
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依托单位:
PROTEIN PHOSPHORYLATION IN YEAST VACUOLE FUSION
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批准号:7957800
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项目类别:
-
资助金额:$0.33万
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财政年份:2009
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负责人:Alexey Jarrell Merz
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依托单位:
PROTEIN INTERACTIONS WITH VACUOLE TARGETING MACHINERY
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批准号:7957852
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项目类别:
-
资助金额:$0.74万
-
财政年份:2009
-
负责人:Alexey Jarrell Merz
-
依托单位:
FLUORESCENCE MICROSCOPY OF PROTEINS INVOLVED IN GOLGI-TO-VACUOLE VESICLE TRAFFIC
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批准号:7723724
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项目类别:
-
资助金额:$1.95万
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财政年份:2008
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负责人:Alexey Jarrell Merz
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依托单位:
Dynamics of Endomembrane Docking and Fusion
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批准号:10386618
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项目类别:
-
资助金额:$20.93万
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财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of endomembrane docking and fusion
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批准号:7368053
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项目类别:
-
资助金额:$28.12万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:8235481
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项目类别:
-
资助金额:$34.17万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of endomembrane docking and fusion
-
批准号:7192517
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项目类别:
-
资助金额:$28.09万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:9893719
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项目类别:
-
资助金额:$34.0万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:10381726
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项目类别:
-
资助金额:$37.34万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of endomembrane docking and fusion
-
批准号:7078232
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项目类别:
-
资助金额:$28.87万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:8811134
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项目类别:
-
资助金额:$32.87万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:8473879
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项目类别:
-
资助金额:$31.72万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:10207177
-
项目类别:
-
资助金额:$37.34万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:10598023
-
项目类别:
-
资助金额:$37.34万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
Dynamics of Endomembrane Docking and Fusion
-
批准号:8611926
-
项目类别:
-
资助金额:$32.87万
-
财政年份:2006
-
负责人:Alexey Jarrell Merz
-
依托单位:
海外基金