Dynamics of Endomembrane Docking and Fusion

抽象概括 COPII介导的ER到高尔基体膜的运输首次使用粗细胞提取物重建， 前二十年前，COPII囊泡的形成是使用纯蛋白质和脂质重现的。 然而，COPII囊泡的消耗--它们的脱壳、束缚、对接以及与accep的融合-- 对于隔间，人们仍然知之甚少。这在很大程度上是因为粗转运分析 30年前开发的系统还没有被使用化学成分的系统所取代。 在这个项目中，我们介绍了这样一个系统，并使用它来测试有关生物化学序列的假设。 COPII囊泡运输中的子反应，执行这些子反应的组件，以及机制 通过它来运作。

Abstract Summary COPII mediated ER-to-golgi membrane traffic was first reconstituted using crude cell extracts thirty years ago. Twenty years ago, the formation of COPII vesicles was recapitulated using pure proteins and lipids. However, the consumption of COPII vesicles – their uncoating, tethering, docking, and fusion with accep- tor compartments, is still poorly understood. This is in large measure because the crude transport assay developed thirty years ago has not been supplanted by a system employing chemically defined components. In this Project we introduce such a system and use it to test hypotheses about the sequence of biochemical subreactions in COPII vesicle traffic, the components that execute these subreactions, and the mechanisms through which they function.