FLUORESCENCE MICROSCOPY OF PROTEINS INVOLVED IN GOLGI-TO-VACUOLE VESICLE TRAFFIC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们感兴趣的是已知的液泡融合因子在AP-3囊泡运输途径中的作用。虽然我们已经确定了AP-3囊泡涂层复合体和一组融合因子之间的体外相互作用，但这些相互作用是否发生在体内仍不清楚。我们的目标是使用荧光显微镜来确定AP-3与这些融合因子在体内的共定位，以及这可能发生在哪个细胞室。这些信息将有助于深入了解AP-3是否与体内的融合因子相互作用，以及这些相互作用的可能功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the role of known vacuole fusion factors within the AP-3 vesicle trafficking pathway. While we have identified in vitro interactions between the AP-3 vesicle coat complex and a subset of fusion factors, whether these interactions occur in vivo remains unknown. Our goal is to use fluorescence microscopy to determine the colocalization of AP-3 with these fusion factors in vivo and at which cellular compartment this may take place. This information will provide insight into whether AP-3 interacts with the fusion factors in vivo and the possible function of these interactions.