Tissue Processing and Histology

摘要 组织加工与组织学核心 组织处理和组织学核心将继续服务于加州大学圣迭戈分校视觉研究人员的需求 提供专门的组织处理设备和具有眼科经验的全职组织技师 组织学和免疫组织化学。组织处理和组织学核心支持石蜡和 冰冻切片处理和各种染色策略。该核心还为视觉研究人员提供了1) 根据研究目的选择固定的建议；2)研究样本的石蜡和冷冻封堵；3) 石蜡或冷冻组织学切片准备，定制染色，4)协助正确解释 以及用于电子显微镜评估的感兴趣区域的精确显微采样和5)高含量 共聚焦成像用于药物筛选和组织标本成像。

ABSTRACT Tissue Processing and Histology Core The Tissue Processing and Histology Core will continue to serve the needs of UCSD vision researchers by providing dedicated tissue processing equipment and a full-time histotechnician experienced in eye histology and immunohistochemistry. The Tissue Processing and Histology core supports both paraffin and cryosection processing and various staining strategies. The core also provides vision researchers with 1) advice for fixation selection depending on study purpose; 2) paraffin and cryo blocking of study samples; 3) paraffin or cryo histology slide preparation with customized staining, 4) assistance for proper interpretation and precision microsampling of the region of interest for electron microscopy evaluation and 5) high content confocal imaging for drug screening and histologic specimen imaging.