Tissue Processing and Histology

组织加工和组织学

• 批准号: 9769046
• 负责人: LINGYUN CHENG
• 金额: $ 13.39万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9769046
• 关键词: Animals; Center Core Grants; Custom; Disease; Drug Screening; Electron Microscopy; Equipment; Evaluation; Eye; Eye diseases; Functional disorder; Genetic Engineering; Histologic; Histology; Image; Immunohistochemistry; Location; Modeling; Paraffin; Pharmacology; Preparation; Research Personnel; Sampling Studies; Services; Slide; Stains; Techniques; Time; Tissues; Vision; Vision research; base; confocal imaging; experience; histological specimens; interest; microscopic imaging; sample fixation; tissue processing

ABSTRACT Tissue Processing and Histology Core The Tissue Processing and Histology Core will continue to serve the needs of UCSD vision researchers by providing dedicated tissue processing equipment and a full-time histotechnician experienced in eye histology and immunohistochemistry. The Tissue Processing and Histology core supports both paraffin and cryosection processing and various staining strategies. The core also provides vision researchers with 1) advice for fixation selection depending on study purpose; 2) paraffin and cryo blocking of study samples; 3) paraffin or cryo histology slide preparation with customized staining, 4) assistance for proper interpretation and precision microsampling of the region of interest for electron microscopy evaluation and 5) high content confocal imaging for drug screening and histologic specimen imaging.

摘要 组织处理和组织学核心 组织处理和组织学核心将继续服务于UCSD视觉研究人员的需求， 提供专门的组织处理设备和一名在眼科领域经验丰富的全职组织技术人员， 组织学和免疫组织化学。组织处理和组织学核心支持石蜡和 冷冻切片处理和各种染色策略。该核心还为视觉研究人员提供了1） 根据研究目的提供固定选择建议; 2）研究样本的石蜡和冷冻封闭; 3） 定制染色的石蜡或冷冻组织学载玻片制备，4）协助正确判读 用于电子显微镜评价的感兴趣区域的精确微取样和5）高含量 用于药物筛选和组织学样本成像的共焦成像。