Determination of biomarkers of iron status and inflammation in saliva.

唾液中铁状态和炎症生物标志物的测定。

• 批准号: 10303822
• 负责人: Saurabh Mehta
• 金额: $ 23.55万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-09 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10303822
• 关键词: Accounting; Acids; Active Biological Transport; Address; Affect; Age; Anemia; Archives; Back; Base Ratios; Biological; Biological Assay; Biological Markers; Blood; Blood specimen; Body measure procedure; Breast Feeding; Bypass; C-reactive protein; Centers for Disease Control and Prevention (U.S.); Child; Collection; Communities; Development; Diagnostic; Diffusion; Elderly; Equation; Equipment; Ferritin; Food; Future; Goals; HSV glycoprotein C; Health; Heterogeneity; Human Resources; India; Individual; Inflammation; Inflammatory; Infrastructure; Intake; International; Intervention; Iron; Lactation; Link; Malnutrition; Measures; Methods; Morbidity - disease rate; Mothers; National Health and Nutrition Examination Survey; Nutrient; Nutrition Assessment; Nutritional; Nutritional status; Orosomucoid; Outcome; Oxygen; Pennisetum; Performance; Physiological; Population; Production; Program Evaluation; Proteins; Public Health; Randomized; Research; Resistance; Resources; Risk; Saliva; Salivary; Sampling; Serum; Site; Surveillance Program; Surveys; TFRC gene; Testing; Training; Ultrafiltration; Wheat; Woman; Work; base; biomarker selection; blood-based biomarker; cohort; community setting; cooking; cost efficient; diagnostic accuracy; feeding; hepcidin; high risk; immune function; improved; indexing; innovation; iron deficiency; mortality; nutrition; point-of-care diagnostics; population survey; pregnant; reproductive; research and development; response; saliva sample; salivary assay; sample collection

PROJECT SUMMARY Biomarker determination for both nutritional status and inflammation has classically relied on blood-based biomarkers. Blood sampling has associated challenges including being partially invasive, requiring specially trained personnel and equipment for collection and processing, and having resistance to blood collection in some communities. Our long-term goal is to evaluate salivary biomarkers as an option to address issues common with blood sampling. Our overall objectives in this application are to determine salivary and serum biomarkers of iron and inflammation status in a population with expected burden of malnutrition and inflammation. We hypothesize that salivary biomarkers will demonstrate sufficient diagnostic performance for determining the degree of inflammation and iron status relative to serum biomarkers. We will test our hypothesis with the following specific aims: 1) In a resource-limited setting with heterogeneity in iron status, compare salivary versus serum biomarkers accounting for inflammation, 2) Evaluate the utility of saliva for determination of the ratio-based body iron index, and 3) Measure changes in salivary biomarkers and the body iron index in response to an iron intervention. Our approach includes domestic pilot work and leveraging a cohort of mothers and their young children in India participating in a feeding trial including biofortified high iron pearl millet. We also have paired serum-saliva samples from a survey of women of reproductive age participating in a CDC-supported periconceptional surveillance program at the same site in India as a potential back up. In our opinion, this research is innovative because the development of salivary biomarkers of inflammatory and nutritional status, including the ratio-based body iron index, would facilitate easier, cheaper, and more acceptable sampling and assessment of an individual’s or population’s status. These contributions are significant because they could dramatically improve biological sampling and assessment for nutritional and inflammatory assessment, which remain significant public health burdens globally and have challenges with assessment of high-risk individuals. Such developments would open up future avenues of research and development in salivary biomarkers and diagnostics.

项目摘要 营养状态和炎症的生物标志物测定传统上依赖于基于血液的 生物标志物。血液采样具有相关的挑战，包括部分侵入性，需要特殊的 接受过采集和处理的人员和设备，在某些情况下对血液采集有抵抗力， 社区.我们的长期目标是评估唾液生物标志物，作为解决常见问题的一种选择， 血样采集我们在这项应用中的总体目标是确定唾液和血清中铁的生物标志物 和炎症状态。我们假设 唾液生物标志物将显示出足够的诊断性能，用于确定 炎症和相对于血清生物标志物的铁状态。我们将用以下具体数据来检验我们的假设： 目的：1）在资源有限且铁状态异质性的环境中，比较唾液与血清生物标志物 考虑炎症，2）评估唾液用于测定基于比率的身体铁指数的效用， 和3）测量唾液生物标志物和身体铁指数响应于铁干预的变化。 我们的方法包括国内试点工作和利用印度的一群母亲及其幼儿 参与包括生物强化高铁珍珠小米的饲养试验。我们还有成对的血清唾液 来自参加CDC支持的围概念调查的育龄妇女的样本 在印度的同一地点进行监视计划，作为潜在的备份。在我们看来，这项研究是创新的 因为唾液炎症和营养状况的生物标志物的发展，包括基于比率的 身体铁指数，将有助于更容易，更便宜，更可接受的采样和评估， 个人或群体的地位。这些贡献意义重大，因为它们可以显著改善 用于营养和炎症评估的生物采样和评估，这仍然是重要的公共 全球健康负担，并在评估高风险个体方面面临挑战。这些发展将 开辟了唾液生物标志物和诊断的未来研究和开发途径。