Determination of biomarkers of iron status and inflammation in saliva.

项目总结 营养状态和炎症的生物标记物的确定传统上依赖于血液 生物标志物。采血也有相关的挑战，包括部分有创，需要特别 训练有素的采集、加工人员和设备，部分地区对采血有抵抗力 社区。我们的长期目标是评估唾液生物标记物作为解决常见问题的一种选择 采血。我们在这项应用中的总体目标是确定唾液和血清中铁的生物标记物 以及预期有营养不良和炎症负担的人群的炎症状况。我们假设 唾液生物标志物将显示出足够的诊断性能来确定 与血清生物标记物相关的炎症和铁状态。我们将使用以下具体内容来验证我们的假设 目的：1)在资源有限且铁状态不同的环境中，比较唾液和血清生物标记物 考虑炎症，2)评估唾液用于确定基于比率的体铁指数的效用， 以及3)测量唾液生物标记物和身体铁指数在铁干预后的变化。 我们的方法包括国内试点工作和利用印度的一群母亲及其年幼的孩子 参加包括生物强化高铁珍珠谷子在内的饲养试验。我们还有配对的血清-唾液 参加美国疾病控制与预防中心支持的围生期妊娠的育龄妇女调查样本 在印度的同一地点进行监视计划，作为潜在的后备。在我们看来，这项研究具有创新性。 因为唾液生物标志物的发展反映了炎症和营养状况，包括基于比率的 身体铁指数，将有助于更容易、更便宜和更可接受的采样和评估 个人或群体的地位。这些贡献是重要的，因为它们可以显著提高 用于营养和炎症评估的生物采样和评估，这仍然是重要的公众 在全球范围内造成健康负担，并在评估高危个人方面面临挑战。这样的发展将会 开辟唾液生物标记物和诊断学的未来研究和开发途径。

PROJECT SUMMARY Biomarker determination for both nutritional status and inflammation has classically relied on blood-based biomarkers. Blood sampling has associated challenges including being partially invasive, requiring specially trained personnel and equipment for collection and processing, and having resistance to blood collection in some communities. Our long-term goal is to evaluate salivary biomarkers as an option to address issues common with blood sampling. Our overall objectives in this application are to determine salivary and serum biomarkers of iron and inflammation status in a population with expected burden of malnutrition and inflammation. We hypothesize that salivary biomarkers will demonstrate sufficient diagnostic performance for determining the degree of inflammation and iron status relative to serum biomarkers. We will test our hypothesis with the following specific aims: 1) In a resource-limited setting with heterogeneity in iron status, compare salivary versus serum biomarkers accounting for inflammation, 2) Evaluate the utility of saliva for determination of the ratio-based body iron index, and 3) Measure changes in salivary biomarkers and the body iron index in response to an iron intervention. Our approach includes domestic pilot work and leveraging a cohort of mothers and their young children in India participating in a feeding trial including biofortified high iron pearl millet. We also have paired serum-saliva samples from a survey of women of reproductive age participating in a CDC-supported periconceptional surveillance program at the same site in India as a potential back up. In our opinion, this research is innovative because the development of salivary biomarkers of inflammatory and nutritional status, including the ratio-based body iron index, would facilitate easier, cheaper, and more acceptable sampling and assessment of an individual’s or population’s status. These contributions are significant because they could dramatically improve biological sampling and assessment for nutritional and inflammatory assessment, which remain significant public health burdens globally and have challenges with assessment of high-risk individuals. Such developments would open up future avenues of research and development in salivary biomarkers and diagnostics.