Obesity and COVID-19: Role of Adipose Tissue

肥胖和 COVID-19：脂肪组织的作用

• 批准号: 10302846
• 负责人: TRACEY MCLAUGHLIN
• 金额: $ 23.62万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10302846
• 关键词: 2019-nCoV; ACE2; Address; Adipocytes; Adipose tissue; COVID-19; Cardiac; Cells; Central obesity; Down-Regulation; Fatty acid glycerol esters; Fibrosis; Functional disorder; Goals; Heart; Human; Hypertension; Immune response; Impairment; Infection; Inflammation; Inflammatory Response; Injury; Intestines; Investigational Therapies; Kidney; Link; Liver; Metabolic syndrome; Non obese; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Organ; Outcome; Pharmacology; Play; Predisposition; Proteins; Renin-Angiotensin System; Research; Risk; Risk Factors; Role; SARS-CoV-2 exposure; Viral; Viral Load result; Virus Diseases; Visceral; obese person; receptor; response; subcutaneous; treatment effect; vasoconstriction

PROJECT SUMMARY/ABSTRACT Obesity is a major risk factor for severe outcomes in Covid-19, exceeding the risk associated with type 2 diabetes and hypertension by 3-fold. The underlying reason is unknown. While obese individuals may have an impaired immune response or a predilection towards inflammation, hypertension, and cardiac decompensation due to underlying metabolic syndrome, we hypothesize that a more specific mechanism is at play: ACE2, the receptor for the spike protein on SARS-CoV-2 is highly expressed in subcutaneous and visceral adipose tissue, which may allow for viral entry and replication. Particularly in peri-organ fat depots, inflammation, vasoconstriction and fibrosis as a consequence of viral infection and subsequent downregulation of ACE2 may contribute to organ damage including heart, gut, liver, and kidney. As such, the goal of this project is to determine whether SARS-CoV-2 infects human adipocytes from subcutaneous (SAT), visceral (VAT), epicardial (EAT), and paracardial adipose tissue (PAT), whether infection incites inflammation, and whether pharmacologic compounds that target the renin-angiotensin system (RAS)/ACE2 alter infectivity and inflammation. 1. Assess the ability of SARS-CoV-2 to infect adipocytes and/or other resident cells in adipose tissue (SAT, VAT, EAT, PAT) 2. Profile the inflammatory response in adipose tissue cells exposed to SARS-CoV-2 3. Determine the ability of pharmacologic compounds that target RAS/ACE2 to inhibit infectivity and/or inflammation The results of this research will: 1) address a fundamental and critically-important question: does adipose tissue play a role in the link between obesity and COVID-19, via increased infection and/or inflammation; and 2) evaluate the effects of treatments targeting RAS/ACE2 that may mitigate infectivity and/or inflammation in adipose tissue.

项目总结/文摘