Biogenic Gas Nanostructures As Molecular Imaging Reporters For Ultrasound
Biogenic Gas Nanostructures As Molecular Imaging Reporters For Ultrasound
批准号:
10318929
负责人:
Mikhail Shapiro
金额:
$65.38万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-03-01 至 2023-12-31
关键词:
AchievementAcousticsAddressAreaBacteriaBiochemicalBiologicalBiologyBiosensorBlood CirculationCell physiologyCellsChemicalsContrast MediaDetectionDevelopmentDiagnosticDimensionsElementsEngineeringEscherichia coliEventExtravasationFrequenciesGasesGastrointestinal tract structureGene ExpressionGeneticGenetic EngineeringGoalsImageImaging technologyLaboratoriesMagnetic Resonance ImagingMalignant NeoplasmsMammalian CellMedicineMetalloproteasesMethodsMicrobeMicroscopyModalityModelingMolecularMolecular TargetMultimodal ImagingMusNanostructuresNatureOpticsPhysiologic pulsePredispositionPropertyProteinsReporterReporter GenesResearchResolutionRoleSalmonella typhimuriumSensitivity and SpecificitySignal TransductionSurface PropertiesTechniquesTechnologyTherapeutic AgentsTissuesTransplantationUltrasonographyVesicleWorkanalogbasebiological researchbiomedical imagingcellular imagingcellular targetingcommensal bacteriacostdesignenzyme activityextracellularimaging agentimaging modalityin vivoinnovationinsightmicrobiomemolecular diagnosticsmolecular imagingmulticatalytic endopeptidase complexmultidisciplinarymultiplexed imagingnanonanoscalenon-invasive imagingpressureprogramsresearch and developmentresponsesensorsignal processingsuccesssynthetic biologytargeted imagingtemporal measurementtumortumor xenograftultrasounduptake
中文摘要
项目总结/文摘
英文摘要
PROJECT SUMMARY/ABSTRACT
Ultrasound is among the world's most widely used biomedical imaging technologies due to its relative
simplicity, low cost and ability to visualize deep tissues with high spatial and temporal resolution. However,
ultrasound has historically had a small role in molecular and cellular imaging due to the lack of contrast agents
connected to specific aspects of cellular function such as gene expression. To address this limitation, we are
developing the first acoustic biomolecules – proteins that can be imaged with ultrasound. These constructs are
based on gas vesicles – a unique class of gas-filled proteins from buoyant photosynthetic microbes, which we
adapted as imaging agents for ultrasound in 2014. Since this key initial discovery, our laboratory has led the
development of the emerging field of biomolecular ultrasound by engineering the physical, chemical and
biological properties of gas vesicles to enable multiplexed imaging, cellular targeting and selective detection in
vivo. In parallel, we have worked on transplanting the genetic program encoding gas vesicles into heterologous
hosts, recently succeeding in doing so in commensal bacteria relevant to the mammalian microbiome, while in
parallel making initial progress on expressing gas vesicles in mammalian cells. In addition, we discovered that
gas vesicles can produce susceptibility-weighted MRI contrast erasable by ultrasound, providing an additional
readout modality with unique advantages. Here we propose to build on these insights to advance gas vesicles
as targeted nanoscale contrast agents, mammalian reporter genes and functional sensors for ultrasound. This
work will focus on engineering gas vesicle properties for long-term circulation and extravascular targeting
through the bloodstream, achieving robust expression of gas vesicles as reporter genes in mammalian cells,
developing nonlinear ultrasound pulse sequences to maximize the sensitivity of gas vesicle imaging, and
designing the first acoustic sensors of enzyme activity. The fundamental innovation contained in this research
is that gas vesicle are the first biomolecular, genetically engineered and encoded contrast agent of any kind for
ultrasound. As a result, they have the potential to transform this imaging modality analogously to the way
fluorescent proteins transformed optical microscopy.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
International Symposium on Biomolecular Ultrasound and Sonogenetics
-
批准号:10609240
-
项目类别:
-
资助金额:$2.0万
-
财政年份:2022
-
负责人:Mikhail Shapiro
-
依托单位:
The Future of Molecular MR: A Cellular and Molecular MR Imaging Workshop
-
批准号:10540612
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项目类别:
-
资助金额:$1.75万
-
财政年份:2022
-
负责人:Mikhail Shapiro
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依托单位:
Ultrasonic Genetically Encoded Calcium Indicators for Whole-Brain Neuroimaging
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批准号:10166018
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项目类别:
-
资助金额:$214.21万
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财政年份:2021
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负责人:Mikhail Shapiro
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依托单位:
Sonogenetic Remote Control of Cellular Function
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批准号:10261864
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项目类别:
-
资助金额:$117.25万
-
财政年份:2021
-
负责人:Mikhail Shapiro
-
依托单位:
Sonogenetic Remote Control of Cellular Function
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批准号:10488296
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项目类别:
-
资助金额:$117.25万
-
财政年份:2021
-
负责人:Mikhail Shapiro
-
依托单位:
Sonogenetic Remote Control of Cellular Function
-
批准号:10676282
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项目类别:
-
资助金额:$117.25万
-
财政年份:2021
-
负责人:Mikhail Shapiro
-
依托单位:
Acoustically targeted molecular control of cell type specific neural circuits in non-human primates
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批准号:9804641
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项目类别:
-
资助金额:$116.22万
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财政年份:2019
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负责人:Mikhail Shapiro
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依托单位:
Molecular Functional Ultrasound for Non-Invasive Imaging and Image-Guided Recording and Modulation of Neural Activity
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批准号:9605856
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项目类别:
-
资助金额:$10.24万
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财政年份:2016
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负责人:Mikhail Shapiro
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依托单位:
Dissecting human brain circuits in vivo using ultrasonic neuromodulation
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批准号:8828517
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项目类别:
-
资助金额:$47.19万
-
财政年份:2014
-
负责人:Mikhail Shapiro
-
依托单位:
Biogenic Gas Nanostructures As Molecular Imaging Reporters For Ultrasound
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批准号:8766150
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项目类别:
-
资助金额:$33.95万
-
财政年份:2014
-
负责人:Mikhail Shapiro
-
依托单位:
Dissecting human brain circuits in vivo using ultrasonic neuromodulation
-
批准号:8935955
-
项目类别:
-
资助金额:$49.74万
-
财政年份:2014
-
负责人:Mikhail Shapiro
-
依托单位:
Biogenic Gas Nanostructures As Molecular Imaging Reporters For Ultrasound
-
批准号:9115466
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项目类别:
-
资助金额:$32.1万
-
财政年份:2014
-
负责人:Mikhail Shapiro
-
依托单位:
Biogenic Gas Nanostructures As Molecular Imaging Reporters For Ultrasound
-
批准号:8892182
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项目类别:
-
资助金额:$31.81万
-
财政年份:2014
-
负责人:Mikhail Shapiro
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依托单位:
海外基金