Isolation and Assessment of Blood-Circulating Cancer Exosomes with LSS and SERS Lab on a Chip Optical Spectroscopic Instrument

使用芯片光学光谱仪器上的 LSS 和 SERS 实验室分离和评估血液循环癌症外泌体

• 批准号: 10328506
• 负责人: Lev T Perelman
• 金额: $ 63.73万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10328506
• 关键词: Address; Adopted; Algorithms; Apoptotic; Area; Biochemical; Biogenesis; Biological; Biomedical Engineering; Blood; Blood Circulation; Blood specimen; Cancer Biology; Cancer Center; Cancerous; Cell Culture Techniques; Cells; Cellular biology; Clinical; Clinical Data; Clinical Management; Clinical Research; Collaborations; DNA; Data; Development; Diagnosis; Doctor of Philosophy; Endocytic Vesicle; Endoscopy; Epithelial; Gastroenterologist; Gastrointestinal tract structure; Goals; Human; Imaging Device; Indiana; Industrialization; Lab-On-A-Chips; Laboratories; Light; Light-Scattering Spectroscopy; Liquid substance; Malignant Neoplasms; Malignant neoplasm of pancreas; Mathematics; Measurement; Measures; Medicine; Membrane; Messenger RNA; Methodology; Methods; MicroRNAs; Microfabrication; Microfluidic Microchips; Molecular; Monitor; Nature; Neoplasm Circulating Cells; Neoplasm Metastasis; Normal Cell; Oncogenic; Oncology; Optics; Organ; Organelles; Pathologist; Peripheral; Physiological; Plasma; Primary Neoplasm; Property; Proteins; Raman Spectrum Analysis; Refractive Indices; Research; Sampling; Scientist; Screening for cancer; Shapes; Signal Pathway; Site; Source; Spectrum Analysis; Statistical Data Interpretation; Surface; System; Systems Development; Techniques; Testing; Universities; Vesicle; Vision; algorithm development; angiogenesis; base; cancer cell; cancer genetics; cell free DNA; chemical fingerprinting; clinical application; clinical imaging; decision making algorithm; early detection biomarkers; exosome; experience; improved; insight; instrument; instrumentation; interest; laboratory experiment; light scattering; microvesicles; molecular marker; neoplastic cell; novel; pancreatic cancer patients; physical property; polarized light; predictive marker; response; single molecule; tumor; tumor progression; tumorigenesis; vibration

Project Summary Shed into the peripheral circulation from a primary tumor, circulating tumor cells (CTCs) are limited by their extremely sparse concentrations in blood. In contrast, molecular markers secreted by tumor cells into the vasculature are present in much higher concentrations and could provide important insight on cancer cell biology and predictive biomarkers for early detection, progression and metastasis. These markers, including soluble proteins, cell-free DNA (cfDNA), and circulating microvesicles and exosomes have been shown to contain valuable information on the molecular state of often difficult to access tumor sites. Unfortunately, the source of the soluble proteins found in blood cannot be determined, resulting in the high false-positive rates, while significantly more specific cfDNA often reflect the dying or apoptotic cells. Exosomes, small membrane vesicles of endocytic origin, show the greatest potential as an easily accessible biomarker for early cancer detection and monitoring due to their unique properties, including their stability in biological fluids and their potential to be efficiently isolated. Cancer cells release more exosomes than normal cells and exosomes secreted from tumor cells can promote tumor progression, survival, invasion and angiogenesis. In this application we propose to develop an optical spectroscopic technique, which combines light scattering spectroscopy (LSS) and surface enhanced Raman spectroscopy (SERS) for highly rigorous isolation and assessment of blood-circulating cancer exosomes for early cancer detection in a rapid and inexpensive lab-on-a-chip system. As it is shown in this application, not only LSS can separate exosomes from microvesicles, the capability not available at the moment, but it also can differentiate various types of exosomes originated in different organs of the digestive tract, while SERS is capable of differentiating exosomes secreted by cancerous and normal cells of the same organ.

项目总结

项目成果

Two-photon polymerization nanofabrication of ultracompact light scattering spectroscopic probe for detection of pre-cancer in pancreatic cyst

双光子聚合纳米加工超小型光散射光谱探针检测胰腺囊肿癌前病变

• DOI: 10.1016/j.optlaseng.2021.106616
• 发表时间: 2021
• 期刊: Optics and Lasers in Engineering
• 影响因子: 4.6
• 作者: Sheil, Conor J.;Khan, Umar;Zakharov, Yuri N.;Coughlan, Mark F.;Pleskow, Douglas K.;Sawhney, Mandeep S.;Berzin, Tyler M.;Cohen, Jonah M.;Glyavina, Maria;Zhang, Lei
• 通讯作者: Zhang, Lei

Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor

利用基于光散射光谱的生物传感器直接从全血中快速检测和鉴定细菌

• DOI: 10.1016/j.snb.2021.130489
• 发表时间: 2021
• 期刊: Sensors and Actuators B: Chemical
• 作者: Qiu, Le;Zhang, Lei;Horowitz, Gary L.;Turzhitsky, Vladimir;Coughlan, Mark F.;Glyavina, Maria;Khan, Umar;Zakharov, Yuri N.;Vitkin, Edward;Itzkan, Irving
• 通讯作者: Itzkan, Irving