Functional Microbiomics Core-Bodduluri

功能微生物组学核心-Bodduluri

• 批准号: 10349568
• 负责人: HARIBABU BODDULURI
• 金额: $ 54.71万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10349568
• 关键词: Address; Adopted; Anaerobic Bacteria; Area; Attention; Award; Bioinformatics; Biological; Biomedical Research; Centers of Research Excellence; Clinical; Communities; Continuing Education; Core Facility; Data Analyses; Development; Dietary Intervention; Disease; Equipment; Extramural Activities; Fee-for-Service Plans; Fostering; Funding; Generations; Genomic DNA; Genomics; Germ-Free; Gnotobiotic; Goals; Health; Housing; Human Resources; Incubators; Individual; Infection; Inflammation; Inflammation Mediators; Institution; Kentucky; Laboratories; Mentors; Metagenomics; Methodology; Mission; Mus; Outcome; Pathogenesis; Pathogenicity; Phase; Pilot Projects; Process; Protocols documentation; Research; Research Personnel; Research Support; Resources; Ribosomal RNA; Sampling; Services; Sterility; Techniques; Technology; Tissues; Training; Training and Education; Translational Research; United States National Institutes of Health; Universities; Work; animal facility; base; biosafety level 3 facility; experimental study; fecal transplantation; histopathological examination; inflammatory marker; innovation; interest; investigator training; meetings; member; metabolomics; microbial; microbiome; microbiome research; microbiota; mouse model; pathogen; programs; protocol development; rRNA Genes; social health determinants; social relationships; success; tool

Biomedical research areas including the study of metagenomics, inflammation and pathogenesis are resource and technology intense. All COBRE-supported investigators require state of-the-art resources to successfully compete with investigators from other resource-rich institutions for NIH support. The primary goal of the Functional Microbiomics Core (FMC) is to establish an integrated service center that functions as a critical resource for all Microbiome, Inflammation and Pathogenicity COBRE investigators. Since all the COBRE projects utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, the development of a facility to house and maintain germ-free and gnotobiotic mice is crucial to the FMC. An anaerobic culture facility will support research on gnotobiotic mice that requires specific colonization with fecal transplants or defined anaerobic bacteria. Further, in Aim 2, we propose to establish an integrated metagenomics center for microbiota. This facility will prepare genomic DNA following unified protocols and generate 16S rRNA gene amplicon sequences, and provide bioinformatics support for all microbiota analysis. The FMC will also support all COBRE projects with analysis of their biological samples for inflammatory markers as well as histopathological examination of tissue sections. Education and training of junior investigators and the university wide research community in microbiota research is an important goal of the FMC. Towards this goal, the FMC will organize seminars as well as informal meetings that will serve as a forum for highlighting the scientific and technical advances in the field. Finally, the FMC plans to become an independent service center at UofL to support the needs of all researchers in this area of research

生物医学研究领域，包括元基因组学、炎症和发病机制的研究是资源 和技术密集型。所有Cobre支持的调查人员都需要最先进的资源才能成功 与其他资源丰富的机构的调查人员竞争NIH的支持。的主要目标是 功能微生物核心(FMC)是建立一个发挥关键作用的综合服务中心 所有微生物组、炎症和致病性Cobre研究人员的资源。因为所有的科布雷项目 利用小鼠模型探索炎症和发病机制之间的相互关系，以及 微生物区系对这一进程的贡献，开发一种设施来容纳和保持无菌和 灵知生菌小鼠对FMC至关重要。一个厌氧培养设施将支持对灵知生菌小鼠的研究 需要通过粪便移植或确定的厌氧菌进行特定的定植。此外，在目标2中，我们建议 建立微生物区系综合元基因组学中心。该设施将在下列情况下制备基因组DNA 统一协议，生成16S rRNA基因扩增子序列，并为所有 微生物区系分析。FMC还将支持所有Cobre项目，对其生物样本进行分析 炎性标志物以及组织切片的组织病理学检查。青少年的教育和培训 研究人员和大学范围内的研究社区在微生物区系研究是FMC的一个重要目标。 为实现这一目标，FMC将组织研讨会和非正式会议，作为论坛 突出该领域的科学和技术进步。最后，FMC计划成为一个独立的 UofL服务中心，支持所有研究人员在这一领域的需求