Functional Microbiomics Core-Bodduluri

功能微生物组学核心-Bodduluri

• 批准号: 10349568
• 负责人: HARIBABU BODDULURI
• 金额: $ 54.71万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10349568
• 关键词: Address; Adopted; Anaerobic Bacteria; Area; Attention; Award; Bioinformatics; Biological; Biomedical Research; Centers of Research Excellence; Clinical; Communities; Continuing Education; Core Facility; Data Analyses; Development; Dietary Intervention; Disease; Equipment; Extramural Activities; Fee-for-Service Plans; Fostering; Funding; Generations; Genomic DNA; Genomics; Germ-Free; Gnotobiotic; Goals; Health; Housing; Human Resources; Incubators; Individual; Infection; Inflammation; Inflammation Mediators; Institution; Kentucky; Laboratories; Mentors; Metagenomics; Methodology; Mission; Mus; Outcome; Pathogenesis; Pathogenicity; Phase; Pilot Projects; Process; Protocols documentation; Research; Research Personnel; Research Support; Resources; Ribosomal RNA; Sampling; Services; Sterility; Techniques; Technology; Tissues; Training; Training and Education; Translational Research; United States National Institutes of Health; Universities; Work; animal facility; base; biosafety level 3 facility; experimental study; fecal transplantation; histopathological examination; inflammatory marker; innovation; interest; investigator training; meetings; member; metabolomics; microbial; microbiome; microbiome research; microbiota; mouse model; pathogen; programs; protocol development; rRNA Genes; social health determinants; social relationships; success; tool

Biomedical research areas including the study of metagenomics, inflammation and pathogenesis are resource and technology intense. All COBRE-supported investigators require state of-the-art resources to successfully compete with investigators from other resource-rich institutions for NIH support. The primary goal of the Functional Microbiomics Core (FMC) is to establish an integrated service center that functions as a critical resource for all Microbiome, Inflammation and Pathogenicity COBRE investigators. Since all the COBRE projects utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, the development of a facility to house and maintain germ-free and gnotobiotic mice is crucial to the FMC. An anaerobic culture facility will support research on gnotobiotic mice that requires specific colonization with fecal transplants or defined anaerobic bacteria. Further, in Aim 2, we propose to establish an integrated metagenomics center for microbiota. This facility will prepare genomic DNA following unified protocols and generate 16S rRNA gene amplicon sequences, and provide bioinformatics support for all microbiota analysis. The FMC will also support all COBRE projects with analysis of their biological samples for inflammatory markers as well as histopathological examination of tissue sections. Education and training of junior investigators and the university wide research community in microbiota research is an important goal of the FMC. Towards this goal, the FMC will organize seminars as well as informal meetings that will serve as a forum for highlighting the scientific and technical advances in the field. Finally, the FMC plans to become an independent service center at UofL to support the needs of all researchers in this area of research

生物医学研究领域，包括宏基因组学，炎症和发病机制的研究是资源 and technology技术intense密集.所有COBRE-supported调查人员都需要最先进的资源， 与其他资源丰富的机构的研究人员竞争NIH的支持。的首要目标 功能微生物学核心（FMC）是建立一个综合服务中心，作为一个关键的功能， 所有微生物组、炎症和致病性COBRE研究人员的资源。因为所有的眼镜蛇计划 利用小鼠模型探索炎症和发病机制之间的相互关系， 微生物群对这一过程的贡献，一个设施的发展，以容纳和保持无菌， gnotobiotic小鼠对FMC至关重要。厌氧培养设施将支持对无菌小鼠的研究， 需要粪便移植物或确定的厌氧菌的特异性定植。此外，在目标2中，我们建议 建立一个微生物综合宏基因组学中心。该设施将制备基因组DNA， 统一的协议和生成16 S rRNA基因扩增子序列，并提供生物信息学支持，所有 微生物分析FMC还将支持所有COBRE项目，分析其生物样本， 炎性标志物以及组织切片的组织病理学检查。初级教育和培训 研究人员和大学范围内的微生物群研究是FMC的一个重要目标。 为实现这一目标，妇女联合会将组织研讨会和非正式会议，作为论坛， 突出该领域的科学和技术进步。最后，FMC计划成为一个独立的 UofL的服务中心，以支持该研究领域所有研究人员的需求