Functional Microbiomics Core

功能微生物组学核心

• 批准号: 10492098
• 负责人: HARIBABU BODDULURI
• 金额: $ 75.81万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10492098
• 关键词: 3xTg-AD mouse; Administrative Supplement; Adopted; Aging; Alzheimer' s Disease; Area; Award; Behavior; Bioinformatics; Biological; Blood - brain barrier anatomy; Breeding; Centers of Research Excellence; Core Facility; Data Analyses; Dedications; Derivation procedure; Development; Edible Plants; Equipment; Extramural Activities; Fee-for-Service Plans; Fostering; Funding; Future; Genomics; Germ-Free; Gnotobiotic; Goals; Histopathology; Housing; Image; Individual; Inflammation; Inflammation Mediators; Infrastructure; Institution; Joints; Knockout Mice; Laboratories; Mentors; Methodology; Mission; Modeling; Mus; National Institute of General Medical Sciences; Pathogenesis; Pathogenicity; Phase; Play; Process; Protocols documentation; Research; Research Personnel; Research Project Grants; Research Support; Resources; Ribosomal DNA; Role; Sampling; Services; System; Techniques; Testing; Tissues; Training; Training and Education; Transgenic Organisms; Translational Research; Universities; Whole Organism; Work; animal facility; cancer immunotherapy; clinical translation; exosome; experimental study; imaging facilities; in vivo imaging system; innovation; intravital microscopy; metabolomics; microbiome research; microbiota; microscopic imaging; morris water maze; mouse model; multi-photon; nanopore; novel; object recognition; programs; protocol development; success; synthetic polymer Bioplex; tool; tumor immunology

Project Summary/Abstract In this Phase 2 of the Functional Microbiomics, Inflammation and Pathogenicity COBRE, the primary goal of the Functional Microbiomics Core (FMC) is unchanged. We will sustain and enhance the well-established integrated service center that functions as a critical resource for the Research Project Leaders (RPLs) as a priority, and secondarily for all investigators of the Center. Since all new COBRE projects also utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, our well-established germ-free and gnotobiotic mice facility (GMF) will continue to play a central role in services provided by the FMC. We will continue to provide anaerobic culturing of mixed and/or mono bacterial strains to facilitate the specific colonization of the germ-free mice. 16S ribosomal DNA will be profiled with the in-house developed Oxford nanopore sequencing as well as PacBio-long read sequencing tools, and bioinformatic support provided. Our most extensively used resource; multiplex-based analysis of inflammatory mediators in biological samples, will continue to function using the Bio-Plex 200 (Bio-Rad) multiplex system. In addition, several recent advances allow us to augment the services provided by the FMC. First, the recently developed, highly successful rederivation service of transgenic and KO mouse models as germ-free, will greatly expand the scope and utility of the GMF. Related to this service is the development of a new area of research on the role of microbiota in Alzheimer’s Disease (AD) supported by an administrative supplement to the FMC from NIA/NIGMS. The GMF has already rederived several AD mouse models. For this project, the FMC also developed a suite of mouse behavior analysis models including the Morris Water Maze and Novel Object Recognition tests. These services will support all future ageing related research at the center including a new project on the role of edible-plant derived exosomes in restoring the integrity of the Blood Brain Barrier. We also now include imaging support, in particular Multiphoton Intravital Microscopy for imaging of live tissue and an In Vivo Imaging System (IVIS) for live mouse imaging. Besides functioning as a service center, another major goal of the FMC is to promote the education and training of researchers, primarily the RPLs. The FMC will also interact with other Core facilities at the University of Louisville, such as the Genomics, Metabolomics and Bioinformatics core facilities established with the support of other highly successful COBRE/INBRE programs. In particular, the newly funded Cancer Immunology and Immunotherapy COBRE-operated Functional Immunomics Core (FIC) facility also directed by Dr. Bodduluri has several joint initiatives with the FMC including the establishment of a histopathology service center. The FMC will work with these and other UofL resources in order to avoid duplication of equipment, and to train individual investigators in specific protocols, foster innovative protocol development and integrate analysis of data.

项目概要/摘要 在功能微生物组学、炎症和致病性 COBRE 的第二阶段中， 功能微生物组学核心 (FMC) 保持不变。我们将维持并加强完善的综合 服务中心作为研究项目负责人（RPL）的关键资源作为优先事项，以及 其次是中心的所有调查员。由于所有新的 COBRE 项目也利用小鼠模型来探索 炎症与发病机制之间的相互关系以及微生物群对此的贡献 过程中，我们完善的无菌和无菌小鼠设施 (GMF) 将继续在 FMC 提供的服务。我们将继续提供混合和/或单一细菌的厌氧培养 菌株以促进无菌小鼠的特异性定植。 16S 核糖体 DNA 将用 内部开发的Oxford纳米孔测序以及PacBio-long read测序工具，以及 提供生物信息支持。我们使用最广泛的资源；基于多重的炎症分析 生物样品中的介质将继续使用 Bio-Plex 200 (Bio-Rad) 多重系统发挥作用。在 此外，最近的一些进展使我们能够增强 FMC 提供的服务。首先，最近 开发出高度成功的无菌转基因和 KO 小鼠模型再衍生服务，将极大地促进 扩大转基因食品的范围和效用。与这项服务相关的是新研究领域的发展 关于微生物群在阿尔茨海默病 (AD) 中的作用，并得到 FMC 行政补充的支持 来自 NIA/NIGMS。 GMF已经重新衍生了几种AD小鼠模型。对于这个项目，FMC 还 开发了一套小鼠行为分析模型，包括 Morris Water Maze 和 Novel Object 识别测试。这些服务将支持该中心所有未来与老龄化相关的研究，包括一项新的研究 研究可食用植物来源的外泌体在恢复血脑屏障完整性中的作用的项目。我们也 现在包括成像支持，特别是用于活体组织成像的多光子活体显微镜和 In 用于活体小鼠成像的 Vivo 成像系统 (IVIS)。除了作为服务中心之外，另一个主要目标 FMC 的职责是促进研究人员（主要是 RPL）的教育和培训。 FMC还将互动 与路易斯维尔大学的其他核心设施合作，例如基因组学、代谢组学和生物信息学 核心设施是在其他非常成功的 COBRE/INBRE 计划的支持下建立的。特别是， 新资助的癌症免疫学和免疫治疗 COBRE 操作的功能免疫组学核心 (FIC) 同样由 ​​Bodduluri 博士领导的机构与 FMC 开展了多项联合举措，包括建立一个 组织病理学服务中心。 FMC 将与这些和其他伦敦大学资源合作，以避免 设备重复，并对个别研究人员进行具体方案培训，促进创新方案 数据的开发和整合分析。