Functional Microbiomics Core

项目总结/摘要 在功能微生物、炎症和致病性COBRE的第2阶段， 功能微生物学核心（FMC）不变。我们会维持和加强已确立的综合 作为研究项目负责人（RPL）的关键资源的服务中心，以及 其次是中心的所有研究人员。由于所有新的COBRE项目也利用小鼠模型来探索 炎症和发病机制之间的相互关系，以及微生物群对此的贡献 在这一过程中，我们完善的无菌和gnotobiotic小鼠设施（GMF）将继续发挥核心作用， FMC提供的服务。我们将继续提供混合和/或单一细菌的厌氧培养 菌株以促进无菌小鼠的特异性定殖。16 S核糖体DNA将用 内部开发的牛津纳米孔测序以及PacBio-long read测序工具， 提供生物信息支持。我们最广泛使用的资源;基于多路复用的炎症分析 生物样品中的介体，将继续使用Bio-Rad 200（Bio-Rad）多重系统发挥作用。在 此外，最近的几项进展使我们能够增强FMC提供的服务。首先，最近 开发的，非常成功的转基因和KO小鼠模型的无菌再衍生服务，将大大 扩大基因改造食品的范围和效用。与这项服务有关的是一个新的研究领域的发展 关于微生物群在阿尔茨海默病（AD）中的作用，由FMC的行政补充支持 关于NIA/NIGMS GMF已经重新衍生了几种AD小鼠模型。在这个项目中，FMC还 开发了一套小鼠行为分析模型，包括Morris水迷宫和Novel Object 识别测试。这些服务将支持该中心未来所有与老龄化相关的研究，包括一个新的 关于可食用植物来源的外来体在恢复血脑屏障完整性方面的作用的项目。我们也 现在包括成像支持，特别是用于活组织成像的多光子活体显微镜和In 活体成像系统（IVIS）用于活体小鼠成像。除了作为服务中心，另一个主要目标 FMC的主要任务是促进研究人员的教育和培训，主要是研究人员。FMC还将与 与路易斯维尔大学的其他核心设施，如基因组学，代谢组学和生物信息学 在其他非常成功的COBRE/INBRE计划的支持下建立的核心设施。特别是 新资助的癌症免疫学和免疫治疗COBRE-operated功能免疫组学核心（FIC） Bodduluri博士领导的设施与FMC有几项联合倡议，包括建立一个 组织病理学服务中心FMC将与这些和其他UofL资源合作，以避免 设备重复，并对调查员进行具体规程培训，促进创新规程 数据的开发和综合分析。

Project Summary/Abstract In this Phase 2 of the Functional Microbiomics, Inflammation and Pathogenicity COBRE, the primary goal of the Functional Microbiomics Core (FMC) is unchanged. We will sustain and enhance the well-established integrated service center that functions as a critical resource for the Research Project Leaders (RPLs) as a priority, and secondarily for all investigators of the Center. Since all new COBRE projects also utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, our well-established germ-free and gnotobiotic mice facility (GMF) will continue to play a central role in services provided by the FMC. We will continue to provide anaerobic culturing of mixed and/or mono bacterial strains to facilitate the specific colonization of the germ-free mice. 16S ribosomal DNA will be profiled with the in-house developed Oxford nanopore sequencing as well as PacBio-long read sequencing tools, and bioinformatic support provided. Our most extensively used resource; multiplex-based analysis of inflammatory mediators in biological samples, will continue to function using the Bio-Plex 200 (Bio-Rad) multiplex system. In addition, several recent advances allow us to augment the services provided by the FMC. First, the recently developed, highly successful rederivation service of transgenic and KO mouse models as germ-free, will greatly expand the scope and utility of the GMF. Related to this service is the development of a new area of research on the role of microbiota in Alzheimer’s Disease (AD) supported by an administrative supplement to the FMC from NIA/NIGMS. The GMF has already rederived several AD mouse models. For this project, the FMC also developed a suite of mouse behavior analysis models including the Morris Water Maze and Novel Object Recognition tests. These services will support all future ageing related research at the center including a new project on the role of edible-plant derived exosomes in restoring the integrity of the Blood Brain Barrier. We also now include imaging support, in particular Multiphoton Intravital Microscopy for imaging of live tissue and an In Vivo Imaging System (IVIS) for live mouse imaging. Besides functioning as a service center, another major goal of the FMC is to promote the education and training of researchers, primarily the RPLs. The FMC will also interact with other Core facilities at the University of Louisville, such as the Genomics, Metabolomics and Bioinformatics core facilities established with the support of other highly successful COBRE/INBRE programs. In particular, the newly funded Cancer Immunology and Immunotherapy COBRE-operated Functional Immunomics Core (FIC) facility also directed by Dr. Bodduluri has several joint initiatives with the FMC including the establishment of a histopathology service center. The FMC will work with these and other UofL resources in order to avoid duplication of equipment, and to train individual investigators in specific protocols, foster innovative protocol development and integrate analysis of data.