Maintenance of human-associated and environmental Burkholderia in the soil.

土壤中与人类相关和环境的伯克霍尔德氏菌的维护。

• 批准号: 10374595
• 负责人: Nhu Huynh NGUYEN
• 金额: $ 12.39万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10374595
• 关键词: Maintenance; human; associated; environmental; Burkholderia

The human microbiome is a dynamic ecosystem with high influxes of organisms from the surrounding environment, especially the soil. Upon leaving the human body, these organisms enter the soil and persist through some unknown mechanism, and eventually return to the human body, sometimes as pathogenic organisms. This project uses Burkholderia, a genus with members that are pathogens of animals, plants, and perhaps fungi, to serve as a model system, with the specific goal of defining the basic interactions of symbiotic establishment between Burkholderia isolates and their fungal hosts. This project will provide a mechanistic understanding of interactions between these organisms and discover new insights into how human-associated bacteria persist in the soil: Aim 1 will make direct associations among soil fungi and Burkholderia species and compare the genomic patterns that connect animal, plant, and fungal associated species. The results will allow us to understand and monitor new genetic potentials from environmental strains will allow us to make better predictions of the negative impacts that pathogenic microbes have when they return to interact with humans. Aim 2 will identify the molecular mechanism of interactions mediated by fungal exometabolic products. The results will allow make direct connections between fungi as potential alternative hosts that maintain human-associated microbes in the soil. And Aim 3 will test competitive interactions between Burkholderia and other members of the fungal microbiome. By applying ecological theory to fungal-bacterial interactions, we will be able to make inferences into how certain microbes persist in the soil that may translate to other environment such as the human microbiome. Together the results of this project will elucidate poorly understood mechanisms of fungal-bacterial interactions, and assist with the potential development of novel techniques and theoretical concepts that may aid in predicting, preventing, and curing Burkholderia infections.

人类微生物组是一个动态的生态系统，来自周围环境的生物体大量涌入 环境，尤其是土壤。这些生物体离开人体后，会进入土壤并持续存在 通过某种未知的机制，最终返回人体，有时作为致病性 有机体。该项目使用伯克霍尔德杆菌，该属的成员是动物、植物、 也许还有真菌，作为模型系统，其具体目标是定义基本相互作用 伯克霍尔德杆菌分离株与其真菌宿主之间的共生建立。该项目将提供一个 对这些生物体之间相互作用的机械理解，并发现关于如何相互作用的新见解 与人类相关的细菌持续存在于土壤中：目标 1 将在土壤真菌和 伯克霍尔德杆菌物种并比较连接动物、植物和真菌相关的基因组模式 物种。结果将使我们能够了解和监测环境中的新遗传潜力 菌株将使我们能够更好地预测病原微生物在发生时所产生的负面影响 他们回来与人类互动。目标 2 将确定由以下因素介导的相互作用的分子机制： 真菌外代谢产物。结果将允许真菌之间建立潜在的直接联系 在土壤中维持与人类相关的微生物的替代宿主。而《目标3》将考验竞技性 伯克霍尔德杆菌与真菌微生物组其他成员之间的相互作用。通过应用生态 通过真菌-细菌相互作用的理论，我们将能够推断某些微生物如何持续存在 土壤中的微生物可能会转化为其他环境，例如人类微生物组。综合起来的结果是 该项目将阐明人们知之甚少的真菌-细菌相互作用机制，并协助 可能有助于预测、预防、 并治愈伯克霍尔德杆菌感染。