Maintenance of human-associated and environmental Burkholderia in the soil.

人类微生物群是一个动态的生态系统，周围的生物大量涌入。 环境，尤指土壤。离开人体后，这些微生物进入土壤并持续存在 通过一些未知的机制，并最终回到人体，有时作为致病因素 有机体。这个项目使用伯克霍尔德氏菌，这是一个属，其成员是动物，植物， 也许还有真菌，作为一个模型系统，其具体目标是定义 伯克霍尔德氏菌与其真菌寄主共生关系的建立。该项目将提供一个 从机制上理解这些有机体之间的相互作用，并发现新的见解 人类相关细菌持续存在于土壤中：目标1将使土壤真菌和 伯克霍尔德氏菌物种，并比较连接动物、植物和相关真菌的基因组模式 物种。这一结果将使我们能够了解和监测环境中新的遗传潜力 菌株将使我们能够更好地预测病原微生物在以下情况下产生的负面影响 它们会回来与人类互动。目标2将确定相互作用的分子机制，通过 真菌外代谢产物。这一结果将使真菌之间的直接联系成为可能 在土壤中保持与人类相关的微生物的替代宿主。而Aim 3将测试竞争性 伯克霍尔德氏菌与真菌微生物群其他成员之间的相互作用。通过应用生态 真菌-细菌相互作用的理论，我们将能够推断某些微生物如何持续存在 在土壤中，这可能会转化为其他环境，如人类微生物群。将以下结果汇总在一起 这个项目将阐明鲜为人知的真菌-细菌相互作用的机制，并帮助 潜在的新技术和理论概念的发展，可能有助于预测、预防、 治疗伯克霍尔德氏菌感染。

The human microbiome is a dynamic ecosystem with high influxes of organisms from the surrounding environment, especially the soil. Upon leaving the human body, these organisms enter the soil and persist through some unknown mechanism, and eventually return to the human body, sometimes as pathogenic organisms. This project uses Burkholderia, a genus with members that are pathogens of animals, plants, and perhaps fungi, to serve as a model system, with the specific goal of defining the basic interactions of symbiotic establishment between Burkholderia isolates and their fungal hosts. This project will provide a mechanistic understanding of interactions between these organisms and discover new insights into how human-associated bacteria persist in the soil: Aim 1 will make direct associations among soil fungi and Burkholderia species and compare the genomic patterns that connect animal, plant, and fungal associated species. The results will allow us to understand and monitor new genetic potentials from environmental strains will allow us to make better predictions of the negative impacts that pathogenic microbes have when they return to interact with humans. Aim 2 will identify the molecular mechanism of interactions mediated by fungal exometabolic products. The results will allow make direct connections between fungi as potential alternative hosts that maintain human-associated microbes in the soil. And Aim 3 will test competitive interactions between Burkholderia and other members of the fungal microbiome. By applying ecological theory to fungal-bacterial interactions, we will be able to make inferences into how certain microbes persist in the soil that may translate to other environment such as the human microbiome. Together the results of this project will elucidate poorly understood mechanisms of fungal-bacterial interactions, and assist with the potential development of novel techniques and theoretical concepts that may aid in predicting, preventing, and curing Burkholderia infections.