Maintenance of human-associated and environmental Burkholderia in the soil.

土壤中与人类相关和环境的伯克霍尔德氏菌的维护。

• 批准号: 10375333
• 负责人: Nhu Huynh NGUYEN
• 金额: $ 22.99万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10375333
• 关键词: Animals; Bacteria; Biological Models; Burkholderia; Burkholderia Infections; Development; Ecosystem; Environment; Genetic; Genomics; Goals; Health; Human; Human Microbiome; Human body; Maintenance; Mediating; Microbe; Molecular; Monitor; Organism; Pathogenicity; Pattern; Plants; Soil; Techniques; Testing; Translating; fungus; insight; member; microbiome; mycobiome; novel; pathogen; pathogenic microbe; prevent; theories

The human microbiome is a dynamic ecosystem with high influxes of organisms from the surrounding environment, especially the soil. Upon leaving the human body, these organisms enter the soil and persist through some unknown mechanism, and eventually return to the human body, sometimes as pathogenic organisms. This project uses Burkholderia, a genus with members that are pathogens of animals, plants, and perhaps fungi, to serve as a model system, with the specific goal of defining the basic interactions of symbiotic establishment between Burkholderia isolates and their fungal hosts. This project will provide a mechanistic understanding of interactions between these organisms and discover new insights into how human-associated bacteria persist in the soil: Aim 1 will make direct associations among soil fungi and Burkholderia species and compare the genomic patterns that connect animal, plant, and fungal associated species. The results will allow us to understand and monitor new genetic potentials from environmental strains will allow us to make better predictions of the negative impacts that pathogenic microbes have when they return to interact with humans. Aim 2 will identify the molecular mechanism of interactions mediated by fungal exometabolic products. The results will allow make direct connections between fungi as potential alternative hosts that maintain human-associated microbes in the soil. And Aim 3 will test competitive interactions between Burkholderia and other members of the fungal microbiome. By applying ecological theory to fungal-bacterial interactions, we will be able to make inferences into how certain microbes persist in the soil that may translate to other environment such as the human microbiome. Together the results of this project will elucidate poorly understood mechanisms of fungal-bacterial interactions, and assist with the potential development of novel techniques and theoretical concepts that may aid in predicting, preventing, and curing Burkholderia infections.

人体微生物组是一个动态的生态系统，来自周围的生物体大量涌入 环境，尤其是土壤。离开人体后，这些生物进入土壤并持续存在 通过某种未知的机制，最终返回人体，有时作为致病性 有机体该项目使用伯克霍尔德氏菌属，该属的成员是动物，植物， 也许还有真菌，作为一个模型系统，其具体目标是定义 伯克霍尔德氏菌分离株与其真菌宿主之间的共生建立。该项目将提供一个 这些生物体之间的相互作用的机械理解，并发现新的见解如何 人类相关的细菌持续存在于土壤中：目标1将使土壤真菌和 伯克霍尔德氏菌物种和比较基因组模式，连接动物，植物和真菌相关 物种这些结果将使我们能够了解和监测环境中新的遗传潜力。 菌株将使我们能够更好地预测病原微生物的负面影响， 它们会回来与人类互动目的2将确定介导的相互作用的分子机制， 真菌外代谢产物。这些结果将使真菌之间的直接联系成为可能 在土壤中维持人类相关微生物的替代宿主。Aim 3将测试竞争力 伯克霍尔德氏菌与真菌微生物组的其他成员之间的相互作用。通过应用生态 将理论应用于真菌-细菌相互作用，我们将能够推断某些微生物是如何持续存在的 在土壤中，可能会转化为其他环境，如人类微生物组。综合起来， 该项目将阐明真菌-细菌相互作用的知之甚少的机制，并协助 可能有助于预测，预防， 治疗伯克霍尔德氏菌感染